Flavonoid-rich foods (FRF): A promising nutraceutical approach against lifespan-shortening diseases
Alhamzah Hasan
Waheed Janabi
State Key Laboratory of Natural Medicines, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, Jiangsu Province, PR China
author
Asghar Ali
Kamboh
Department of Veterinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam 70060, Pakistan
author
Muhammad
Saeed
Faculty of Animal Production and Technology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur 6300, Pakistan
author
Lu
Xiaoyu
State Key Laboratory of Natural Medicines, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, Jiangsu Province, PR China
author
Jannat
BiBi
Department of Physical Education, Shaanxi Normal University, Xian, Shaanxi Province, P.R China
author
Fatima
Majeed
School of Public Health, Nanjing Medical University, Jiangsu Province, 211166, P.R China
author
Muhammad
Naveed
School of Pharmacy, Nanjing Medical University, Jiangsu Province, 211166, P.R China
author
Muhammad
Jameel Mughal
Faculty of Health Sciences, University of Macau, Avenida de UniversidadeTaipa, Macau
author
Nazar Ali
Korejo
Department of Veterinary Medicine, Faculty of Animal Husbandry and Veterinary Science, Sindh Agriculture University, Tandojam 70060, Pakistan
author
Rubina
Kamboh
Liaquat University of Medical and Health Sciences, Jamshoro, Pakistan
author
Mahmoud
Alagawany
Departmentof Poultry, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt
author
Lv
Huixia
State Key Laboratory of Natural Medicines, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, Jiangsu Province, PR China
author
text
article
2020
eng
It is well documented that life expectancy in developed countries at birth is going to increase from the 20th century. However, regrettably, a potential decline in life expectancy has been proposed for these nations in the 21st century due to a rapid upsurge in the prevalence of fatal degenerative diseases like cardiovascular diseases (CVD), cancer and diabetes. Collectively, these three diseases accounted for 65% of all deaths in urbanized societies and considered as a dynamic issue for shortening the genetically determined lifespan through increased mortalities, morbidities, disabilities, immense sufferings, and premature aging. These fatal degenerative diseases and premature aging are closely associated with oxidative stress produced by the free radicals in the body. In epidemiologic studies, flavonoid-rich foods (FRF) like fruits, vegetables, and beverages have been associated as protective agents against these diseases. These also have been observed for their geroprotective effects and help in preventing the premature aging and the deterioration of brain function, which is related to Alzheimer’s disease and dementia. In this review, we presented a comprehensive overview of the FRF for their potential role against lifespan-shortening complications, i.e., CVD, cancer, and diabetes. We also had drawn the future perspective and dietary guidelines to reduce the fatal diseases burden in urban populations.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
140
153
https://ijbms.mums.ac.ir/article_14327_84f5823cad59043095e379aaec9f7c45.pdf
dx.doi.org/10.22038/ijbms.2019.35125.8353
Looking for immortality: Review of phytotherapy for stem cell senescence
Hourieh
Tousian
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Bibi Marjan
Razavi
Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
Hossein
Hosseinzadeh
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2020
eng
Objective(s): In this paper, we discussed natural agents with protective effects against stem cell senescence. Different complications have been observed due to stem cell senescence and the most important of them is “Aging”. Senescent cells have not normal function and their secretary inflammatory factors induce chronic inflammation in body which causes different pathologies. Stem cell senescence also has been investigated in different diseases or as drug adverse effects.Materials and Methods: We searched databases such as Embase, Pubmed and Web of Science with keywords “stem cell”, “progenitor cell”, “satellite”, “senescence” and excluded keywords “cancer”, “tumor”, “malignancy” and “carcinoma” without time limitation until May 2019. Among them we chose 52 articles that have investigated protective effects of natural agents (extracts or molecules) against cellular senescence in different kind of adult stem cells.Results: Most of these studies were in endothelial progenitor cells, hematopoietic stem cells, mesenchymal stem cells, adipose-derived stem cells and few were about other kinds of stem cells. Most studied agents were resveratrol and ginseng which are also commercially available as supplement. Most protective molecular targets were telomerase and anti-oxidant enzymes to preserve genome integrity and reduce senescence-inducing signals.Conclusion: Due to the safe and long history of herbal usage in clinic, phytotherapy can be used for preventing stem cell senescence and their related complication. Resveratrol and ginseng can be the first choice for this aim due to their protective mechanisms in various kinds of stem cells and their long term clinical usage.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
154
166
