The frequency of CCR5 promoter polymorphisms and CCR5 32 mutation in Iranian populations
Mohammad
Zare-Bidaki
Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Masoud
Karimi-Googheri
Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
author
Gholamhossein
Hassanshahi
Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Nahid
Zainodini
Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Mohammad
Kazemi Arababadi
Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
text
article
2015
eng
Evidence showed that chemokines serve as pro-migratory factors for immune cells. CCL3, CCL4 and CCL5, as the main CC chemokines subfamily members, activate immune cells through binding to CC chemokine receptor 5 or CCR5. Macrophages, NK cells and T lymphocytes express CCR5 and thus, affected CCR5 expression or functions could be associated with altered immune responses. Deletion of 32 base pairs (D 32) in the exon 1 of the CCR5 gene, which is known as CCR5 D 32 mutation causes down regulation and malfunction of the molecule. Furthermore, it has been evidenced that three polymorphisms in the promoter region of CCR5 modulate its expression. Altered CCR5 expression in microbial infection and immune related diseases have been reported by several researchers but the role of CCR5 promoter polymorphisms and CCR5 D 32 mutation in Iranian patients suffering from these diseases are controversial. Due to the fact that Iranian people have different genetic backgrounds compared to other ethnics, hence, CCR5 promoter polymorphisms and CCR5 D 32 mutation association with the diseases may be different in Iranian patients. Therefore, this review addresses the most recent information regarding the prevalence as well as association of the mutation and polymorphisms in Iranian patients with microbial infection and immune related diseases as along with normal population.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
312
316
https://ijbms.mums.ac.ir/article_4278_29d40ed5f135e3a7d405db47b6caf413.pdf
dx.doi.org/10.22038/ijbms.2015.4278
Biochemical and morphological changes in bone marrow mesenchymal stem cells induced by treatment of rats with p-Nonylphenol
Mohammad Hossein
Abnosi
Biology Department, Faculty of Sciences, Arak University, Arak, Iran
author
Elham
Shojafar
Biology Department, Faculty of Sciences, Arak University, Arak, Iran
author
text
article
2015
eng
Objective(s):In previous investigations, we have shown para-nonylphenol (p-NP) caused significant reduction of proliferation and differentiation of rat bone marrow mesenchymal stem cells (MSCs) in vitro. In this study, we first treat the rats with p-NP, then carried out the biochemical and morphological studies on MSCs. Materials and Methods: Proliferation property of cells was evaluated with the help of MTT assay, trypan blue, population doubling number, and colony forming assay. Differentiation property was evaluated with quantitative alizarin red assay, measurement of alkaline phosphatase (ALP) activity as well as intracellular calcium content. In addition; morphological study, TUNEL test, activated caspase assay, and comet assay were performed to evaluate the mechanism of the cell death. Results: The results showed significant reduction in the colony-forming-ability and population-doubling-number of extracted cells when compared to control ones. In addition, it was revealed that the p-NP treatment of rats caused significant reduction in nuclear diameter, cytoplasm shrinkage, and induction of caspase-dependent-apoptosis. Also there was significant reduction in ALP activity, intracellular calcium content, and intracellular matrix following osteogenic differentiation. Conclusion: As MSCs are the cellular back up for bone remodeling and repair, we suggest more investigations to be conducted regarding the correlation between the increasing number of patients suffering from osteoporosis and p-NP toxicity. Also, we strongly recommend WHO and local health organization to prevent industries of using p-NP in formulation of industrial products which may cause changes in proliferation and differentiation properties of stem cells.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
317
324
https://ijbms.mums.ac.ir/article_4279_e0641f4713d1b11c27f8fdc691180354.pdf
dx.doi.org/10.22038/ijbms.2015.4279
Male germ-like cell differentiation potential of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells in co-culture with human placenta cells in presence of BMP4 and retinoic acid
Nahid
Ataie nejad
Department of Reproductive Biology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
Fardin
Amidi
Department of Anatomical Sciences, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
Marziyeh
Agha Hoseini
IVF Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
author
Karim
Nayer Nia
Institute for Molecular Medicine and Cell Therapy, Düsseldorf, Germany and GENEOCELL, Advanced Molecular & Cellular Technologies, Tehran, Iran
author
Mehryar
Habibi
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
author
Abdol Mohammad
Kajbafzadeh
Pediatric Urology Research Center, Children Hospital Medical Center, Tehran University of Medical Sciences, Tehran, Iran
author
Zohreh
Mazaheri
Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Nazila
Yamini
Department of Reproductive Biology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
text
article
2015
eng
Objective(s):Mesenchymal stem cells (MSCs) derived from Wharton’s jelly (WJ-MSCs) are now much more appealing for cell-based infertility therapy. Hence, WJ-MSCs differentiation toward germ layer cells for cell therapy purposes is currently under intensive study.
