Diet therapy for the treatment of Alzheimer’s disease in view of traditional Persian medicine: A review
Milad
Iranshahi
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Behjat
Javadi
Department of Traditional Pharmacy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2019
eng
Alzheimer’s disease (AD) is a neurodegenerative disease and the leading cause of dementia worldwide. Epidemiological studies support the important role of diet in prevention and improvement of AD. In Traditional Persian Medicine (TPM), there is multiple dietary guidelines to prevent and alleviate dementia and memory impairment. Pharmacological studies have been shown that most of the TPM-recommended dietary items can improve memory and cognitive decline and possess anti-amyloidogenic, etc. activities. Among them, garlic (Allium sativum) and its compounds, S-allyl-cysteine and diallyl-disulfide, coconut (Cocos nucifera) oil, saffron (Crocus sativus) and crocin and crocetin, honey, fish, lemon balm (Melissa officinalis) and its major compounds rosmarinic acid, raisin and resveratrol, rose flowers (Rosa damascna) and geraniol, ginger (Zingiber officinale) and its 6‐gingerol and 6-shogaol, cumin (Cuminum cyminum) and its main component cuminaldehyde have been found to possess stronger anti-AD activities. Most of these items exhibited antioxidant and AChE inhibitory activities and decreased lipid peroxidation. They also possessed anti-amyloidogenic effects, reduced cerebral plaques and Aβ-species, suppressed cerebral inflammation and alterations in tau protein and inhibited Aβ-induced apoptosis through various mechanisms. Noticeably, there are similarities between TPM anti-AD diet and the typical Mediterranean diet whose beneficial effects on AD have been widely demonstrated. Given the importance of traditional medicine systems in discovering new medicines and nutraceuticals for curing ailments, considering TPM anti-AD dietary recommendations in future research would be helpful.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1102
1117
https://ijbms.mums.ac.ir/article_13789_601e266ac57573499fd34835965ebd02.pdf
dx.doi.org/10.22038/ijbms.2019.36505.8694
Antibacterial activity of lactobacilli probiotics on clinical strains of Helicobacter pylori
Parastoo
Rezaee
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
author
Rouha
kasra kermanshahi
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
author
Tahereh
Falsafi
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
author
text
article
2019
eng
Objective(s): Treatment of Helicobacter pylori infection by common drugs may be associated with several problems such as antimicrobial resistance to commonly used antibiotics and side effects of employed drugs. Therefore, exploration of non-chemical compounds which are safer than chemical ones is becoming important as an alternative therapy. The purpose of this study was to evaluate the effects of lactic acid bacteria (LAB) against clinical strains of H. pylori.Materials and Methods: Study of antibacterial effects of LAB against H. pylori strains included: evaluation of LAB effects as well as its cell-free supernatant (CFS) to reduce the number of H. pylori, and to examine the effects of CFS to inhibit the urease activity of H. pylori. The anti-adhesion effect of LAB on adherence of H. pylori to epithelial cell line was also evaluated.Results: Evaluation of the anti H. pylori effect of LAB depended on the strain of H. pylori and Lactobacillus. However, CFS of LAB reduced significantly the growth of all H. pylori strains. Also, urease activity of H. pylori strains was inhibited by CFS of LAB demonstrating that their organic acid may have a role in this inhibition. The significant anti-adhesion effect of LAB on adherence of H. pylori was also observed.Conclusion: Presence of LAB and/or their CFS can reduce the count of H. pylori, inhibit the urease activity of H. pylori, and reduce adhesion of H. pylori to epithelial cell line. This may be important for the impact of H. pylori colonization in the host stomach.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1118
1124
https://ijbms.mums.ac.ir/article_13811_2a966b3fe956e2077ead266d383870a7.pdf
dx.doi.org/10.22038/ijbms.2019.33321.7953
Association between cytokines and two circulating micro-RNAs and development of premature ventricular contractions-induced cardiomyopathy
Behshid
Ghadrdoost
Physiology Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
author
Nahid
Aboutaleb
Physiology Research Center, Physiology Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
author
Mahin
Nikoguftar Zarif
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
author
Mojdeh
Nakhlestani
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
author
Majid
Haghjoo
Cardiac Electrophysiology Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
author
Shahram
Samiee
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
author
text
article
2019
eng