https://ijbms.mums.ac.ir/article_14292_e411bf0344a07b03f215cdc3a635dc1e.pdf
dx.doi.org/10.22038/ijbms.2019.40223.9522
Effect of gallic acid on electrophysiological properties and ventricular arrhythmia following chemical-induced arrhythmia in rat
Ghaidafeh
Akbari
Department of Physiology, Persian Gulf Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
author
Mahin
Dianat
Department of Physiology, Persian Gulf Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
author
Mohammad
Badavi
Department of Physiology, Persian Gulf Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
author
text
article
2020
eng
Objective(s): Ventricular arrhythmias including ventricular tachycardia (VT) and ventricular fibrillation (VF) are the most important causes of mortality rate. Gallic acid (GA) has beneficial effects on cardiovascular diseases. The aim of this study was to evaluate the effects of GA on electrophysiological parameters such as QRS complex, heart rate (HR), PR interval parameters, and ventricular arrhythmia following chemical induction in rat.Materials and Methods: Seventy-two male rats were divided into 9 groups (n=8). Chronic groups pretreated by GA (10, 30, and 50 mg/kg, orally) and normal saline (N/S, 1 ml/kg, orally) for 10 days. At the start of the experiments (the first day) and on the final day of the experiments (tenth day), the electrocardiogram (lead II) was recorded. At acute group, GA (50 mg/kg), and anti-arrhythmic drugs such as propranolol, amiodarone, and verapamil injected via intravenous (IV). Then, arrhythmia induced by a CaCl2 2.5% solution (140 mg/kg, IV). Afterward, percentage of premature ventricular beats (PVB), VF, and VT were recorded at 1, and 3 min. Results: These findings showed that chronic and acute doses of GA have positive inotropic and anti-dysrhythmic effects by significant reduction of PVB, VT and VF on comparison with the control group. These actions are comparable to anti-arrhythmic drugs such as quinidine, propranolol, amiodarone, and verapamil. GA has not significant effect on chronotropic and dromotropic properties.Conclusion: Findings showed that GA has antiarrhythmic, and inotropic characteristics that suggested GA has effective for mild congestive heart failure, and cardiovascular disorders patients which susceptible to incidence of arrhythmias.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
167
172
https://ijbms.mums.ac.ir/article_14141_77f738a7e0be06ed014109574362c450.pdf
dx.doi.org/10.22038/ijbms.2019.33296.7948
Online analysis of local field potentials for seizure detection in freely moving rats
Meysam
Zare
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Milad
Nazari
Department of Technology, Electrical Engineering, Sharif University, Tehran
author
Amir
Shojaei
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Mohammad Reza
Raoufy
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Javad
Mirnajafi-Zadeh
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
text
article
2020
eng
Objective(s): Seizure detection during online recording of electrophysiological parameters is very important in epileptic patients. In the present study, online analysis of field potential recordings was used for detecting spontaneous seizures in epileptic animals.Materials and Methods: Epilepsy was induced in rats by pilocarpine injection. During the chronic period of the pilocarpine model, local field potential (LFP) recording was run for at least 24 hr. At the same time, video monitoring of the animals was done to determine the real time of seizure occurrence. Both power and sample entropy of LFP were used for online analysis.Results: Obtained results showed that changes in LFP power are a better index for seizure detection. In addition, when we used one hundred consecutive epochs (each epoch equals 10 ms) of LFP for data analysis, the best detection was achieved.Conclusion: It may be suggested that power is a suitable parameter for online analysis of LFP in order to detect the spontaneous seizures correctly.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
173
177
https://ijbms.mums.ac.ir/article_14199_27dc6ca8d53e8495902d44b73ec3743d.pdf
dx.doi.org/10.22038/ijbms.2019.38722.9183
Microbiome alterations are related to an imbalance of immune response and bacterial translocation in BDL-rats
Natali
Vega-Magaña
Instituto de Investigación en Enfermedades Crónico Degenerativas, Departamento de Biología Molecular y Genómica. Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara. CP 44340, Guadalajara, Jalisco, México
author
Antonio
Galiana
FISABIO Fundación para el fomento de la Investigación Sanitaria y Biomédica de la comunidad de Valencia. CP 46015, España
author
Luis Felipe
Felipe Jave-Suárez
División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. CP 44340, Guadalajara, Jalisco, México
author
Leonel
Garcia-Benavides
Departamento de Ciencias Biomédicas, Centro Universitario de Tonalá, Universidad de Guadalajara. CP 45425, Tonalá, Jalisco, México
author
Susana
Del Toro-Arreola
Instituto de Investigación en Enfermedades Crónico Degenerativas, Departamento de Biología Molecular y Genómica. Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara. CP 44340, Guadalajara, Jalisco, México
author
Jaime Federico
Andrade-Villanueva