Materials and Methods: MSCs were isolated from human Wharton’s jelly and treated with BMP4, retinoic acid (RA) or co-cultured on human amniotic epithelial (HAE) and chorionic plate (HCP) placenta feeder cells. profile of POU5F1, Fragilis, Plzf, DDX4, Piwil2, Stra8, Dazl, β1- and α6-integrins (ITΒ1, ITA6) genes expression as germ cell markers were analyzed using RT-PCR and real-time PCR. Immunocytochemistry of surface markers were conducted.
Results: After 3 weeks treatment with different reagents and co-culture system, morphology of WJ-MSCs changed to shiny clusters and germ cell specific markers in mRNA were up-regulated in both placental feeder + RA and BMP4 + RA. Induction of hWJ-MSCs with BMP4 in presence of RA resulted in significant up-regulation (P≤0.05) of all germ cell specific genes (c-Kit; 2.84±0.59, DDX4; 1.69±0.39, Piwil2; 1.14±0.21, Dazl; 0.65±0.25, α6 integrin; 1.26±0.53, β1 integrins; 1.18±0.65) compared to control and placental feeder cells + RA. Our results indicated that HAE and HCP followed by RA treatment were involved in human germ cell development.
Conclusion: We demonstrated that under the right conditions, hWJ-MSCs have the ability to differentiate to germ cells and this provides an excellent pattern to study infertility cause and treatment.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
325
333
https://ijbms.mums.ac.ir/article_4280_d56ce67df6b83e5cb78d7a97e0099d2d.pdf
dx.doi.org/10.22038/ijbms.2015.4280
Effects of berberine on proliferation, cell cycle distribution and apoptosis of human breast cancer T47D and MCF7 cell lines
Elmira
Barzegar
Molecular Research Lab, Department of Pharmacology and Toxicology, Tehran University of Medical Sciences, Tehran, Iran
author
Shamileh
Fouladdel
Molecular Research Lab, Department of Pharmacology and Toxicology, Tehran University of Medical Sciences, Tehran, Iran
author
Tahereh
Komeili Movahhed
Molecular Research Lab, Department of Pharmacology and Toxicology, Tehran University of Medical Sciences, Tehran, Iran
author
Shekoufeh
Atashpour
Molecular Research Lab, Department of Pharmacology and Toxicology, Tehran University of Medical Sciences, Tehran, Iran
author
Mohammad Hossein
Ghahremani
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
author
Seyed Nasser
Ostad
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
author
Ebrahim
Azizi
Molecular Research Lab, Department of Pharmacology and Toxicology, Tehran University of Medical Sciences, Tehran, Iran
author
text
article
2015
eng
Objective(s):Berberine, a naturally occurring isoquinoline alkaloid, has shown antitumor properties in some in vitro systems. But the effect of berberine on breast cancer has not yet been completely studied. In this study, we evaluated anticancer properties of berberine in comparison to doxorubicin.
Materials and Methods: The antiproliferative effects of berberine and doxorubicin alone and in combination were evaluated in T47D and MCF7 cell lines using MTT cytotoxicity assay. In addition, flow cytometry analysis was performed to evaluate the cell cycle alteration and apoptosis induction in these cell lines following exposure to berberine and doxorubicin alone and in combination.
Results: The IC50 of berberine was determined to be 25 µM after 48 hr of treatment in both cell lines but for doxorubicin it was 250 nM and 500 nM in T47D and MCF-7 cell lines, respectively. Co-treatment with berberine and doxorubicin increased cytotoxicity in T47D cells more significantly than in MCF-7 cells. Flow cytometry results demonstrated that berberine alone or in combination with doxorubicin induced G2/M arrest in the T47D cells, but G0/G1 arrest in the MCF-7 cells. Doxorubicin alone induced G2/M arrest in both cell lines. Furthermore, berberine and doxorubicin alone or in combination significantly induced apoptosis in both cell lines.
