The therapeutic approaches of renal recovery after relief of the unilateral ureteral obstruction: A comprehensive review
Ayat
Kaeidi
Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Maryam
Maleki
Department of Physiology, Ilam University of Medical Sciences, Ilam, Iran
author
Ali
Shamsizadeh
Department of Physiology and Pharmacology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Iman
Fatemi
Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran
author
Elham
Hakimizadeh
Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Jalal
Hassanshahi
Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
text
article
2020
eng
Unilateral ureteral obstruction (UUO) as a clinical disorder can cause renal damage. The permanent injury occurs if the obstruction is not relieved. Renal injury can be reversed with UUO removal (RUUO). RUUO attenuates the renal hemodynamic and functional impairment and decreases the renal fibrosis and apoptosis. Nevertheless, kidney injury may continue after RUUO, and synchronous medication therapy seems necessary. However, UUO and post-RUUO periods are also important in final renal recovery. To date, various therapeutic strategies have been applied to develop renal recoverability after RUUO. In animal studies, the effect of some pharmacological agents such as mesenchymal stem cells, anti-inflammation drugs, L-arginine, bone morphogenetic protein-7, epidermal growth factor, allopurinol, renin-angiotensin system antagonists, and endothelin A/B receptor blocker were surveyed in RUUO model. Also, post-RUUO renal recoverability has been studied in human researches. In these studies, the effective strategies have focused on surgery for RUUO creation via urethrotomy, urethroplasty, stent balloon dilatation, and stenting. Accordingly, in this review, we focused on the therapeutic procedure of renal recovery after the RUUO situation in human and animal studies.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1367
1373
https://ijbms.mums.ac.ir/article_16431_74872c540d14df044f3473f2ee771bf8.pdf
dx.doi.org/10.22038/ijbms.2020.41984.9926
Effect of Boswellia species on the metabolic syndrome: A review
Davoud
Mahdian
Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
author
Kazem
Abbaszadeh-Goudarzi
Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
author
Amir
Raoofi
Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
author
Ghazaleh
Dadashizadeh
Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
author
Mina
Abroudi
Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
author
Elahe
Zarepour
Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
author
Hossein
Hosseinzadeh
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2020
eng
The metabolic syndrome, a cluster of metabolic disorders, includes abdominal obesity, hypertension, dyslipidemia, and hyperglycemia leading to insulin resistance, development of diabetes mellitus, and cardiovascular diseases. For the treatment of metabolic syndrome, traditional herbal medicines such as frankincense or Boswellia species have been used due to their anti-inflammatory, anti-oxidant, anti-obesity, antidiabetic, antihypertensive, and hypolipidemic properties. Based on the literature, published evidence up to 2020 about the therapeutic effects of Boswellia species on the metabolic disorder among Medline, Scopus, and Google Scholar were precisely evaluated by keywords such as obesity, diabetes, hyperglycemia, hypertension, blood pressure, dyslipidemia, metabolic syndrome, frankincense, and Boswellia. According to the results, Boswellia species have beneficial effects to control metabolic syndrome and its related disorders such as hyperglycemia, dyslipidemia, hypertension, obesity, diabetes, and its complications. Boswellia species by reducing the resistance to insulin and restoring pancreatic beta cells decrease blood glucose. Also, Boswellia species has antithrombotic and anticoagulant properties that regulate blood pressure. The anti-oxidant properties of Boswellia species modulate the blood lipid profile via reducing TNF-α, IL-1β levels, and increasing the adiponectin level. The therapeutic and protective effects of Boswellia species on metabolic disorders were remarkably confirmed regarding decreasing hyperglycemia, hyperlipidemia, hypertension, and obesity.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1374
1381
https://ijbms.mums.ac.ir/article_16582_5e1fcd26c484c24ffc8c1d89ac6b81ba.pdf
dx.doi.org/10.22038/ijbms.2020.42115.9957
The investigation of the effect of fraxin on hepatotoxicity induced by cisplatin in rats
Fazile Nur
Ekinci Akdemir
Department of Nutrition and Dietetics, High School of Health, Ağrı İbrahim Çeçen University, Ağrı, Turkey
author
Cigdem
Bingol
Department of First and Emergency Aid, Health Services Vocational High School, Ağrı İbrahim Çeçen University, Ağrı, Turkey
author
Serkan
Yildirim
Department of Pathology, Faculty of Veterinary, Atatürk University, Erzurum, Turkey
author
Fatih
Kandemir
Department of Biochemistry, Faculty of Veterinary, Atatürk University, Erzurum, Turkey
author
Sefa
Kucukler
Department of Biochemistry, Faculty of Veterinary, Atatürk University, Erzurum, Turkey
author
Yavuz
Saglam
Department of Pathology, Faculty of Veterinary, Atatürk University, Erzurum, Turkey
author
text
article
2020
eng