Instituto de Investigación en Inmunodeficiencias y VIH. Departamento de Clínicas Médicas. Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara. CP 44340, Guadalajara, Jalisco, México
author
Luz Alicia
González-Hernández
Instituto de Investigación en Inmunodeficiencias y VIH. Departamento de Clínicas Médicas. Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara. CP 44340, Guadalajara, Jalisco, México
author
Rosa
Cremades
Departamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara. CP 44340, Guadalajara, Jalisco, México
author
Adriana
Aguilar-Lemarroy
División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. CP 44340, Guadalajara, Jalisco, México
author
María Guadalupe
Flores-Miramontes
División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. CP 44340, Guadalajara, Jalisco, México
author
Jesse
Haramati
Laboratorio de Inmunología, Departamento de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara. CP 44600, Guadalajara, Jalisco, México
author
Jesus
Meza-Arroyo
Departamento de Ciencias de la Salud, Centro Universitario de los Valles, Universidad de Guadalajara. CP 46600, Ameca, Jalisco, México
author
Miriam Ruth
Bueno-Topete
Instituto de Investigación en Enfermedades Crónico Degenerativas, Departamento de Biología Molecular y Genómica. Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara. CP 44340, Guadalajara, Jalisco, México
author
text
article
2020
eng
Objective(s): Bacterial translocation in patients with cirrhosis is an important triggering factor for infections and mortality. In the bile duct ligation (BDL) model, crucial players of bacterial translocation are still unknown. This study aims to determine the interrelation between microbiome composition in the colon, mesenteric lymph nodes, and liver, as well as the local inflammatory microenvironment in the BDL model.Materials and Methods: Liver damage was assayed by Masson trichrome staining, and hepatic enzymes. The diversity of microbiota in colon stools, mesenteric lymph nodes, and liver was determined by 16S rRNA pyrosequencing. Cytokine expression in mesenteric lymph nodes was analyzed by qRT-PCR.Results: Our results show that Proteobacteria was the predominant phylum found to translocate to mesenteric lymph nodes and liver in cirrhotic rats. Bile duct ligation induces a drastic intestinal dysbiosis, revealed by an increased relative abundance of Sarcina, Clostridium, Helicobacter, Turicibacter, and Streptococcus genera. However, beneficial bacteria, such as Lactobacillus, Prevotella and Ruminococcus were found to be notably decreased in BDL groups. Mesenteric pro-inflammatory (TNF-α, IL-1β, IL-6, TLR-4) and regulatory (TGF-β, Foxp3, and IL-10) molecules at 30 days post-BDL were significantly increased. Conversely, TGF-β and Foxp3 were significantly augmented at 8 days post-BDL. Conclusion: Dysbiosis in the colon and mesenteric lymph nodes is linked to an imbalance in the immune response; therefore, this may be an important trigger for bacterial translocation in the BDL model.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
178
185
https://ijbms.mums.ac.ir/article_14289_0d337aafd3a77702fc025a5e821fbc1d.pdf
dx.doi.org/10.22038/ijbms.2019.36487.8753
The metabolome profiling of obese and non-obese individuals: Metabolically healthy obese and unhealthy non-obese paradox
Saeed
Chashmniam
Department of Chemistry, Sharif University of Technology, Tehran, Iran
author
Nahid
Hashemi Madani
Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran
author
BiBi Fatemeh
Nobakht Mothlagh Ghoochani
Chemical Injuries Research Center, Systems Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
author
Nahid
Safari-Alighiarloo
Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran
author
Mohammad E.
Khamseh
Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran
author
text
article
2020
eng
Objective(s): The molecular basis of “metabolically healthy obese” and “metabolically unhealthy non-obese” phenotypes is not fully understood. Our objective was to identify metabolite patterns differing in obese (metabolically healthy vs unhealthy (MHO vs MUHO)) and non-obese (metabolically healthy vs unhealthy (MHNO vs MUHNO)) individuals. Materials and Methods: This case-control study was performed on 86 subjects stratified into four groups using anthropometric and clinical measurements: MHO (21), MUHO (21), MHNO (22), and MUHNO (22). Serum metabolites were profiled using nuclear magnetic resonance (NMR). Multivariate analysis was applied to uncover discriminant metabolites, and enrichment analysis was performed to identify underlying pathways.Results: Significantly higher levels of glutamine, asparagine, alanine, L-glutathione reduced, 2-aminobutyrate, taurine, betaine, and choline, and lower level of D-sphingosine were observed in MHO group compared with MUHO. In comparison of MHNO and MUHNO groups, significantly lower levels of alanine, glycine, glutamine, histidine, L-glutathione reduced, and betaine, and higher levels of isoleucine, L-proline, cholic acid, and carnitine appeared in MUHNO individuals. Moreover, significantly affected pathways included amino acid metabolism, urea cycle and ammonia recycling in MUHO subjects and glutathione metabolism, amino acid metabolism, and ammonia recycling in MUHNO members. Conclusion: Literature review helped us to hint that altered levels of most metabolites might associate to insulin sensitivity and insulin resistance in MHO and MUHNO individuals, respectively. Besides, abnormal amino acid metabolism and ammonia recycling involved in unhealthy phenotypes (MUHO, MUHNO) might be associated with insulin resistance.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