Conclusion: Berberine alone and in combination with doxorubicin inhibited cell proliferation, induced apoptosis and altered cell cycle distribution of breast cancer cells. Therefore, berberine showed to be a good candidate for further studies as a new anticancer drug in the treatment of human breast cancer.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
334
342
https://ijbms.mums.ac.ir/article_4281_03069c2fad66a0908423c6b5e8de6723.pdf
dx.doi.org/10.22038/ijbms.2015.4281
The prevention and treatment effects of egg yolk high density lipoprotein on the formation of atherosclerosis plaque in rabbits
Shima
Eftekhar
Neurocognitive Research Center and Department of Physiology, College of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Heidar
Parsaei
Department of Pharmacology, College of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Zakieh
Keshavarzi
Department of Physiology, College of Medicine, North Khorasan University of Medical Sciences, Bojnourd, Iran
author
Abbas
Tabatabaei Yazdi
Department of Pathology, College of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mosa-Al-Reza
Hadjzadeh
Neurocognitive Research Center and Department of Physiology, College of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Aliakbar
Rajabzadeh
Department of Anatomy and Cell Biology, College of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Sina
Omid Malayeri
Student Research Committee, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2015
eng
Objective(s): Atherosclerosis is the main leading cause of cardiovascular diseases. The purpose of this study was to assess the potential preventive effect of egg yolk HDL on the atherosclerosis plaque formation. Materials and Methods: Thirty rabbits were divided into five groups: A; normal diet, B; hyper-cholesterolemic diet, C; hypercholesterolemic + 400 mg/kg egg yolk HDL D; hypercholesterolemic +100 mg/kg egg yolk HDL and E; 200 mg/kg egg yolk HDL. At the end of the experiment, the lipid profiles were measured by spectrophotometric method. The histological sections of thoracic aorta also were taken and analyzed under light microscope. Results: At the end of the 2nd and the 4th weeks, there was a significant increase of cholesterol level in groups B, C, and D compared to group A (P<0.05). Following HDL treatment, triglyceride (TG) levels increased significantly versus group A and also the TG level decreased significantly in group C, D, and E versus group B (P<0.01). Egg yolk HDL significantly increased HDL-C in groups C, D, and E (P<0.01) compared to groups A and B (P<0.05). The surface area of the atherosclerotic plaque was increased significantly in group B versus group A (P<0.001). Egg yolk HDL consumption reduced the plaque size significantly (P<0.001). Conclusion: Our findings indicated that treatment with egg yolk HDL increased serum HDL-C and decreased atherosclerotic plaque size in rabbits.Thus, egg yolk HDL may be considered as an anti-atherosclerotic treatment for cardiovascular diseases.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
343
349
https://ijbms.mums.ac.ir/article_4282_0e83ea49fa897007e95328ac17444405.pdf
dx.doi.org/10.22038/ijbms.2015.4282
Immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from Brucella melitensis
Jafar
Amani
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
author
Amir
Ghasemi
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
author
Reza
Ranjbar
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
author
Mahdi
Shabani
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
author
Mahdi
Zandemami
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
author
Reza
Golmohammadi
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
author
text
article
2015
eng
Objective(s): Brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. Immunity against Brucella melitensis involves antigen-specific CD4+ and CD8+ T-cells activation and humoral immune responses. Due to negative aspects of live attenuated vaccines, much attention has been focused on finding Brucella-protective antigens to introduce them as potential subunit vaccine candidates. Materials and Methods: A chimeric gene encoding trigger factor (TF), Omp3148-74 and BP2687-111 fragments (TOB) from B. melitensis was successfully cloned, expressed in Escherichia coliBL21-DE3 and purified by Ni-NTA agarose column. Antibodies to recombinant TOB (rTOB) have been investigated in Brucella-infected human sera and a pool serum prepared from B. melitensis-vaccinated rabbits. Results: Our results showed that the immunized rabbit pool serum strongly reacted with rTOB. In addition, antibodies against rTOB were detectable in 76.5% of sera obtained from infected patients. Conclusion: These findings suggest that rTOB may provide a potential immunogenic candidate which could be considered in future vaccine studies.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