Objective(s): This study was designed to assess the effect of fraxin which has various biological properties against liver injury induced by cisplatin.Materials and Methods: In our study, 24 Wistar albino rats were randomly assigned to control, fraxin, cisplatin, and fraxin+cisplatin groups. Cisplatin 12 mg/kg IP and fraxin 40 mg/kg orally were applied. When the experiment ended, the rats were sacrificed and the liver tissues were taken rapidly. Liver tissue specimens were maintained under appropriate conditions. Later, biochemical, histopathological, and immunohistochemical evaluations were performed.Results: According to our biochemical findings, oxidant parameters increased while antioxidant parameters decreased in cisplatin group compared with control group. Antioxidant parameters increased but oxidant parameters decreased in fraxin + cisplatin group compared with the cisplatin group. Immunohistochemical evaluations showed that the expressions of TNF-α and Caspase-3 were negative in control and fraxin groups, whereas severe levels were found in the cisplatin group. However, it was determined that the expressions of TNF-α and Caspase-3 were in mild levels in fraxin + cisplatin treatment group. In addition, it was observed that the increase of pathological markers such as coagulation necrosis, hydropic degeneration, dilatation in sinusoid, and hyperemia in the cisplatin group were compatible with our biochemical and immunohistochemical findings. Conclusion: Biochemical, immunohistochemical, and histopathological results revealed that fraxin was effective in relieving cisplatin-induced liver damage.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1382
1387
https://ijbms.mums.ac.ir/article_16488_0e18d4496d2e37e380037703a37a44a8.pdf
dx.doi.org/10.22038/ijbms.2020.38773.9200
Synthesis and biological evaluation of oxazinonaphthalene-3-one derivatives as potential anticancer agents and tubulin inhibitors
Salimeh
Mirzaei
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
Maqsudjon
Qayumov
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
Fahimeh
Ganji
Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Javad
Behravan
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
Razieh
Ghodsi
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2020
eng
Objective(s): In the present study, a new series of oxazinonaphthalene-3-one analogs was designed and synthesized as novel tubulin inhibitors.Materials and Methods: The cytotoxic activity of the synthesized compounds was evaluated against four human cancer cell lines including A2780 (human ovarian carcinoma), A2780/RCIS (cisplatin resistant human ovarian carcinoma), MCF-7 (human breast cancer cells), and MCF-7/MX (mitoxantrone resistant human breast cancer cells), those compounds which demonstrated the most antiproliferative activity in the MTT test were selected to investigate their tubulin inhibition activity and their effects on cell cycle arrest (at the G2/M phase). Moreover, molecular docking studies of the selected compounds in the catalytic site of tubulin (PDB ID: 4O2B) were carried out to describe the results of biological experiments.Results: Most of our compounds exhibited significant to moderate cytotoxic activity against four human cancer cell lines. Among them, Compounds 4d, 5c, and 5g, possessing trimethoxy phenyl, showed the most antiproliferative activity with IC50 values ranging from 4.47 to 52.8 μM.Conclusion: The flow cytometric analysis of A2780 cancer cell line treated with compounds 4d, 5c, and 5g showed that these compounds induced cell cycle arrest at the G2/M phase. Compound 5g, the most antiproliferative compound, inhibited tubulin polymerization in a dose-dependent manner. Molecular docking studies of 5g into the colchicine-binding site of tubulin displayed a possible mode of interaction between this compound and tubulin.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1388
1395
https://ijbms.mums.ac.ir/article_16623_b36d3247ce30ba1a81e66b754a831a1d.pdf
dx.doi.org/10.22038/ijbms.2020.40845.9648
Survey of various carbapenem-resistant mechanisms of Acinetobacter baumannii and Pseudomonas aeruginosa isolated from clinical samples in Iran
Leila
Azimi
Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
Fatemeh
Fallah
Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
Abdollah
Karimi
Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
Mehdi
Shirdoust
Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
Taher
Azimi
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
author
Iraj Sedighi
Sedighi
Pediatric department, faculty of medicine, Hamadan University of Medical Sciences, Hamadan, Iran
author
Mohammad
Rahbar
Department of Microbiology, Reference Health Laboratories Research Center, Ministry of Health and Medical Education, Tehran, Iran
author
Shahnaz
Armin
Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
text
article
2020
eng