186
194
https://ijbms.mums.ac.ir/article_14337_86b3c60e1c2ec3465951463a112fe100.pdf
dx.doi.org/10.22038/ijbms.2019.37885.9004
Purification and characterization of a novel type of neurotoxic peptides from the venom of the Iranian scorpion Hemiscorpius lepturus
Mahboob
Maleki
Department of Biochemistry, Fars Science and Research Branch, Islamic Azad University, Fars, Iran
author
Naser
Mohammadpour Dounighi
Department of Human Vaccine and Serum, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
author
text
article
2020
eng
Objective(s): Scorpion venom has toxic effects on mammals, insects and crustaceans. Toxicogenic peptides are major contributors to the scorpion venom, which make it toxic. The Hemiscorpius lepturus (H. lepturus) is one of the most common scorpion bites agent, and responsible for 95% of scorpion bite deaths cases in Iran.Materials and Methods: In this project, we fractionated the H. lepturus scorpion venom and analyzed toxic fractions of the venom. The crude venom of H. lepturus was dialyzed against distilled water and then the soluble part of the venom was isolated from the non-soluble (mucoproteins) part of the venom and loaded onto the Sephadex G-50 gel filtration column, then after determining the toxicity of the obtained fractions (fractions toxicity were detected in mice by IV injection), the resulting toxic fraction was purified with three stages of ion-exchange chromatography (anion and cationic) and RP-HPLC. The purity of the fractions was verified by SDS-PAGE electrophoreses.Results: The LD50 of H. lepturus venom was 177.01 µg/mouse. The crude venom had 7 detectable bands with molecular weights of 10-100 KDa and one band less than 10 KDa. Finally, after the different stages of chromatography, two HL2153 and HL2155 peaks were obtained from the RP-HPLC, which were depicted single bands and high purity. The electrophoretic analysis showed molecular weight 4874 Da for HL2153 and 5107 Da for HL2155 toxins.Conclusion: It is concluded that H. lepturus venom contains two HL2153 and HL2155 toxins with a relatively similar molecular weight and similar electrical charge 4874 and 5107 Da, respectively.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
195
201
https://ijbms.mums.ac.ir/article_14197_49dd801ae40790b8dc59019a193dea88.pdf
dx.doi.org/10.22038/ijbms.2019.37910.9025
Change of Nurr1 expression in mouse hippocampal CA3 region following excitotoxic neuronal damage
Choong-Hyun
Lee
Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan 31116, Republic of Korea
author
text
article
2020
eng
Objective(s): Nuclear receptor-related protein 1 (Nurr1), one of immediate-early genes, is a member of orphan nuclear receptor family. The aim of this study was to investigate the time-dependent change of Nurr1 protein expression in the mouse hippocampal CA3 region following kainic acid (KA)-induced excitotoxic neuronal damage.Materials and Methods: Male ICR mice were used as experimental animals, and 30 mg/kg KA was administered intraperitoneally. To confirm the KA-induced neuronal damage in the hippocampal CA3 region, Fluoro-Jade B histofluorescence staining was performed. In addition, the time-dependent change of Nurr1 protein expression was also examined using immunohistochemistry and western blot analysis. Results: Marked neuronal damage was observed in the hippocampal CA3 region at 24 hr after KA injection. In addition, both Nurr1 immunoreactivity and protein level were significantly increased at 6 hr and 12 hr after KA injection, and then decreased at 24 hr after KA injection.Conclusion: This result indicates that KA-induced alteration of Nurr1 protein expression may be associated with the neuronal degeneration in the hippocampal CA3 region after KA injection.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
202
205
https://ijbms.mums.ac.ir/article_14178_08686982aa6afdca138556a24bce065b.pdf
dx.doi.org/10.22038/ijbms.2019.38712.9177
Protection of renal damage by HMG-CoA inhibitors: A comparative study between atorvastatin and rosuvastatin
Maryam
Jabarpour
Department of Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
author
Nadereh
Rashtchizadeh
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
Amir
Ghorbani Haghjo
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
Hassan
Argani
Urology and Nephrology Research Center, Beheshti University of Medical Sciences, Tehran, Iran
author
Mahboob
Nemati
Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
Siavoush
Dastmalchi
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
Leila
Roshangar
Stem Cell Research Center, Tabriz University of Medical Sciences. Tabriz, Iran
author
Masoumeh
Ranjbarzadhag
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
Mehran
Mesgari-Abbasi
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
Nasrin
Bargahi
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
Davoud
Sanajou
Department of Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
author
text
article