350
355
https://ijbms.mums.ac.ir/article_4283_18470303f57a9677ceb20cd27c7e6083.pdf
dx.doi.org/10.22038/ijbms.2015.4283
Neuroprotective effect of p-coumaric acid in rat model of embolic cerebral ischemia
Mustafa
Guven
Department of Neurosurgery, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey
author
Adem
Bozkurt Aras
Department of Neurosurgery, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey
author
Tarik
Akman
Department of Neurosurgery, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey
author
Halil
Murat Sen
Department of Neurosurgery, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey
author
Adile
Ozkan
Department of Neurosurgery, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey
author
Osman
Salis
Department of Medical Biochemistry, Faculty of Medicine, Ondokuz Mayıs University, Samsun, Turkey
author
Ibrahim
Sehitoglu
Department of Pathology, Faculty of Medicine, Recep Tayyip Erdoğan University, Rize, Turkey
author
Yildiray
Kalkan
Department of Histology & Embryology, Faculty of Medicine, Recep Tayyip Erdoğan University, Rize, Turkey
author
Coskun
Silan
Department of Pharmacology, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey
author
Mustafa
Deniz
Department of Physiology, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey
author
Murat
Cosar
Department of Neurosurgery, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey
author
text
article
2015
eng
Objective(s): Stroke poses a crucial risk for mortality and morbidity. Our study aimed to investigate the effect of p-coumaric acid on focal cerebral ischemia in rats.
Material and Methods: Rats were randomly divided into four groups, namely Group I (control rats), Group II (ischemia rats), Group III (6 hr ischemia + p-coumaric acid rats) and Group IV (24 hr ischemia + p-coumaric acid rats). Cerebral ischemia was induced via intraluminal monofilament occlusion model. In all groups, the brain was removed after the procedure and rats were sacrificed. Malondialdehyde, superoxide dismutase and nuclear respiratory factor-1 were measured in the ischemic hemisphere. The histopathological changes were observed in the right hemisphere within the samples. Functional assessment was performed for neurological deficit scores.
Results: Following the treatment, biochemical factors changed significantly. Histopathologically, it was shown that p-coumaric acid decreased the oxidative damage. The neurological deficit scores of p-coumaric acid-treated rats were significantly improved after cerebral ischemia.
Conclusion:Our results showed that p-coumaric acid is a neuroprotective agent on account of its strong anti-oxidant and anti-apoptotic features. Moreover, p-coumaric acid decreased the focal ischemia. Extra effort should be made to introduce p-coumaric acid as a promising therapeutic agent to be utilized for treatment of human cerebral ischemia in the future.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
356
363
https://ijbms.mums.ac.ir/article_4284_8cc68e4e116f5850243d461555c154a3.pdf
dx.doi.org/10.22038/ijbms.2015.4284
Comparison of acute effects of heroin and Kerack on sensory and motor activity of honey bees (Apis mellifera)
Majid
Hassanpour-Ezatti
Department of Biology, School of Sciences, Shahed University, Tehran, Iran
author
text
article
2015
eng
Objective(s):Previous studies demonstrated a functional similarity between vertebrate and honey bee nervous systems. The aim of the present study was to compare the effects of heroin and Iranian street Kerack, a combination of heroin and caffeine, on sensory threshold and locomotor activity in honey bees.
Materials and Methods: All drugs were given orally to honey bees 30 min before each experiment. The levels of these drugs and their metabolites in brain samples of honey bees were determined by GC/MS. The sucrose sensitivity test was used for evaluation of changes in honey bees’ sensory threshold. Following the administration of both drugs, the honey bees’ locomotor activity changes were evaluated in open fields.
Results: 6-acetylmorphine had a higher concentration in comparison with other heroin metabolites in honey bees’ brains. Concentration of the compound in the brain was directly proportional to the amount ingested. Heroin reduced the sensory threshold of honey bees, but Kerack increased it in the same doses. Locomotor activity of honey bee in open field was enhanced after the administration of both drugs. However, immobility time of honey bees was only affected by high doses of heroin.