Objective(s): Pseudomonas aeruginosa and Acinetobacter baumannii resist antibiotics by different intrinsic and acquired mechanisms. This study aims to define various carbapenem-resistant mechanisms of isolated P. aeruginosa and A. baumannii from nine different provinces of Iran.Materials and Methods: In this cross-sectional study, all carbapenem-resistant P. aeruginosa and A. baumannii samples from nine provinces of Iran on a one-year time horizon were gathered. Modified Hedge Test (MHT) and Carba NP-Test were applied to the identification of producing-carbapenemase strains. The most important carbapenemase genes recognized by PCR and gene overexpression of the efflux pump were surveyed by efflux pump inhibitors (EPIs) and confirmed by Real-Time PCR. Results: Twenty-one percent and 43.5% of P. aeruginosa and A. baumannii isolates were resistant to carbapenem, respectively. MHT and Carba-NP tests identified 21% and 11% carbapenemase-producing strains in these Gram-negative bacteria, respectively. NDM-1 was the most prevalently detected carbapenemase in P. aeruginosa; OXA-51 and OXA-23 were the most significant genes in A. baumannii. EPIs identified active efflux pumps in 20% and 28% of P. aeruginosa and A. baumannii, respectively. Real-time PCR confirmed gene overexpression of efflux pumps in 54% and 30% of positive EPIs in P. aeruginosa and A. baumannii, respectively. Conclusion: P. aeruginosa and A. baumannii may become multi-drug-resistant (MDR) and Extensively Drug-Resistant (XDR) strains and cause a high rate of mortality and morbidity. Thus, it is of necessity to prohibit the spread of antibiotic-resistant strains in hospitals.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1396
1400
https://ijbms.mums.ac.ir/article_16691_4b991b489eb1b6d5c2c5d23af19699db.pdf
dx.doi.org/10.22038/ijbms.2020.44853.10463
Gallic acid from Terminalia chebula inhibited the growth of esophageal carcinoma cells by suppressing the Hippo signal pathway
Gui-Li
Sun
Department of Oncology, Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao 028000, China
author
Dong
Wang
Department Oncology of Mongolian-Western Medicine, Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao 028007, China
author
text
article
2020
eng
Objective(s): To explore the molecular mechanism of gallic acid (GA) from Terminalia chebula in suppressing the growth of esophageal carcinoma (EC).Materials and Methods: Human EC cells (EC9706 and KYSE450) were treated with different concentrations of GA (10, 20, and 40 μg/ml), which were subjected to 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay, plate clone formation assay, annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining, and Western blotting. EC mice were divided into model, 0.3% GA, and 1% GA groups to observe the tumor volume and the expressions of YAP, TAZ, Ki-67, and caspase-3 in tumor tissues. Results: GA decreased cell viability and colony formation of EC9706 and KYSE450 cells, which was more obvious as the concentration increased. In addition, GA promoted cell apoptosis in a concentration-dependent manner with the up-regulation of pro-apoptotic proteins (Bax, cleaved caspase-3, and cleaved caspase-9) and nuclear YAP/TAZ, as well as the down-regulation of anti-apoptotic protein Bcl-2 and the levels of p-YAP and p-TAZ. Moreover, GA decreased the growth of xenograft tumor in vivo, with the reduction in the tumor volume and the reduction of YAP and TAZ expressions in the tumor tissues. In addition, Ki-67 expression in GA groups was lower than those in the Model group, with the increase in caspase-3 expression in the tumor tissues. Changes aforementioned were obviously shown in the 0.3% GA group. Conclusion: GA blocked the activity of the Hippo pathway to suppress cell proliferation of EC and facilitate cell apoptosis, which is expected to be a novel strategy for treatment of EC.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1401
1408
https://ijbms.mums.ac.ir/article_16433_affdc8360035c97c1e714c4fd392f725.pdf
dx.doi.org/10.22038/ijbms.2020.42283.9982
In silico analysis and expression of a new chimeric antigen as a vaccine candidate against cutaneous leishmaniasis
Leila
Motamedpour
Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
author
Abdolhossein
Dalimi
Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
author
Majid
Pirestani
Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
author
Fatemeh
Ghafarifar
Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
author
text
article
2020
eng
Objective(s): Since leishmaniasis is one of the health problems in many countries, the development of preventive vaccines against it is a top priority. Peptide vaccines may be a new way to fight the Leishmania infection. In this study, a silicon method was used to predict and analyze B and T cells to produce a vaccine against cutaneous leishmaniasis.Materials and Methods: Immunodominant epitope of Leishmania were selected from four TSA, LPG3, GP63, and Lmsti1 antigens and linked together using a flexible linker (SAPGTP). The antigenic and allergenic features, 2D and 3D structures, and physicochemical features of a chimeric protein were predicted. Finally, through bioinformatics methods, the mRNA structure was predicted and was produced chemically and cloned into the pLEXY-neo2 vector.Results: Results indicated, polytope had no allergenic properties, but its antigenicity was estimated to be 0.92%. The amino acids numbers, molecular weight as well as negative and positive charge residuals were estimated 390, ~41KDa, 41, and 30, respectively. The results showed that the designed polytope has 50 post-translationally modified sites. Also, the secondary structure of the protein is composed of 25.38% alpha-helix, 12.31% extended strand, and 62.31% random coil. The results of SDS-PAGE and Western blotting revealed the recombinant protein with ̴ 41 kDa. The results of Ramachandran plot showed that 96%, 2.7%, and 1.3% of amino acid residues were located in the preferred, permitted, and outlier areas, respectively. Conclusion: It is expected that the TLGL polytope will produce a cellular immune response. Therefore, the polytope could be a good candidate for an anti-leishmanial vaccine.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1409