2020
eng
Objective(s): Hypercholesterolemia is a common metabolic disorder in developing and developed countries and is associated with the increased rates of chronic kidney disease (CKD). Statin therapy could reduce cholesterol synthesis as well as progression of CKD. Diversity between statins causes variety in pharmacokinetics and pharmacodynamics and also their pleiotropic effects. In the present investigation we aimed to evaluate the protective potentials of both atorvastatin (Ator) (as lipid-soluble statin) and rosuvastatin (Ros) (as water-soluble statin) against renal histopathological damages in the high cholesterol diet induced hypercholesterolemic rats (HCDIHR).Materials and Methods: Serum lipid profile, oxidized low density lipoprotein (OX-LDL), malondialdehyde (MDA), urea and creatinine levels, as well as renal histopathology were evaluated.Results: While Ros acted better than Ator to reduce serum low density lipoprotein cholesterol (LDL-C) (P<0.01), atherogenic index (AI) (P<0.01), MDA (P<0.01), and OX-LDL (P<0.01); no significant differences were noted in their cholesterol (P=0.72), triglyceride (TG) (P=0.79), and very low density lipoprotein cholesterol lowering (VLDL-C) (P=0.79) and high density lipoprotein cholesterol elevating effects (HDL-C) (P=0.72). Ator was more effective to reduce renal histopathologic indices compared to Ros, including accumulation of lipid droplet, glomerular foam cells, mesangial cell proliferation, renal hemorrhage, and tubulointerstitial damages in the kidneys of diet induced hypercholesterolemic rats.Conclusion: The findings underline that the lipophilic Ator may performs better than Ros in attenuating renal damages in HCDIHR.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
206
213
https://ijbms.mums.ac.ir/article_14336_7c2ad291819f4a9cee288e606f664dbd.pdf
dx.doi.org/10.22038/ijbms.2019.38239.9080
The effect of human wharton’s jelly-derived mesenchymal stem cells on MC4R, NPY, and LEPR gene expression levels in rats with streptozotocin-induced diabetes
Fatemeh
Sabet Sarvestani
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
author
Mohammad Ali
Zare
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
author
Forough
saki
Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
author
Farhad
Koohpeyma
Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
author
Ismail H.
al-Abdullah
Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, Beckman Research Institute of City of Hope, Duarte, USA
author
Negar
Azarpira
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
author
text
article
2020
eng
Objective(s): Type 1 diabetes (T1D) is an autoimmune disease resulting from inflammatory destruction of islets β-cells. Nowadays, progress in cell therapy, especially mesenchymal stem cells (MSCs) proposes numerous potential remedies for T1D. We aimed to investigate the combination therapeutic effect of these cells with insulin and metformin on neuropeptide Y, melanocortin-4 receptor, and leptin receptor genes expression in TID. Materials and Methods: One hundreds male rats were randomly divided into seven groups: the control, diabetes, insulin (Ins.), insulin+metformin (Ins.Met.), Wharton’s Jelly-derived MSCs (WJ-MSCs), insulin+metformin+WJ-MSCs (Ins.Met.MSCs), and insulin+WJ-MSCs (Ins.MSCs). Treatment was performed from the first day after diagnosis as diabetes. Groups of the recipient WJ-MSCs were intraportally injected with 2× 10⁶ MSCs/kg at the 7th and 28th days of study. Fasting blood sugar was monitored and tissues and genes analysis were performed.Results: The blood glucose levels were slightly decreased in all treatment groups within 20th and 45th days compared to the diabetic group. The C-peptide level enhanced in these groups compared to the diabetic group, but this increment in Ins.MSCs group on the 45th days was higher than other groups. The expression level of melanocortin-4 receptor and leptin receptor genes meaningfully up-regulated in the treatment groups, while the expression of neuropeptide Y significantly down-regulated in the treatment group on both times of study. Conclusion: Our data exhibit that infusion of MSCs and its combination therapy with insulin might ameliorate diabetes signs by changing the amount of leptin and subsequent changes in the expression of neuropeptide Y and melanocortin-4 receptor.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
214
223
https://ijbms.mums.ac.ir/article_14331_1c79d3ec273192907589aca7b7ca27de.pdf
dx.doi.org/10.22038/ijbms.2019.39582.9387
Pharmacological and computational evaluation of Sapodilla and its constituents for therapeutic potential in hyperactive gastrointestinal disorders
Muhammad Bilal
Riaz
Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad
author
Arif-ullah
Khan
Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad
author
Neelam Gul
Qazi
Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad
author
text
article
2020
eng