Objective(s): Recent progress in understanding the pathogenesis of premature ventricular contraction (PVC)-induced cardiomyopathy (PIC) has suggested a key role for inflammation. The aim of this study was to evaluate the expression of messenger RNAs (mRNAs) and the protein production of interleukin-6 (IL-6), IL-10, tumor necrosis factor alpha (TNF-α) and interferon-γ (IFN-γ) and two circulating micro-RNAs related to inflammation and cardiovascular disease; miR-155 and miR-146.Materials and Methods: The study population was comprised 25 patients with PIC and 25 patients with normal left ventricular ejection fraction despite frequent PVCs. TNF-α, IL-6, IL10, and IFN-γ levels were evaluated in peripheral blood mononuclear cells (PBMCs) by flow cytometry and their mRNAs were assessed by real time PCR. We analyzed circulating levels of these cytokines by enzyme linked immunosorbent assay (ELISA). Two circulating micro-RNAs, miR-155 and miR-146a, were also investigated.Results: The flow cytometry findings showed that the median fluorescence intensity (MFI) of antibodies reacted with the IL-6 and TNF-α were higher in PIC group than the control group (P-value<0.001). In ELISA, the levels of IL-6 (P-value <0.001) and TNF-α (P-value <0.001) and in RT-PCR the relative expression levels of IL-6 (P-value <0.001) and TNF-α (P-value <0.001) were significantly higher in the PIC group. The relative expression levels of miR-155 and miR-146a were not significantly different between 2 groups (P-value>0.05).Conclusion: In our patients with PIC, there was an elevation in the expression levels of IL-6 and TNF-α in PBMCs. This finding may provide further insights into the inflammatory pathways involved in PIC.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1125
1131
https://ijbms.mums.ac.ir/article_13719_9482a9bebd585c647d40c0304020e98b.pdf
dx.doi.org/10.22038/ijbms.2019.36362.8662
Knockdown of mitofilin inhibits autophagy and facilitates starvation-induced apoptosis in HeLa cells
Mengli
Shen
Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China 325035
author
Li
Wang
Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China 325035
author
Lingyun
Kuang
Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China 325035
author
Danhui
Liu
Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China 325035
author
text
article
2019
eng
Objective(s): Mitofilin contributes to the maintenance of mitochondrial structure and functions. This study was undertaken to determine the mechanisms underlying its regulation of apoptosis. Materials and Methods: Mitofilin was knockdowned by specific short hairpin RNA (shRNA) and the stable HeLa cell clone was selected. The autophagy activity were assessed with LC3-II conversion and puncta formation by western blot and fluorescence imaging in starved and normal cultured HeLa cells. Autophagy flux was measured in the presence of NH4Cl. Wortmannin was used to inhibit autophagy. Cell viability and apoptosis were detected with cell counting kit-8 (CCK-8) and fluorescence-activated cell sorting (FACS) assay, respectively. Results: Mitofilin expression was down-regulated in starved HeLa cells. In established mitofilin stable knockdown cell lines, LC3-II conversion and puncta formation were detected, which are both hallmarks of autophagy, under both basal and starvation conditions. Mitofilin down-regulation decreased LC3-II conversion and puncta formation, which indicates that loss of mitofilin function inhibits both basal and starvation-induced autophagy activity. CCK-8 and FACS analysis confirmed mitofilin involvement in the regulation of cell survival since mitofilin down-regulation facilitated starvation-induced apoptosis in HeLa cells. Conclusion: Taken together, mitofilin is a potent regulator of autophagy and it may modulate cell survival through regulation of autophagy.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1132
1137
https://ijbms.mums.ac.ir/article_13598_70ad42f059a35f760e5dc1ba6acf99fe.pdf
dx.doi.org/10.22038/ijbms.2019.36173.8617
Colchicine-like β-acetamidoketones as inhibitors of microtubule polymerization: Design, synthesis and biological evaluation of in vitro anticancer activity
Ehsan
Karimikia
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
Javad
Behravan
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
Afshin
Zarghi
Department of Pharmaceutical Chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
Morteza
Ghandadi
Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Sina
Omid Malayeri
Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Razieh
Ghodsi
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2019
eng
Objective(s): In this study a series of novel colchicine-like β-acetamidoketones was designed and synthesized as potential tubulin inhibitorsMaterials and Methods: The cytotoxicity of the novel synthesized β-acetamidoketones was assessed against two cancerous cell lines including MCF-7 (human breast cancer cells) and A549 (adenocarcinomic human alveolar basal epithelial cells) employing the MTT test. Tubulin polymerization test was done by using a commercial kit (Tubulin Polymerization Assay Kit).Results: In general, the cytotoxicity activities were highly dependent on the aromatic substitution pattern of phenyl ring at β position of β-acetamidoketones. Based upon, compound 4f possessing the same structural elements of colchicine and chalcone 1, revealed the most cytotoxicity more than the other β-acetamidoketone against the cancerous cell lines and showed moderate antitubulin effect. The tubulin inhibitory effect of 4f, colchicine and chalcone 1 were consistent with their antiproliferative activities. Molecular docking studies of 4f, into the colchicine-binding site of tubulin exhibited possible mode of interaction between this compound and tubulin.Conclusion: The structure activity relationship (SAR) data attained showed that the presence of trimethoxy phenyl attached to carbonyl group of β-acetamidoketones and a methoxy group at para position of the other ring are essential for cytotoxic activity. In general, the cytotoxicity activities were highly dependent on the aromatic substitution pattern of phenyl ring at β position of β-acetamidoketones.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1138
1146
https://ijbms.mums.ac.ir/article_13596_eb66ad068ef309ce9c0ae9e519d791d3.pdf
dx.doi.org/10.22038/ijbms.2019.34760.8242
Does biofilm formation have different pathways in Staphylococcus aureus?