1418
https://ijbms.mums.ac.ir/article_16432_5687b6c91296e5573129d29e057b79b1.pdf
dx.doi.org/10.22038/ijbms.2020.45394.10561
MicroRNA-155 from sputum as noninvasive biomarker for diagnosis of active pulmonary tuberculosis
Ying
Hua
Vascular Disease Research Center and Basic Medical Laboratory, the Second affiliated hospital of Wannan Medical College, Kangfu Road 10#, Wuhu 241000, Anhui province, PR China
author
FengYing
Sun
Department of clinical laboratory, the Second peoples’ hospital of Wuhu city, Jiuhua Zhong Road 259#, Wuhu 241000, Anhui province, PR China
author
YanHong
Wu
Department of microbiology, Wannan Medical College, Wenchang Xi Road 22#, Wuhu 241000, Anhui province, PR China
author
YouMing
Huang
Vascular Disease Research Center and Basic Medical Laboratory, the Second affiliated hospital of Wannan Medical College, Kangfu Road 10#, Wuhu 241000, Anhui province, PR China
author
FaYou
Zhou
Vascular Disease Research Center and Basic Medical Laboratory, the Second affiliated hospital of Wannan Medical College, Kangfu Road 10#, Wuhu 241000, Anhui province, PR China
author
HongXiang
Zhang
Vascular Disease Research Center and Basic Medical Laboratory, the Second affiliated hospital of Wannan Medical College, Kangfu Road 10#, Wuhu 241000, Anhui province, PR China
author
XiaoLei
Tang
Vascular Disease Research Center and Basic Medical Laboratory, the Second affiliated hospital of Wannan Medical College, Kangfu Road 10#, Wuhu 241000, Anhui province, PR China
author
text
article
2020
eng
Objective(s): Tuberculosis (TB), caused by Mycobacterium tuberculosis, is a widespread infectious disease around the world. Early diagnosis is always important in order to avoid spreading. At present, many studies have confirmed that microRNA (miRNA) could be a useful tool for diagnosis. This study aimed to evaluate whether miRNAs could be regarded as a noninvasive diagnosis biomarker from sputum for pulmonary tuberculosis (PTB).Materials and Methods: The M. tuberculosis strain H37Rv was incubated and cultured with human macrophage line THP-1. The total RNA was extracted from the THP-1 cells for detection. Six increased expressions of miRNAs were selected by miRNA microarray chips and the miRNAs were confirmed by qRT-PCR in the M. tuberculosis infection cell model. At last, the efficiency of other methods was compared with using miRNA.Results: Only miR-155 showed a better diagnostic value for PTB than the other five miRNAs to distinguish PTB from non-PTB, including pneumonia, lung cancer, and unexplained pulmonary nodules. Next, we detected and analyzed the results of 68 PTB patients and 122 non-PTB, the sensitivity and specificity of miR-155 detection was 94.1% and 87.7%, respectively. It was higher than sputum smear detection and anti-TB antibody detection. But slightly lower than ELISpot (97%, P=0.404). Interestingly, the ranking of sputum smear by Ziehl-Neelsen staining had positive correlation with the expression level of miR-155 in smear-positive sputum (R2=0.8443, p <0.05).Conclusion: Our research suggested that miR-155 may be an efficiency biomarker for active PTB diagnosis and bacteria-loads evaluation.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1419
1425
https://ijbms.mums.ac.ir/article_16489_5ebcf697e3ec87bff3c6e10c2661d304.pdf
dx.doi.org/10.22038/ijbms.2020.44029.10324
Role of organic and ceramic biomaterials on bone healing and regeneration: An experimental study with significant value in translational tissue engineering and regenerative medicine
Ali
Moshiri
Razi Drug Research Center, and department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
author
Neda
Tekyieh Maroof
Razi Drug Research Center, and department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
author
Ali Mohammad
Sharifi
Razi Drug Research Center, and department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
author
text
article
2020
eng
Objective(s): We investigated the role of various biomaterials on cell viability and in healing of an experimentally induced femoral bone hole model in rats.Materials and Methods: Cell viability and cytotoxicity of gelatin (Gel; 50 µg/µl), chitosan (Chi; 20 µg/µl), hydroxyapatite (HA; 50 µg/µl), nanohydroxyapatite (nHA; 10 µg/µl), three-calcium phosphate (TCP; 50 µg/µl) and strontium carbonate (Sr; 10 µg/µl) were evaluated on hADSCs via MTT assay. In vivo femoral drill-bone hole model was produced in rats that were either left untreated or treated with autograft, Gel, Chi, HA, nHA, TCP and Sr, respectively. The animals were euthanized after 30 days. Their bone holes were evaluated by gross-pathology, histopathology, SEM and radiography. Also, their dry matter, bone ash and mineral density were measured.Results: Both the Gel and Chi showed cytotoxicity, while nHA had no role on cytotoxicity and cell-viability. All the HA, TCP and Sr significantly improved cell viability when compared to controls (p <0.05). Both the Gel and Chi had no role on osteoconduction and osteoinduction. Compared to HA, nHA showed superior role in increasing new bone formation, mineral density and ash (p <0.05). In contrast to HA and nHA, both the TCP and Sr showed superior morphological, radiographical and biochemical properties on bone healing (p <0.05). TCP and Sr showed the most effective osteoconduction and osteoinduction, respectively. In the Sr group, the most mature type of osteons formed.Conclusion: Various biomaterials have different in vivo efficacy during bone regeneration. TCP was found to be the best material for osteoconduction and Sr for osteoinduction.