Objective(s): This study was designed to investigate various gastrointestinal effects of Manilkara zapota (Sapodilla), exploring its anti-diarrheal, anti-secretary, anti-spasmodic, anti-ulcer and anti-motility potential.Materials and Methods: Antidiarrheal and anti-secretary activities were investigated using castor oil induced diarrhea and castor oil induced fluid accumulation. Isolated rabbit jejunum tissues (antispasmodic) were employed for in vitro experiments. Antiulcer, antimotility and molecular docking were performed using ethanol-HCl induced ulcer assay, charcoal meal transit time and Auto Doc Vina.Results: Mz.Cr exhibited protection against castor oil-induced diarrhea (P<0.05 vs. saline group) and dose-dependently inhibited intestinal fluid secretions (P<0.001 vs. castor oil group). Mz.Cr caused relaxation of spontaneous and K+ (80 Mm)-induced contractions with EC50 values of 0.11mg/ml (0.08-0.1, n=4) and 0.16 mg/ml (0.09-0.2, n=4) respectively (*P<0.05**P<0.01 ***P<0.001). It showed protective effect against gastric ulcers induced by ethanol-HCl (P<0.001 vs. saline group). Mz.Cr reduced distance travelled by charcoal meal (P<0.001 vs. saline group). Plant constituents: caffeoylquinic acid and methyl 4-O-galloylchlorogenate showed high binding affinities (E-value≥-6.5 Kcal/mol) against histaminergic H2 receptors, H+/K+ ATPase pump and voltage gated L-type calcium channels, while possesses moderate affinities (E-value≥8 Kcal/mol) against histaminergic H1, muscarinic M1, M3 and mu-opioid, whereas lower affinities (E-value≥9.5 Kcal/mol) vs. calmodulin, adrenergic α1, phosphodiesterase enzyme and dopaminergic D2 receptors. Lupeol-3-acetate and β-amyrin-3-(3’-dimethyl) butyrate observed weak affinities.Conclusion: In present study, M. zapota is reported to exhibits anti-diarrheal, anti-secretory, anti-spasmodic, anti-motility, anti-ulcer effects and computational binding affinities against gastrointestinal targets.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
224
235
https://ijbms.mums.ac.ir/article_14287_dbc8a00e6a98d891a701bd8cb71e017c.pdf
dx.doi.org/10.22038/ijbms.2019.35595.8488
Administration of sodium hydrosulfide reduces remote organ injury by an anti-oxidant mechanism in a rat model of varicocele
Keivan
Lorian
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
Mehri
Kadkhodaee
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
Farzaneh
Kianian
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
Arash
Abdi
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
Behjat
Seifi
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
text
article
2020
eng
Objective(s): Infertility in varicocele may have an adverse outcome on the future life of an infertile male. This study was designed to investigate whether varicocele affects remote organs, including the kidney, liver, and brain. We have also evaluated the protective effects of NaHS administration on the structure and function of these organs.Materials and Methods: Thirty-six rats were randomly assigned to 3 experimental groups: 1) Sham, 2) Varicocele, and 3) Varicocele + sodium hydrosulfide. Varicocele was induced via partial ligation of the left renal veins. Animals in the Varicocele + sodium hydrogen sulfide group received 30 µmol/l NaHS in drinking water for 56 days. On the 57th day of the treatment, blood samples, as well as kidney, liver, and brain tissues, were collected to assess kidney and liver functions, measurement of oxidative stress markers, and histological changes. For evaluation of sperm parameters caudal epididymis was used. The behavioral tests were performed to evaluate the animal’s anxiety-related behaviors. Results: Varicocele caused significant decrease in sperm parameters (motility and viability) and superoxide dismutase activity in the kidney, liver, and brain tissue. Anxiety-related parameters decreased in varicocele. Moreover, varicocele resulted in a significant increase in malondialdehyde levels in the kidney, liver and brain tissue, and liver function enzymes. Varicocele did not alter kidney function parameters. The administration of NaHS improves the above parameters.Conclusion: This study showed that notice to remote organs such as the liver and brain beside reproductive organs in varicocele is important. The administration of NaHS improved remote organ injury in varicocele via its anti-oxidant mechanism.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
236
243
https://ijbms.mums.ac.ir/article_14291_da9401d1ddfa96f916ada6077ddb5f03.pdf
dx.doi.org/10.22038/ijbms.2019.39727.9412
The effects of interval aerobic training on mesenchymal biomarker gene expression, the rate of tumor volume, and cachexia in mice with breast cancer
samira
gholamian
Department of Exercise Physiology (Biochemistry and Metabolism), Faculty of Sports Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
author
Seyyed Reza
Attarzadeh Hosseini
Department of Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
author
Amir
Rashidlamir
Department of Exercise Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
author
Hamid
Aghaalinjad
Department of Sports Sciences, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
author
text
article
2020
eng