Conclusion: Acute effects of heroin andKerack on the sensory and motor functions of honey bees were different. Findings of this research suggest that these differences originated from the activation of different neurotransmitter systems by caffeine together with activation of opioid receptors by heroin.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
364
369
https://ijbms.mums.ac.ir/article_4285_3273870ae30ff220213d4669f7e6d4e0.pdf
dx.doi.org/10.22038/ijbms.2015.4285
Protective effect of royal jelly in 2,4,6 trinitrobenzene sulfonic acid-induced colitis in rats
Turan
Karaca
University of Trakya, Faculty of Medicine, Department of Histology- Embryology, Balkan Campus, 22030, Edirne, Turkey
author
Yesim
Hulya Uz
University of Trakya, Faculty of Medicine, Department of Histology- Embryology, Balkan Campus, 22030, Edirne, Turkey
author
Selim
Demirtas
University of Trakya, Faculty of Medicine, Department of Histology- Embryology, Balkan Campus, 22030, Edirne, Turkey
author
Ihsan
Karaboga
University of Trakya, Faculty of Medicine, Department of Histology- Embryology, Balkan Campus, 22030, Edirne, Turkey
author
Guray
Can
University of Trakya, Faculty of Medicine, Department of Gastroenterolgy, Balkan Campus, 22030, Edirne, Turkey
author
text
article
2015
eng
Objective(s):In the present study, we evaluated immunological and immunomodulatory properties of royal jelly (RJ) in 2,4,6 trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats.
Materials and Methods: Eighteen adult female Wistar albino rats were divided into three groups of six animals each: a control group that received only saline solution, a TNBS-induced colitis group, and a TNBS-colitis+RJ group that received 250 mg/kg/day of RJ for seven days before the induction of colitis, following by the same treatment for an additional seven days. At the end of the experiment, cardiac blood and colon samples were obtained under deep anaesthesia from the animals in all groups. Serum interleukin-1β (IL-1β), tumour necrosis factor-alpha (TNF-α) and IL-10 levels were analyzed with an enzyme-linked immunosorbent assay (ELISA). Five-micrometre-thick sections were stained with haematoxylin-eosin (H&E) for microscopic evaluations. For immunohistochemical evaluations, the paraffin sections were stained with anti-CD3 (cluster of differentiation), anti-CD5, anti-CD8 and anti-CD45.
Results: The results showed that the oral RJ treatment inhibited proinflammatory cytokines, IL-1β and TNF-α secretion, while increasing anti-inflammatory cytokine IL-10 production in the TNBS-induced colitis+RJ group compared with the colitis group not treated with RJ. The colitis was not as severe in the colitis+RJ group, with ulcerative damage, weight loss and inflammatory scores suggesting that impaired CD3-, CD5-, CD8- and CD45-positive T cell immune responses likely mediated the anti-inflammatory effect.
Conclusion: The antioxidant and anti-inflammatory properties of RJ protected colon mucosa against TNBS-induced colitis in rats orally treated with RJ.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
370
379
https://ijbms.mums.ac.ir/article_4286_bda319ebeacacd60c57dac499c123a58.pdf
dx.doi.org/10.22038/ijbms.2015.4286
Expression analysis of CD44 isoforms S and V3, in patients with esophageal squamous cell carcinoma
Atena
Mansouri
Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
Ali Mohammad
Foroughmand
Department of Genetic, Faculty of science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Mohammad Reza
Abbaszadegan
Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
Bahram
Memar
Pathology Department, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Reihaneh
Alsadat Mahmoudian
Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mehran
Gholamin
Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2015
eng
Objective(s): CD44 is a member of the cell adhesion molecules family. Naturally, CD44S, along with CD44V3 influence the cell motility, migration, and adhesion, while in tumor cells they lead to tumor invasion, progression, and metastasis. The purpose of this research is to evaluate the CD44S and CD44V3 expression in Esophageal Squamous Cell Carcinoma (ESCC) and to reveal their correlations with clinicopathological features of patients.
Materials and Methods:Fresh tumoral and distant tumor-free esophageal tissues were obtained from 50 patients with ESCC. Using quantitative real-time PCR, the expression levels of CD44S and CD44V3 were quantified and compared in both groups of cells. The patients had not received any therapeutic interference, such as chemotherapy or radiation, prior to sampling.