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1426
1438
https://ijbms.mums.ac.ir/article_16576_794c3acb5848c77fdc09af92da6c5f7b.pdf
dx.doi.org/10.22038/ijbms.2020.46228.10707
In vivo therapeutic effects of colorectal cancer cell-derived exosomes
Ali
Ganji
Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran
author
Iman
Farahani
Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran
author
Mana
Shojapuor
Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran
author
Ali
Ghazavi
Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran
author
Ghasem
Mosayebi
Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran
author
text
article
2020
eng
Objective(s): Exosomes are nano-sized structures with lipid bilayer membranes that can be secreted by cancer cells. They play an important role in the biology of the tumor extracellular matrix. Exosomes may contain and transfer tumor antigens to dendritic cells to trigger T cell-mediated anti-tumor immune responses. Materials and Methods: BALB/c mice bearing CT26 colorectal cancer were treated subcutaneously with purified exosomes from analogous tumor cells. The mice were analyzed with respect to tumor size, survival, and anti-tumor immunity responses, including gene expression of cytokines and flowcytometry analysis of T lymphocytes. Results: The rate of tumor size growth in the exosome-treated group significantly decreased (p <0.05), and the flow cytometry results showed a significant reduction in the spleen regulatory T cells (Tregs) count of the exosome-treated group, compared with the untreated group (P=0.02). Although the increase in the serum level of interferon-γ (IFN-γ) and the number of cytotoxic CD8 T lymphocytes (CTLs) in the spleen tissue was not significant (P>0.05), the gene expression of IFN-γ increased significantly (P=0.006). Conclusion: The present results revealed that subcutaneous administration of tumor-derived exosomes could effectively lead to the inhibition of tumor progression by decreasing the number of Treg cells and up-regulation of the IFN-γ gene. Therefore, tumor-derived exosomes can be used as potential vaccines in cancer immunotherapy.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1439
1444
https://ijbms.mums.ac.ir/article_16577_cd27ae9c843c8f0dd2c5e8bab26b00af.pdf
dx.doi.org/10.22038/ijbms.2020.46465.10730
Corallodiscus flabellata B. L. Burtt extract alleviates lipopolysaccharide/D-galactosamine-induced acute liver failure and brain injury by inhibiting oxidative stress, apoptosis, and inflammation
Benke
Li
College of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China
author
Mengnan
Zeng
College of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China
author
Beibei
Zhang
College of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China
author
Yuxuan
Kan
College of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China
author
Shengchao
Wang
College of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China
author
Yangyang
Wang
College of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China
author
Yuanyuan
Wu
College of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China
author
Ruiqi
Xu
College of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China
author
Weisheng
Feng
College of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China
author
Xiaoke
Zheng
College of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China
author
text
article
2020
eng
Objective(s): Corallodiscus flabellata B. L. Burtt (CF) is distributed along liver meridian, with a possible beneficial effect in the progression of acute liver failure. Therefore, the present study investigates the effect of CF extract on rats with acute liver failure.Materials and Methods: Rats were divided into four experimental groups: Control, Lipopolysaccharide (LPS)/D-Galactosamine (D-GalN) (L/D), Wu Ling Powder + L/D (WLP+L/D) and CF + L/D. Animals were gavage for 7 days, after which all animals except the control group were injected intraperitoneally with LPS and D-GalN to induce acute liver failure. Subsequently, the urine was collected for the next 8 hr, and the liver pathological changes were observed. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), inflammatory factor and oxidative stress-related indicators were measured. The levels of reactive oxygen species (ROS), apoptosis marker in the liver, water content and aquaporin (AQPs) in the brain were detected. The concentration of ions and osmolality of urine and serum were determined.Results: The results show that CF significantly improved the damage of liver and brain tissue, and reversed the changes of serum ALT, AST, inflammatory factor and Cl-. It modulated oxidative stress-related indicators, reduced the content of ROS, apoptosis markers, water content, the level of Cl- ions and osmolality in the urine and the expression of AQP1, and AQP4 in the brain, and increased the urine output. Conclusion: It was found that the CF extract could alleviate the L/D induced acute liver failure by regulating the hepatocyte apoptosis and AQPs expression in the brain.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1445