Ali
Shivaee
Microbial Biotechnology Research Center, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
author
behroz
sadeghi kalani
Microbial Biotechnology Research Center, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
author
Malihe
Talebi
Microbial Biotechnology Research Center, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
author
Davood
Darban-Sarokhalil
Microbial Biotechnology Research Center, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
author
text
article
2019
eng
Objective(s): Biofilm formation is one of the most important factors in the development of infections caused by Staphylococcus aureus. In this study, the expression levels of genes responsible for biofilm formation were studied in methicillin sensitive and methicillin resistant S. aureus.Materials and Methods: A total of 100 meticillin-resistant s.aureus (MRSA) and meticillin-sensetive s.aureus (MSSA) isolates were studied. Bacterial biofilm formation was evaluated phenotypically using microtiter plate method. Real-time PCR tests were conducted to determine the expression levels of genes involved in biofilm formation.Results: Quantitative biofilm formation test was repeated three times for each specimen. The prevalence of weak, medium, and strong biofilm producers were 16%, 49%, and 35%, respectively. In MSSA isolates, expression levels of ica genes increased compared to the fnbA, fnbB, clfA and clfB genes. These results were different in MRSA isolates, and ica genes showed a decreased gene expression levels compared to the aforementioned genes. Conclusion: Considering the results of this study, clf genes probably contribute to the same extent in both MRSA and MSSA isolates, and there is probably no significant difference in the role of these genes in these isolates. In addition, the results of this study indicated that MRSA may not use the conventional route for biofilm formation and may use independent pathways through Polysaccharide intercellular adhesion (PIA).
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1147
1152
https://ijbms.mums.ac.ir/article_13722_237d6ae3e23c97ea9a9c086c41202c22.pdf
dx.doi.org/10.22038/ijbms.2019.34888.8281
Investigation of histopathological and radiological effects of surfactant treatment in an experimental female rat model of lung contusion
Yasin
KESKİN
Beyhekim State Hospital, Department of Emergency Medicine, Konya, Turkey
author
Cihan
BEDEL
Health Science University Antalya Training and Research Hospital, Department of Emergency Medicine, Antalya, Turkey
author
Nesrin
Gökben Beceren
Süleyman Demirel University Faculty of Medicine, Department of Emergency Medicine, Isparta, Turkey
author
text
article
2019
eng
Objective(s): Pulmonary contusion (PC) is a clinical entity that often accompanies blunt traumas. We aimed to investigate the radiological and histopathological effects of surfactant treatment in an experimental rat model in which lung contusion was formed by blunt thoracic trauma.Materials and Methods: 50 female Sprague-Dawley rats were used. Five groups were formed randomly. In groups 2, 4, and 5 lung contusion was made by the drop-weight method after anesthesia. Intratracheal surfactant was administered in the 4th hr in groups 3 and 4 and in the 24th hr in groups 4 and 5. All rats were sacrificed and their lungs removed at 48 hr after contusion. Alveolar edema, congestion, hemorrhage, destruction, leukocyte infiltration, immune staining were examined histopathologically.Results: When the first thoracic CT scans were evaluated, we observed two rats with rib fractures and four rats with pneumothorax. 4 and 48 hr thoracic CT evaluation contusion and atelectasis showed no statistically significant decrease (P>0.05). After sacrifice of group 2, in macroscopic evaluation, there was a heterogeneous contusion and hemorrhagic appearance in the lungs of rats and less hemorrhagic appearance was observed in Groups 4 and 5 than in Group 2. In comparison of Immunohistopathological findings, surfactant treatment showed a statistically significant decrease in leukocyte infiltration scores (P=0.046). Immunohistopathologically, surfactant group had more staining but only statistically significant when compared to groups 4 and sham. (P=0.036).Conclusion: Surfactant treatment may be of significant benefit in lung contusion secondary to blunt chest trauma, and further prospective evidence of its efficacy in such disorders is needed.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1153
1157
https://ijbms.mums.ac.ir/article_13302_b638cf75da363853728c64af3f616ff2.pdf
dx.doi.org/10.22038/ijbms.2019.32357.8258
Filtered Kombucha tea ameliorates the leaky gut syndrome in young and old mice model of colitis
Nafiseh
Pakravan
Division of Immunology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
author
Fatemeh
Kkermanian
Department of Anatomy, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