Results:Significant overexpression of CD44S and CD44V3 mRNA was observed in 13 (26.0%, P=0.03) and 11 (22.0%, P=0.007) tumor specimens, respectively. The expression of the genes were significantly correlated not only with each other (P=0.0001), but also with differentiation grade of tumor (P=0.033), stage of tumor progression (P=0.003), and depth of tumor invasion (P=0.00). In addition, low level of CD44V3 mRNA expression was attended to be associated with tumor invasion.
Conclusion:There is no correlation between CD44S expression with clinicopathological features of patients; however, simultaneous expression of these genes has an important effect on tumorigenesis.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
380
384
https://ijbms.mums.ac.ir/article_4287_0ad109dd8725003a8fa62c30141ffb73.pdf
dx.doi.org/10.22038/ijbms.2015.4287
Nanolipoparticles-mediated MDR1 siRNA delivery reduces doxorubicin resistance in breast cancer cells and silences MDR1 expression in xenograft model of human breast cancer
Mahnaz
Nourbakhsh
Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mahmoud Reza
Jaafari
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Hermann
Lage
Charite´ Campus Mitte, Institute of Pathology, Charite´platz 1, D-10117 Berlin, Germany
author
Khalil
Abnous
Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Fatemeh
mosaffa
Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Ali
Badiee
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Javad
Behravan
Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2015
eng
Objective(s): P-glycoprotein (P-gp) is an efflux protein, the overexpression of which has been associated with multidrug resistance in various cancers. Although siRNA delivery to reverse P-gp expression may be promising for sensitizing of tumor cells to cytotoxic drugs, the therapeutic use of siRNA requires effective carriers that can deliver siRNA intracellularly with minimal toxicity on target cells. We investigated a special class of PEGylated lipid-based nanoparticles (NP), named nanolipoparticles (NLPs), for siRNA-mediated P-gp downregulation.
Materials and Methods: NLPs were prepared based on low detergent dialysis method. After characterization, we evaluated the effect of NLPs on siRNA delivery, and P-gp downregulation compared to oligofectamineTM (OFA) in vitro and in vivo.
Results: Our results showed a significant decrease in P-gp expression and subsequent enhancement of chemosensitivity to doxorubicin in vitro. Although the effectiveness of NLPs for in vitro siRNA delivery compared to OFA was limited, the results of in vivo studies showed noticeable effectiveness of NLPs for systemic siRNA delivery. siRNA delivery using NLPs could downregulate MDR1 in tumor cells more than 80%, while OFA had a reverse effect on MDR1 expression in vivo.
Conclusion: The results indicated that the prepared NLPs could be suitable siRNA delivery systems for tumor therapy.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
385
392
https://ijbms.mums.ac.ir/article_4288_fdb3a388d96b0a870f29bc9dc963f267.pdf
dx.doi.org/10.22038/ijbms.2015.4288
Activation of calcium/calmodulin-dependent kinase II following bovine rotavirus enterotoxin NSP4 expression
Hadi
Razavinikoo
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Hoorieh
Soleimanjahi
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Gholamreza
Haqshenas
Department of Microbiology, Faculty of Medicine, Nursing & Health Sciences, School of Biomedical Sciences, Monash University, Melbourne, Victoria, Australia
author
Taravat
Bamdad
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Ali
Ali Teimoori
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Zahra
Goodarzi
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
text
article
2015
eng
Objective(s): The rotavirus nonstructural protein 4 (NSP4) is responsible for the increase in cytoplasmic calcium concentration through a phospholipase C-dependent and phospholipase C-independent pathways in infected cells. It is shown that increasing of intracellular calcium concentration in rotavirus infected cells is associated with the activation of some members of protein kinases family such as calcium/calmodulin-dependent kinase II, which plays a crucial role in replication and pathogenesis of the virus. The aim of this study was to expression bovine rotavirus NSP4 gene in HEK293 cell and evaluation of its biological effect related to activation of calcium/calmodulin-dependent kinase II in cell culture. Materials and Methods: MA104 cells was used as a sensitive cell for propagation of virus and defined as a positive control. The NSP4 gene was amplified and inserted into an expression vector, and introduced as a recombinant plasmid into HEK293T cells. Western blot analysis was performed as a confirmation test for both expression of NSP4 protein and activation of calcium/calmodulin-dependent kinase II. Results:Expression of NSP4 and activated form of calcium/calmodulin-dependent kinase II were demonstrated by western blotting. Conclusion: It was shown that the expression of biologically active full- length NSP4 protein in HEK293T cells may be associated with some biological properties such as calcium calmodulin kinase II activation, which was indicator of rotaviruses replication and pathogenesis
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
393
397
https://ijbms.mums.ac.ir/article_4289_b5aceef970e73d8d366c386cbcf37f66.pdf
dx.doi.org/10.22038/ijbms.2015.4289
Interaction between the antioxidant activity of curcumin and cholinergic system on memory retention in adult male Wistar rats
Zeynab
Sarlak
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
Shahrbanoo
Oryan
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
Mehrnoush
Moghaddasi
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khoramabad, Iran
author
text
article
2015
eng
Objective(s): The cholinergic system plays an important role in learning and memory. This study investigated the effects of curcumin (turmeric extract) and the cholinergic system and their interaction on memory retention of passive avoidance learning in adult male Wistar rats.