1452
https://ijbms.mums.ac.ir/article_16487_2b0dc3e7f8ca0183c2d5f878f70768da.pdf
dx.doi.org/10.22038/ijbms.2020.45437.10567
Human amniotic membrane mesenchymal stem cells-conditioned medium attenuates myocardial ischemia-reperfusion injury in rats by targeting oxidative stress
Behnaz
Mokhtari
Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran
author
Yaser
Azizi
Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran
author
Aliakbar
Rostami Abookheili
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran
author
Nahid
Aboutaleb
Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran
author
Donya
Nazarinia
Department of Physiology, School of Paramedical Sciences, Dezful University of Medical Sciences, Dezful, Iran
author
Nasim
Naderi
Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
author
text
article
2020
eng
Objective(s): Ischemic heart diseases (IHD) are one of the major causes of death worldwide. Studies have shown that mesenchymal stem cells can secrete and release conditioned medium (CM) which has biological activities and can repair tissue injury. This study aimed to investigate the effects of human amniotic membrane mesenchymal stem cells (hAMCs)-CM on myocardial ischemia/reperfusion (I/R) injury in rats by targeting oxidative stress. Materials and Methods: Male Wistar rats (40 rats, weighing 200–250 g) were randomly divided into four groups: Sham, myocardial infarction (MI), MI + culture media, and MI + conditioned medium. MI was induced by ligation of the left anterior descending coronary artery for 30 min. After 15 min of reperfusion, intramyocardial injections of hAMCs-CM or culture media (150 μl) were performed. At the end of the experiment, serum levels of cardiac troponin-I (cTn-I), myocardial levels of malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx), as well as cardiac histological changes were evaluated.Results: HAMCs-CM significantly decreased cTn-I and MDA levels and increased SOD and GPx activities (p <0.05). In addition, hAMCs-CM improved cardiac histological changes and decreased myocardial injury percentage (p <0.05). Conclusion: This study showed that hAMCs-CM has cardioprotective effects in the I/R injury condition. Reduction of oxidative stress by hAMCs-CM plays a significant role in this context. Based on the results of this study, it can be concluded that hAMCs-CM can be offered as a therapeutic candidate for I/R injury in the future, but more research is needed.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1453
1461
https://ijbms.mums.ac.ir/article_16581_e218d7b6a8045d9079ea3762b8c2e57e.pdf
dx.doi.org/10.22038/ijbms.2020.47572.10952
Therapeutic activities of naringenin on efavirenz-induced sleep-like disorder in the midbrain of white albino mice
Olufunke
Dosumu
Department of Anatomy, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria
author
Edidiong
Akang
Department of Anatomy, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria
author
Oluwatomisin
Faniyan
Department of Anatomy, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria
author
Alani
Akanmu
Department of Haematology and Blood Transfusion, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria
author
text
article
2020
eng
Objective(s): Efavirenz, has proven to be effective in suppressing human immunodeficiency virus (HIV) viral load; however, complaints of sleep disorders including hallucination, and insomnia have greatly contributed to non-adherence to antiretroviral therapy. This study aimed at investigating therapeutic activities of naringenin on efavirenz-induced sleep disorder.Materials and Methods: Sixty mice were divided into six groups of control, combination antiretroviral therapy (cART), efavirenz, naringenin, naringenin/efavirenz and naringenin/cART. Efavirenz, cART, and naringenin were administered orally and daily at 15 mg/kg, 24 mg/kg and 50 mg/kg, respectively for 28 days. Post neurobehavioral test, oxidative stress, histology and immunohistochemistry for dopamine were carried out after administration process.Results: Efavirenz (p <0.0001) and cART (p <0.01) significantly increased immobility during open field (p <0.01), escape time in seconds (sec) in Morris water maze (p <0.001) and numbers of head-twitch response (HTR) (p <0.0001). Similarly, there was a significant increase in malondialdehyde (MDA) (p <0.0001) and decreased superoxide dismutase (SOD) (p <0.001) and reduced glutathione (GSH) (p <0.001); however, naringenin-treated groups potentiated anti-oxidant function by reducing oxidative stress (p <0.01). Histological evaluation demonstrated severe neurodegeneration, vacuolization and pyknosis in efavirenz and cART compared to naringenin groups. Dopaminergic neurons using immunohistochemial antibody (tyrosine hydroxylase) staining showed poor immunoreactivity in efavirenz and cART in contrast to naringenin groups.Conclusion: Efavirenz and cART have the potential of inducing sleep disorder possibly due to their capability to trigger inflammation and deplete dopamine level. However, naringenin has proven to be effective in ameliorating these damages.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1462