author
Elaheh
Mahmoudi
Department of Mycology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
author
text
article
2019
eng
Objective(s): Zonula occludens proteins (ZO-1 and ZO-2) are important intracellular tight junction (TJ)-associated proteins that link the cell cytoskeleton to the trans-membrane TJ proteins. Destruction of TJ proteins is called the “leaky gut syndrome” and has been observed in some of the gastrointestinal diseases such as the inflammatory bowel disease (IBD). So, therapeutic approaches aim to restore the expression of TJ proteins and reduce intestinal permeability. Healing effect of Kombucha tea (KT), so-called long-life mushroom, on the gastrointestinal system, particularly its extraordinary healing effects on intestinal ulcers has been purported traditionally and rarely reported scientifically. This study aimed to investigate the therapeutic effect of filtered KT (fKT) in young and old mice model of colitis. Materials and Methods: Leaky gut was induced in two groups of young and old age using dextran sodium sulfate in drinking water for seven days. Then, fKT was administered to the mice affected by colitis and compared with the age-matched normal and untreated animals with colitis. Results: Survival rate of the fKT-treated young and old animals with colitis increased and weight loss decreased. Accordingly, digestive disorders characterized by bleeding and diarrhea were improved in fKT-treated mice. Molecular and histological examination indicated that expression of ZO-1 and ZO-2 was significantly improved in fKT-treated mice. Conclusion: Our results suggest KT as a promising therapeutic candidate to reduce intestinal permeability. Young animals with colitis showed more severe clinical signs and less survival rate than old mice with colitis, but this group responded better to fKT treatment than the old mice.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1158
1165
https://ijbms.mums.ac.ir/article_13599_e86f25fe423ad64822f49c83ebec4495.pdf
dx.doi.org/10.22038/ijbms.2019.36189.8622
Angiopoietin-like protein 8 (betatrophin) may inhibit hepatocellular carcinoma through suppressing of the Wnt signaling pathway
Nastaran
Monzavi
Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
author
Seyed Jalal
Zargar
Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
author
Nematollah
Gheibi
Cellular & Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
author
Mehdi
Azad
Department of Biotechnology, School of Paramedical Sciences, Qazvin University of Medical Sciences, Qazvin, Iran
author
Babak
Rahmani
Department of Biotechnology, School of Paramedical Sciences, Qazvin University of Medical Sciences, Qazvin, Iran
author
text
article
2019
eng
Objective(s): Hepatocellular carcinoma (HCC) is one of the leading fatal neoplasms and the most common primary liver malignancy worldwide. Peptide hormone ANGPTL8 (betatrophin) may act as an important regulator in HCC development through the Wnt/β-catenin pathway. We aimed to evaluate the effects of recombinant ANGPTL8 on Wnt/β-catenin signaling in human liver carcinoma cells (HepG2) and their viability. Materials and Methods: The expression of ANGPTL8 was conducted in the pET-21b-E. coli Bl21 (DE3) system and the produced peptide was purified. HepG2 cells were treated with different concentrations of ANGPTL8 (25, 50, 100, 150, 200, and 250 ng/ml) for 24, 48, and 72 hr. MTT assay was performed to detect the viability of treated cells, and apoptotic induction by ANGPTL8 was measured by flow cytometry assay. Finally, using qRT-PCR the mRNA levels of Wnt signaling modulators WIF-1 and β-catenin were evaluated in treated cells. Results: MTT assay showed that ANGPTL8 inhibits proliferation of HepG2 cells moderately in a time-independent manner. The highest concentration of the ANGPTL8, 250 ng/ml, reduced cell proliferation after 24, 48, and 72 hr similarly about 30%. In the same concentration of ANGPTL8, after 24 hr of treatment flow cytometry assay revealed a mild increase in early and late apoptosis up to 7.7 and 14.3%, respectively. The qRT-PCR showed that in a concentration-dependent and time-independent fashion, the expression of WIF-1 and β-catenin genes respectively increased and decreased significantly (P<0.05). Conclusion: Our findings suggest that ANGPTL8 may act as a moderate suppressor against HCC cell proliferation possibly via affecting Wnt signaling modulators.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1166
1171
https://ijbms.mums.ac.ir/article_13600_0b79ebe1484d2132d06f628c653cac17.pdf
dx.doi.org/10.22038/ijbms.2019.36612.8764
Idarubicin-bromelain combination sensitizes cancer cells to conventional chemotherapy
Abdullah
Taşkın
Nutrition and Dietetics Department, Faculty of Health Science, Harran University, Şanlıurfa, Turkey
author
Mehmet
Tarakçıoğlu
Department of Biochemistry, Medical Faculty, Gaziantep University, Gaziantep, Turkey