Objective(s): It seems that regular exercise can have inhibitory effects on the progression of breast cancer. This study, therefore, aimed to investigate the influences of interval aerobic training on mesenchymal biomarker gene expression, muscle cachexia, and tumor volume changes in mice with breast cancer.Materials and Methods: Thirty-two female Balb/c mice were allocated to four groups: Exercise Tumor Exercise, Rest Tumor Rest (Control), Rest Tumor Exercise, and Exercise Tumor Rest. Interval aerobic training was done 6 weeks before and 4 weeks after tumor induction. Weight test and inverted screen test were carried out as muscle function tests. Data were analyzed using one-way ANOVA and HSD post hoc.Results: The results showed a significant decrease in gene expressions of Twist, Vimentin, and TGF-β in Exercise Tumor Exercise group in comparison with the Control group (P<0.05). Remarkable reduction of the rate of tumor volume was also observed in two training groups (Rest Tumor Exercise, Exercise Tumor Exercise) compared with the control group. According to function tests’ results, muscle functions were diminished due to cancer, but interval aerobic training can keep muscles in a normally-functioning state in cancer (P<0.05).Conclusion: Considering final results, a period of interval aerobic training can be used not only as a prevention method, but also help cancer treatment and impede cachexia by tumor volume reduction, decrease mesenchymal biomarker gene expression, and increase muscle strength functions.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
244
250
https://ijbms.mums.ac.ir/article_14334_802bf048244279f1b5f0361cf9592f76.pdf
dx.doi.org/10.22038/ijbms.2019.39535.9375
Therapeutic effect of psoralen on muscle atrophy induced by tumor necrosis factor-α
Xin-Feng
Lin
Intensive Care Unit, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China
author
Qi-Long
Jiang
Department of Spleen-Stomach, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China
author
Zhi-Long
Peng
The First Clinical School, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China
author
Yi-Le
Ning
The First Clinical School, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China
author
Yuan-Yuan
Luo
Intensive Care Unit, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China
author
Fu
Zhao
Intensive Care Unit, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China
author
Xian
Peng
The First Clinical School, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China
author
Wei-Tao
Chen
Intensive Care Unit, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China
author
text
article
2020
eng
Objective(s): To observe and determine the effect and mechanism of psoralen on tumor necrosis factor-α (TNF-α)-induced muscle atrophy.Materials and Methods: Three sets of C2C12 cells, including blank control, TNF-α (10 or 20 ng/ml) treatment and a TNF-α (10 or 20 ng/ml) plus psoralen (80 μM) administration were investigated. Cell viability was assessed using Cell Counting Kit-8 (CCK-8) assay. Western blot analysis was used to detect protein expression of atrophic markers. Flowcytometry was used to observe the effect of psoralen on apoptosis. A quantitative real-time PCR (qRT-PCR) assay was performed to detect the mRNA level of miR-675-5P.Results: TNF-α (1, 10, 20 and 100 ng/ml) treatment inhibited C2C12 myoblast viability (P<0.001), while 24 hr of psoralen administration increased the viability, and lowered TNF-α cytotoxicity (P<0.001). MURF1, MAFbx, TRIM62 and GDF15 expressions were significantly increased in TNF-α (10 ng/ml or 20 ng/ml)-treated group (P<0.001), and psoralen could significantly decrease the expression of these proteins (P<0.001). Apoptotic rate of C2C12 myoblasts was increased after TNF-α (10 ng/ml and 20 ng/ml) treatment, and was significantly decreased after psoralen treatment (P<0.001). miR-675-5P was increased in TNF-α-treated C2C12 myoblasts compared to control group, and it was significantly decreased after psoralen treatment.Conclusion: Psoralen could reduce TNF-α-induced cytotoxicity, atrophy and apoptosis in C2C12 myoblasts. The therapeutic effect of psoralen may be achieved by down-regulating miR-675-5P.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
251
256
https://ijbms.mums.ac.ir/article_14290_23547c70f0455d2e3f0811bf0e8c7228.pdf
dx.doi.org/10.22038/ijbms.2019.37469.8939
Synergistic bactericidal activity of a naturally isolated phage and ampicillin against urinary tract infecting Escherichia coli O157
Zahra
Moradpour
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran
author
Nesa
Yousefi
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran
author
Dorna
Sadeghi
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran
author
Abdollah
Ghasemian
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran
author
text
article
2020
eng
Objective(s): Bacteriophages are infectious replicating entities that are under consideration as antimicrobial bioagents to control bacterial infections. As an alternative or supplement to antibiotics, bacteriophages can be used to circumvent the resistance to existing antibiotics. The aim of this study was to assess the synergistic effect of a naturally isolated phage and ampicillin against Escherichia coli O157.Materials and Methods: In the present study, a natural phage against E. coli O157 was isolated, the morphology and molecular characteristics of the phage were identified, and the combination of bacteriophage and antibiotic to combat clinically isolated drug-resistant E. coli O157 was evaluated. Results: The results showed the synergistic action between a naturally isolated phage and ampicillin in solid (disk diffusion test) and liquid culture media. Addition of the isolated