1470
https://ijbms.mums.ac.ir/article_16641_4e6a04151dcc0f83667ad62a37c20865.pdf
dx.doi.org/10.22038/ijbms.2020.47043.10852
Evaluation of antibacterial activity of enterocin A-colicin E1 fusion peptide
Hadis
Fathizadeh
Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
author
Mahmood
Saffari
Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
author
Davoud
Esmaeili
Department of Microbiology and Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
author
Rezvan
Moniri
Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
author
Morteza
Salimian
Anatomical Science Research Center, Kashan University of Medical Sciences, Kashan, Iran
author
text
article
2020
eng
Objective(s): Bacterial resistance to most common antibiotics is a harbinger of the requirement to find novel anti-infective, antimicrobials agents, and increase innovative strategies to struggle them. Numerous bacteria produce small peptides with antimicrobial activities called bacteriocin. This study aimed to investigate the antibacterial properties of the fusion protein of Enterocin A and Colicin E1 modified against pathogens.Materials and Methods: Analysis of recombinant bacteriocin Enterocin A and Colicin E1 (ent A-col E1) was performed to assay the stability and antibacterial activity of this fusion protein. The pET-22b vector was employed to express the coding sequence of the ent A-col E1 peptide in Escherichia coli BL21 (DE3). Minimum inhibitory concentration (MIC), disk diffusion, and time-kill tests were performed to evaluate the antibacterial activity of the ent A-col E1 against Pseudomonas aeruginosa (ATCC 9027), Escherichia coli (ATCC 10536), Enterococcus faecalis (ATCC 29212), and Staphylococcus aureus (ATCC 33591).Results: The suggested recombinant peptide had good antibacterial activity against both Gram-negative and Gram-positive pathogens. It has also good stability at various temperatures, pH levels, and salt concentrations.Conclusion: Because bacteriocins are harmless compounds, they can be recommended as therapeutic or preventive supplements to control pathogens. According to the obtained results, the ent A-col E1 peptide can serve as an efficient antibacterial compound to treat or prevent bacterial infections.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1471
1479
https://ijbms.mums.ac.ir/article_16640_958d331f9ce8afa2922454971acecc8c.pdf
dx.doi.org/10.22038/ijbms.2020.47826.11004
Effects of berberine hydrochloride on methamphetamine-induced anxiety behaviors and relapse in rats
Leila
Rezaeian
Department of Addiction Studies, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
author
Hamid
Kalalian Moghadam
Addiction Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
author
Fahimeh
Mohseni
Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
author
Mehdi
Khaksari
Department of Physiology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
author
Raheleh
Rafaiee
Department of Neuroscience, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran
author
text
article
2020
eng
Objective(s): This research aimed at evaluating the effect of berberine hydrochloride on anxiety-related behaviors induced by methamphetamine (METH) in rats, assessing relapse and neuroprotective effects. Materials and Methods: 27 male Wistar rats were randomly assigned into groups of Control, METH-withdrawal (METH addiction and subsequent withdrawal), and METH addiction with berberine hydrochloride oral treatment (100 mg/kg/per day) during the three weeks of withdrawal. Two groups received inhaled METH self-administration for two weeks (up to 10 mg/kg). The elevated plus maze (EPM) test and open field test (OFT) were carried out one day after the last berberine treatment and relapse was assessed by conditional place preference (CPP) test. TUNEL assay and immunofluorescence staining for NF-κB, TLR4, Sirt1, and α-actin expression in the hippocampus were tested. Results: After 3 weeks withdrawal, berberine hydrochloride decreased locomotor activity and reduced anxiety-related behaviors in comparison with the METH-withdrawal group (p <0.001). The obtained results from CPP showed that berberine significantly reduced relapse (p <0.01). Significantly decrease in activation of TLR4, Sirt1, and α-actin in METH-withdrawal group was found and the percentage of TLR4, Sirt1, and α-actin improved in berberine-treated group (p <0.001). A significant activity rise of NF-κB of cells in the METH-withdrawal group was detected compared to berberine-treated and control groups (p <0.001). Conclusion: Treatment with berberine hydrochloride via modulating neuroinflammation may be considered as a potential new medication for the treatment of METH addiction and relapse. The histological assays supported the neuroprotective effects of berberine in the hippocampus.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1480
1488
https://ijbms.mums.ac.ir/article_16648_a7d53e7e3d07fa064a417eb3716979b7.pdf