author
Hasan
Ulusal
Department of Biochemistry, Medical Faculty, Gaziantep University, Gaziantep, Turkey
author
Mustafa
Örkmez
Department of Biochemistry, Medical Faculty, Gaziantep University, Gaziantep, Turkey
author
Seyithan
Taysi
Department of Biochemistry, Medical Faculty, Gaziantep University, Gaziantep, Turkey
author
text
article
2019
eng
Objective(s): The primary cytotoxic effects of anticancer drugs like idarubicin, a chemotherapeutic agent, are not limited to neoplastic cells; they also produce similar effects in normal cells. In this study, we hypothesized that the combination of idarubicin-bromelain could make cancer cells more susceptible to cytotoxicity and genotoxicity.Materials and Methods: To test our hypothesis, the optimal concentrations of idarubicin and bromelain were combined and incubated in the HL-60 cancer cell line and normal human mononuclear leukocytes (PBMC) for 24, 48, and 72 hr. Cytotoxicity and genotoxicity were evaluated by measurement of ATP cell viability test, DNA damage, Caspase-3, Acridine orange/ethidium bromide (AO/EB), and DAPI fluorescent dyes in both cell types.Results: The combination of idarubicin-bromelain significantly reduced cell proliferation in the more potent HL-60 compared to PBMC in all incubation times (P<0.05). DNA damage and Caspase-3 levels (except for 24 hr) were also higher in the HL-60 cell line in comparison with PBMC and were statistically significant (P<0.05). The percentages of apoptotic images obtained by DAPI and AO / EB morphological examination were increased in both cells, depending on the combination dose. Conclusion: Based on these results, it can be concluded that idarubicin combined with bromelain produces more cytotoxic effects in low concentrations in comparison with when it was used per se in the HL-60 cells. Conversely, it was found that this combination in PBMC caused less cytotoxicity and less genotoxicity. Taken together, it can be said that this new combination makes cancer cells more sensitive to conventional therapy.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1172
1178
https://ijbms.mums.ac.ir/article_13602_01f028bd5a96d9e9ce1100bfc2dc538c.pdf
dx.doi.org/10.22038/ijbms.2019.37884.9003
Correlation between adolescent chronic emotional stress and incidence of adult cardiovascular disease in female rats
Monireh-Sadat
Mousavi
Laboratory of Neuro-Organic Chemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
author
Alireza
Imani
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
Sogol
Meknatkhah
Laboratory of Neuro-Organic Chemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
author
Gholamhossein
Riazi
Laboratory of Neuro-Organic Chemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
author
text
article
2019
eng
Objective(s): Association of adolescent emotional stress (ES) with the incidence of cardiovascular disease (CVD) at older age was investigated. Materials and Methods: 21 female rats were divided into three groups of 7 each; ES, foot-shock, and control. Chronic ES was induced by exposing the rats to witness foot-shock of their neighboring counterparts in the stress-box system in 5 successive days. 6 weeks after the last stress exposure, M-Mode echocardiographic assessment, qRT-PCR, and western blotting were performed in adult rats to determine the persistent effect of adolescent ES on cardiac performance and gene/protein expression levels of cardiac natriuretic peptide receptor 3 (NPR3) as a biomarker of CVD.Results: Interventricular septum thicknesses in diastole (IVSd) increased from 0.152±0.007 cm to 0.197±0.016 cm (P<0.05), left ventricular posterior wall thickness in diastole (LVPWd) significantly enlarged from 0.169±0.006 cm to 0.288±0.033 cm (P<0.01), left ventricular posterior wall thickness in systole (LVPWs) enlarged from 0.223±0.012 cm to 0.318±0.038 cm (P<0.05), left ventricular mass increased from 1.000±0.024 g to 1.283±0.084 g (P<0.01), and mean heart rate elevated from 229.42±6.57 bpm to 280.29±10.45 bpm (P<0.01). Moreover, ES significantly upregulated the expression levels of cardiac NPR3 gene (P<0.01) and protein (P<0.01).Conclusion: The incidence of adult CVD seemed to be increased under the influence of adolescent ES. Consequently, we suggest that mental healthcare during adolescence would be a critical factor for adult CVD prevention.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1179
1185
https://ijbms.mums.ac.ir/article_13791_4847a0c4ed140ec78c4aaccb883717f1.pdf
dx.doi.org/10.22038/ijbms.2019.32888.7857
The role of locus coeruleus nucleus TRPV1 receptors in the development and expression of morphine dependence
Iman
Fatemi
Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran
author
Zahra
Hadadianpour
Physiology-pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Farangis
Fatehi
Physiology-pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Ali
Shamsizadeh