phage, gT0E.co-MGY2, to the microbial lawn of bacteria in modified antibiotic disk diffusion test, altered susceptibility pattern of E. coli O157 from resistant to sensitive based on the inhibition zones. Combinations of bacteriophage and ampicillin significantly enhanced the killing of bacterial strains when compared to treatment with ampicillin or phage alone in liquid culture. Moreover, it lasted few hours for ampicillin to reverse the growth of E. coli O157, while the bacteriophage and combination treatment stopped the proliferation of bacteria from the beginning, and this can compensate the delayed onset of antibiotic action.Conclusion: The synergistic action of bacteriophages and antibiotics is an alternative that cannot only be effective against bacterial infections but also contribute to the reduction of antibiotic resistance.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
257
263
https://ijbms.mums.ac.ir/article_14338_0c549e5cd91d1f8f0281fa19d538f455.pdf
dx.doi.org/10.22038/ijbms.2019.37561.8989
Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines
Samila
Farokhimanesh
Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Mehdi
Forouzandeh Moghadam
Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Marzieh
Ebrahimi
Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for StemCell Biology and Technology, ACECR, Tehran, Iran
author
text
article
2020
eng
Objective(s): The growing trend of research demonstrates that dynamic expression of two metastasis repressor classes (metastasis suppressor genes and anti-metastatic miRNA) has a close relationship with tumor invasion and metastasis. Using different strategies, it was revealed that cellular levels of miR-31 and Breast cancer Metastasis Suppressor1 (BRMS1) protein, which are among the most significant modulators of metastasis, have a correlation with the cell’s capability for invading and metastasizing; cells containing higher levels of miR-31 or BRMS1 were less metastatic. This project was carried out to determine whether the combinations of miR-31 and BRMS1 genes are able to enhance the capability of repressing the claudin-low breast cancer cell (MDA-MB-231) invasion. Materials and Methods: This study used a restoration-based approach by miR-31 mimic and optimized BRMS1 gene sequences, which were cloned into a chimeric construct and transfected to the MDA-M231cells. Results: Our data revealed that the simultaneous expression of anti-metastasis miR and metastasis suppressor might inhibit migration and invasion in MDA-MB-231 cells efficiently.Conclusion: This combinatorial use of anti-metastatic miR and gene suggests a new therapeutic intervention for metastasis inhibition in MDA-MB-231.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
264
270
https://ijbms.mums.ac.ir/article_14288_f3e7a762440d6352f0c5afff584f1725.pdf
dx.doi.org/10.22038/ijbms.2019.35674.8500
Renoprotective potential of exogen erythropoietin on experimental ruptured abdominal aortic aneurysm model: An animal study
Gokalp
Altun
Department of Cardiovascular Surgery, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey
author
Yavuz
Cakiroglu
Department of Cardiovascular Surgery, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey
author
Zerrin
Pulathan
Department of Cardiovascular Surgery, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey
author
Esin
Yulug
Department of Histology and Embryology, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey
author
Ahmet
Mentese
Department of Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey
author
text
article
2020
eng
Objective(s): The aim of this study is to investigate the renoprotective effect of erythropoietin (EPO) on hypovolemic shock and ischemia/reperfusion (IR) injury on kidneys as end-organs in an experimentally-created ruptured abdominal aortic aneurysm (rAAA) model.Materials and Methods: Thirty anesthetized Sprague-Dawley male rats were randomized to sham ((Sh n:6) (Sh+EPO n:6)) or shock and I/R groups ((S/IR n:9) (S/IR+EPO n:9)). Additional surgical procedure except aortic exploration was not performed on Sh and Sh+EPO groups. 60 min of shock, 60 min of ischemia, and 120 min of reperfusion were applied on S/IR and S/IR+EPO groups. In the S/IR and S/IR+EPO groups, hemorrhagic shock, lower torso ischemia, and reperfusion were created. At the end of the shock period, saline solutions were separately and equally administered to Sh and S/IR groups, whereas 2000 U/kg EPO was intraperitoneally administered to Sh+EPO and S/IR+EPO groups. At the end of the experimental study, some biochemical and histological parameters were studied in serum and kidney tissues.Results: Biochemical parameters were all significantly increased in the S/IR group compared with the Sh group. These parameters were not statistically significantly different between S/IR+EPO and Sh+EPO groups. In histopathologic examination, EPO prevented high-grade injury.Conclusion: Our data indicate that EPO may have a renoprotective effect and reduce the systemic inflammatory response that resulted from shock and I/R in an experimental model of rAAA.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
2
no.
2020
271
276
https://ijbms.mums.ac.ir/article_14382_6cbb7229f143d48f20ba62bd9ac15625.pdf
dx.doi.org/10.22038/ijbms.2019.36215.8626