dx.doi.org/10.22038/ijbms.2020.47285.10884
Distribution of Staphylococcal cassette chromosome mecA (SCCmec) types among coagulase-negative Staphylococci isolates from healthcare workers in the North-West of Iran
Zahra
Shokravi
Department of Microbiology, Faculty of Basic Science, Islamic Azad University, Science and Research Branch of Tehran, Markazi, Iran
author
Mehdi
Haseli
Biotechnology Department, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
author
Laleh
Mehrad
Department of Microbiology, Faculty of Basic Science, Islamic Azad University, Science and Research Branch of Tehran, Markazi, Iran
author
Ali
Ramazani
Biotechnology Department, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
author
text
article
2020
eng
Objective(s): Methicillin-resistant coagulase-negative Staphylococci (MR-CoNS) are recognized as one of the major causes of healthcare-associated infections in hospitals. The present investigation aimed to study the prevalence of Staphylococcal cassette chromosome mec (SCCmec) types, along with aminoglycoside modifying enzymes (AMEs) genes in the nasal carriage of MR-CoNS in the north-west of Iran.Materials and Methods: To assess the potential of coagulase-negative Staphylococci as hidden reservoirs for antibiotic resistance, we analyzed the antimicrobial susceptibility of MR-CoNS using the disk diffusion method. In addition, PCR and multiplex PCR assays were performed to determine the prevalence of AME encoding genes and SCCmec types in methicillin-resistant coagulase-negative Staphylococci isolates.Results: A total of 51 MR-CoNS isolates were recovered from the anterior nares of healthcare workers. The observed resistance rates to tobramycin, gentamicin, cotrimoxazole, kanamycin, erythromycin, tetracycline, and ciprofloxacin were 74.5%, 68.5%, 57%, 53%, 51%, 49%, and 8%, respectively. Of the 51 tested MR-CoNS isolates, 2(4%) were harboring SCCmec type I, four (8%) were type II, six (12%) type III, eleven (21.6%) type IVa, two (4%) type IVb, two (4%) type IVc, six (12%) type IVd, and two (4%) type V. The rates of prevalence of the aminoglycoside modifying enzyme genes were as follows: aac (6′)/aph (2′′) (28 cases, 55 %), ant (4′)-Ia (20 cases, 39%), and the aph (3´)-IIIa gene (9 cases, 17.6 %).Conclusion: Subtypes IVa and IVd were the most prevalent SCC elements, and aac (6′)/aph (2′′) was the most common AME gene detected among the MR-CoNS isolates.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1489
1493
https://ijbms.mums.ac.ir/article_16642_8bca89e642c14171b6cc9fa89a004ac8.pdf
dx.doi.org/10.22038/ijbms.2020.48481.11127
An effective weapon against biofilm consortia and small colony variants of MRSA
Zulfiqar
Mirani
Microbiology-FMRRC-Pakistan Council of Scientific and Industrial Research Laboratories Complex Karachi, Pakistan
author
Shaista
Urooj
Bioscience, Shaheed Zulfiqar Ali Bhutto Institute of Science & Technology Karachi, Pakistan
author
Muhammad
Khan
Microbiology-FMRRC-Pakistan Council of Scientific and Industrial Research Laboratories Complex Karachi, Pakistan
author
Abdul
Khan
Microbiology-FMRRC-Pakistan Council of Scientific and Industrial Research Laboratories Complex Karachi, Pakistan
author
Izhar
Shaikh
Pakistan Council of Scientific and Industrial Research-Islamabad, Pakistan
author
Anila
Siddiqui
Microbiology-FMRRC-Pakistan Council of Scientific and Industrial Research Laboratories Complex Karachi, Pakistan
author
text
article
2020
eng
Objective(s): This study was designed to investigate the effect of AgNPs (10 nm and 30 nm) on different phenotypes of Staphylococcus aureus biofilm consortia.Materials and Methods: A total of eighteen biofilm-producing isolates of Methicillin-Resistant S. aureus (MRSA) were used in the present study. Tube methods, Congo-red agar method, and scanning electron microscopy (SEM) were used to study biofilm phenotypes. Population analysis assay on a tryptone agar (TSA) plate was applied to study the different phenotypes of biofilm consortia. The effect of AgNPs was evaluated by broth dilution assay. Results: Results showed that biofilm consortia harbor different phenotypes, i.e., planktonic, metabolically inactive cells, and small colony variants (SCVs) or persister cells. The focus of the present study is the effect of AgNPs on biofilm consortia of MRSA, particularly on the SCVs population. Large size AgNPs (30 nm) were unable to diffuse through extracellular matrix material coverings of the biofilm consortia; they were only active against the planktonic population that occupies the outer surface of consortia. The smaller AgNPs (10 nm), on the other hand, were found to diffuse through the matrix material and hence were effective against planktonic as well as metabolically inactive population of consortia. Moreover, 30 nm AgNPs take 6 hr to disperse off and kill planktonic and upper surface indwellers. The 10 nm AgNPs disperse and kill the majority of biofilm indwellers within 20 min. Conclusion: The present study showed that 10 nm AgNPs can easily penetrate inside the biofilm and are active against all of the indwellers of consortia.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
11
no.
2020
1494
1498
https://ijbms.mums.ac.ir/article_16486_55d6e6b6a3740a022fe18fa0ea384b75.pdf
dx.doi.org/10.22038/ijbms.2020.46384.10712