Physiology-pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Jalal
Hasanshahi
Physiology-pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Mitra
Abbasifard
Department of Internal Medicine, Ali-Ebn Abitaleb Hospital, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Ayat
Kaeidi
Physiology-pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
text
article
2019
eng
Objective(s): This study investigated the role of locus coeruleus (LC) nucleus TRPV1 receptors (TRPV1r) in the expression and development of morphine physical dependence by intra-LC administration of AMG9810 (selective TRPV1r antagonist) in male Wistar rats. Materials and Methods: For assessing the development of morphine dependence, AMG9810 (0.03 and 0.3 mM in 10% DMSO, 0.2 µl; intra-LC microinjection) was administered before each morphine administration for seven continues days (once daily; 6, 16, 26, 36, 46, 56, and 66 mg/kg; sc). Furthermore, for evaluating the expression of morphine dependence, a single dose of AMG9810 (0.03 and 0.3 mM in 10% DMSO, 0.2 µl; intra-LC microinjection) was administered to morphine-dependent rats on day 8 of the experiment. Results: Obtained data demonstrated that co-administration of TRPV1r antagonist with morphine reduced the development of morphine withdrawal syndrome somatic signs induced by naloxone. Moreover, single intra-LC administration of TRPV1r antagonist on the final day of the examination period significantly decreased the expression of some signs of morphine withdrawal in rats. Conclusion: The results showed that LC TRPV1r might be participating in the expression and development of morphine dependence.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1186
1191
https://ijbms.mums.ac.ir/article_13796_108148e3aa7f1137fd254902a9052736.pdf
dx.doi.org/10.22038/ijbms.2019.35055.8338
Hydrogen sulfide improves vessel formation of the ischemic adductor muscle and wound healing in diabetic db/db mice
Guo-Guang
Wang
Department of Pathophysiology, Wannan Medical College, Wuhu, China
author
Wei
Li
Department of Pathophysiology, Wannan Medical College, Wuhu, China
author
text
article
2019
eng
Objective(s): It has been demonstrated that hydrogen sulfide plays a vital role in physiological and pathological processes such as regulating inflammation, oxidative stress, and vessel relaxation. The aim of the study was to explore the effect of hydrogen sulfide on angiogenesis in the ischemic adductor muscles of type 2 diabetic db/db mice and ischemic diabetic wound healing.Materials and Methods: The femoral arteries of diabetic db/db mice were isolated and ligated for preparation of ischemic hind limb model. Round incision was made on ischemic and non-ischemic limbs. The wounds were treated with sodium bisulfide (hydrogen sulfide donor). Real-time PCR and Western blotting were used to measure transcription of vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), platelet derived growth factor (PDGF), hypoxia inducible factor-1α (HIF-1α) and endothelial nitric oxide synthase (eNOS) and protein expression of VEGF, VEGF receptor (VEGFR) and PDGF, PDGF receptor (PDGFR), respectively. Angiogenesis and morphological changes in adductor muscles were observed.Results: Hydrogen sulfide significantly increased transcription of VEGF, EGF, PDGF, HIF-1α, eNOS and protein expression of VEGF, PDGF, and phosphorylated VEGFR and PDGFR. Treatment with hydrogen sulfide significantly improved ischemic wound healing and formation of granulation tissue, and increased the number of small vessels in the ischemic adductor muscles.Conclusion: Our data suggested that hydrogen sulfide attenuated injury of ischemic adductor muscle, and promoted the ischemic diabetic wound healing via modulating angiogenesis in type 2 diabetic db/db mice.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1192
1197
https://ijbms.mums.ac.ir/article_13307_71998b513dc2394fe812426e7c3bc308.pdf
dx.doi.org/10.22038/ijbms.2019.36551.8709
The effect of nanomicelle curcumin, sorafenib, and combination of the two on the cyclin D1 gene expression of the hepatocellular carcinoma cell line (HUH7)
sareh
Hosseini
Cancer Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
jamshidkhan
chamani
Department of Biology, Faculty of Sciences, Islamic Azad University-Mashhad Branch, Mashhad, Iran
author
Mohadeseh
Sinichi
Student Research Committee, School of Para Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Amir Mohammad
Bonakdar
Student Research Committee, School of Para Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Zeinolabedin
Azad
Department of Biology, Faculty of Sciences, Islamic Azad University-Mashhad Branch, Mashhad, Iran
author
Najmeh
Ahangari
Department of Modern Sciences & Technologies, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Hamid Reza
Rahimi
Vascular and Endovascular Surgery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2019
eng
Objective(s): Hepatocellular carcinoma (HCC) is one of the most significant health condition around the world. As the only curative therapies, liver transplantation and surgical resection are the clinical treatments of HCC. Due to the systemic toxicity and severe side effects of these treatments, it is vital to establish new therapeutic approaches. The present study aimed to compare cyclin D1 (CCN D1) gene expression in hepatocellular carcinoma cell line (HUH7) when it is treated with nanomicelle curcumin and sorafenib. The purpose was to identify toxicity risk and antioxidant activity of these drugs.Materials and Methods: The toxic dose (IC50) of nanomicelle curcumin and sorafenib were detected after treatment of HUH7 cell lines with different dose of mentioned agents followed by MTT assay. CCN D1 gene expression was evaluated using real-time PCR. Following the Tukey’s multiple comparison tests, statistical analysis is done through Student’s t-test or ANOVA.Results: The expression of the CCN D1 gene was statistically significant (P<0.001) at 289.31, 128 and 152.36 for sorafenib, nanomicelle curcumin and SNC (sorafenib-nanomicelle curcumin) respectively. The finding of this study revealed that, in comparison to sorafenib alone, the treatment of HUH7 with a nanomicelle curcumin IC50 dose, in combination with sorafenib, might down-regulate CCN D1 gene expression.Conclusion: The present research indicates that the treatment of the cell line with only nanomicelle curcumin results in the down-regulation of cyclin D1. To further decrease cyclin D1 expression, the co-delivery of curcumin and sorafenib appears to induce the apoptotic process. As a result, the effect of sorafenib cytotoxicity and CCN D1 gene expression decreases twofold.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1198
1202
https://ijbms.mums.ac.ir/article_13790_704462829d667bb3c859ba7e83ca3f10.pdf
dx.doi.org/10.22038/ijbms.2019.35808.8530
The protective effect of Liza klunzingeri protein hydrolysate on carbon tetrachloride-induced oxidative stress and toxicity in male rats
Sana
Rabiei
Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran
author
Masoud
Rezaie
Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran
author
Zahra
Abasian
Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
author
Mohammad
Khezri
Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran
author
Mehdi
Nikoo
Department of Pathobiology and Quality Control, Artemia and Aquaculture Research Institute, Urmia University, Urmia, Iran
author
Mahmoud
Rafieian-kopaei
Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
author
Maryam
Anjomshoaa
Department of Anatomical Sciences, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
author
text
article
2019
eng
Objective(s): Today, consumers are looking for food products providing health benefits in addition to meeting the basic nutritional needs of the body. This study aimed to evaluate the antioxidant and cytotoxic effects of Liza klunzingeri protein hydrolysate both in vivo and in vitro.Materials and Methods: Fish protein hydrolysate (FPH) was prepared using enzymatic hydrolysis with papain. In vitro antioxidant activity was assessed using five different antioxidant assays. The cytotoxic effect on 4T1 cell line was evaluated using the MTT assay. The distribution of the molecular weight of FPH was measured using HPLC. In the in vivo study, CCl4-exposed Wistar rats were orally treated with FPH (150, 300, and 600 mg/kg) or gallic acid (50 mg/kg) for 28 consecutive days.Results: Enzymatic hydrolysis gave hydrolysate rich in low molecular weight peptides (Conclusion: L. klunzingeri protein hydrolysate can be considered as a functional food to alleviate oxidative stress.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1203
1210
https://ijbms.mums.ac.ir/article_13793_691728bfaa118b0596a21a3f3967fd2f.pdf
dx.doi.org/10.22038/ijbms.2019.33201.7927
Gender associated effects of the ethanolic extracts of Chinese propolis on the hepatic transcriptome in ethanol-treated mice
Manhong
Ye
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu Province, China
author
Mengting
XU
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu Province, China
author
Mengmeng
Ding
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu Province, China
author
Chao
Ji
Fubiao Biotech Co, Ltd, Huai-an 211799, Jiangsu Province, China
author
Jian
Ji
Fubiao Biotech Co., Ltd., Huai-an 21799, Jiangsu Province, China
author
Fubiao
Ji
Fubiao Biotech Co., Ltd., Huai-an 21799, Jiangsu Province, China
author
Wanhong
Wei
Joint International Research Laboratory of Agricultural & Agri-Product Safety, Yangzhou University, Yangzhou 225009, Jiangsu Province, China
author
Shengmei
Yang
Joint International Research Laboratory of Agricultural & Agri-Product Safety, Yangzhou University, Yangzhou 225009, Jiangsu Province, China
author
Bin
Zhou
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu Province, China
author
text
article
2019
eng
Objective(s): The current study investigated the potential hepatoprotective effects of the ethanolic extracts of Chinese propolis (EECP) on ethanol-induced fatty liver in mice.Materials and Methods: C57BL/6J mice were orally gavaged with 50% ethanol alone or co-administrated with EECP at the dose of 0.2 ml/kg bodyweight for eight weeks. The dose for ethanol was 6 ml/kg bodyweight for the first two experimental weeks, and then increased to 8, 10, and 12 ml/kg bodyweight every two experimental weeks. Alterations in the hepatic transcriptome due to concomitant administration of EECP were investigated using RNA-Seq technique.Results: Our results showed that the main EECP-responsive genes were involved in lipid syntheses, which were significantly down-regulated in both female and male mice co-administrated with EECP. In female mice, these differentially expressed genes (DEGs) were mainly associated with fatty acid biosynthesis. While in male mice, these DEGs were mainly involved in the steroid metabolic process and cholesterol biosynthetic process. Despite the sex-associated responses in lipid metabolism, EECP also exerted other beneficial effects in female mice through modulation of the cytokine-cytokine receptor interaction pathway that helped explaining its hepato-protective effectiveness.Conclusion: Our findings indicated that the mechanism regarding the hepato-protective effects of EECP was gender-dependent, which is worthy of further investigation during the development of therapeutic interventions using EECP to reduce the adverse influences of ethanol.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1211
1217
https://ijbms.mums.ac.ir/article_13794_30bfddfbab6e4b61eef7f5ff04322719.pdf
dx.doi.org/10.22038/ijbms.2019.37348.8886
Centella asiatica (Gotu kola) ethanol extract up-regulates hippocampal brain-derived neurotrophic factor (BDNF), tyrosine kinase B (TrkB) and extracellular signal-regulated protein kinase 1/2 (ERK1/2) signaling in chronic electrical stress model in rats
Dwi Cahyani Ratna
Sari
Department of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
author
Nur
Arfian
Department of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
author
Untung
Tranggono
Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
author
Wiwit Ananda
Setyaningsih
Department of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
author
Muhammad Mansyur
Romi
Department of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
author
Noriaki
Emoto
Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Japan
author
text
article
2019
eng
Objective(s): Impairment of hippocampus function as a center for memory processing occurs due to stress. Centella asiatica L. (Gotu kola) is known to improve memory, intelligence, and neural protection although the precise mechanism is not well understood. This study aimed to investigate the effects of ethanol extracts of C. asiatica toward MAPK expression as down-stream signaling of brain-derived neurotrophic factor (BDNF). Materials and Methods: We performed a chronic electrical stress model on 20 male Sprague Dawley rats (2 months-old, 180–200 g). Rats were divided into four groups: normal control group (Control) which received distilled water, and three treatment groups receiving oral Gotu kola ethanol extracts in oral doses of 150 mg/kg BW (CeA150), 300 mg/kg BW (CeA300), and 600 mg/kg BW (CeA600) over four weeks. Memory acquisition was assessed with Morris water maze. Hippocampus was harvested, then extracted for protein and RNA analysis. MAPK proteins (p38, ERK1/2, JNK) were measured using Western blot, meanwhile, BDNF and TrkB receptor were analyzed with real-time PCR (RT-PCR). Results: CeA600 group revealed improvement of memory performance as shown by reduction in time and distance parameters compared to control during escape latency test. This finding associated with significant elevation of hippocampal BDNF protein and mRNA level with up-regulation of TrkB mRNA expression in CeA600 group compared to control. Western-blot analysis showed significant up-regulation of ERK1/2 protein level in CeA600 group (P<0.05) compare to control. Conclusion: BDNF signaling through TrkB and ERK1/2 pathway contributes significantly to amelioration of memory performance after C. asiatica treatment in electrical stress model.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
1218
1224
https://ijbms.mums.ac.ir/article_13721_1d5460753edd4b648f3dafe0a591605e.pdf
dx.doi.org/10.22038/ijbms.2019.29012.7002
Retracted: Time course changes of oxidative stress and inflammation in hyperoxia-induced acute lung injury in rats
text
article
2019
eng
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
22
v.
10
no.
2019
https://ijbms.mums.ac.ir/article_13852_4fa2e13056ff3d4af0f5b1fc44686e1d.pdf
dx.doi.org/10.22038/ijbms.2019.13852