Materials and Methods: At first, an injection cannula was implanted in right ventricles of the animals. One week after the surgery, the animals were trained with a shuttle box set up. Post-training, injections were performed in all experiments. Administration of curcumin increased memory retention. Also administrations of nicotine and pilocarpine, the cholinergic receptor agonists, increased memory retention, while it is decreased by succinylcholine and scopolamine, the cholinergic receptor antagonists. Then co-administration of curcumin and cholinergic drugs were performed. Intraperitoneal and intracerebroventricular injections were applied for the curcumin and cholinergic drugs, respectively.
Results: Co-administration of curcumin (45 mg/kg) with a low dose of nicotine (0.1 µg/rat) or pilocarpine (0.5 µg/rat) increased memory retention significantly. Effects of succinylcholine (0.01, 0.1 and 0.5 µg/rat) or scopolamine (0.1, 1 and 5 µg/rat) were attenuated by curcumin markedly (45 mg/kg).
Conclusion: The results suggest that curcumin has a close interaction with cholinergic system in memory retention process.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
398
403
https://ijbms.mums.ac.ir/article_4290_ecf38f2c7813043988218033d33a88ec.pdf
dx.doi.org/10.22038/ijbms.2015.4290
Modulation of IKKβ/NF-κB and TGF-β1/Smad via Fuzheng Huayu recipe involves in prevention of nutritional steatohepatitis and fibrosis in mice
Rong-Qi
Wang
Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei Medical University, Shijiazhuang, China
author
Hong
Mei Mi
Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei Medical University, Shijiazhuang, China
author
Hui
Li
Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei Medical University, Shijiazhuang, China
author
Su
Xian Zhao
Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei Medical University, Shijiazhuang, China
author
Yan
Hong Jia
Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei Medical University, Shijiazhuang, China
author
Yue-Min
Nan
Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei Medical University, Shijiazhuang, China
author
text
article
2015
eng
Objective(s):Fuzheng Huayu recipe (FZHY) exerts significant protective effects against liver fibrosis by strengthening the body’s resistance and removing blood stasis. However, the molecular mechanisms through which FZHY affects liver fibrosis are still unclear. In this study, we examined the expression levels of factors involved in the inhibitor κB kinase-β (IKK-β)/nuclear factor-κB (NF-κB) and transforming growth factor beta 1 (TGF-β1)/Smad signaling pathways to elucidate whether FZHY could attenuate nutritional steatohepatitis and fibrosis in mice.
Materials and Methods: C57BL/6J mice were fed with methionine-choline deficient (MCD) diet for 8 weeks to induce fibrotic steatohepatitis. FZHY and/or heme oxygenase-1 (HO-1) chemical inducer (hemin) were administered to mice. The effects of FZHY alone and in combination with hemin were assessed by comparing the severity of hepatic injury, activation of hepatic stellate cells (HSCs), and the expression of oxidative stress, inflammation and fibrogenesis related genes.
Results: Administration of FZHY, hemin and FZHY plus hemin significantly ameliorated liver injury. Additionally, our analysis indicated that administration of these agents significantly attenuated oxidative stress, downregulated the expression of pro-inflammatory and pro-fibrotic genes, including IKK-β, NF-κB, monocyte chemoattractant protein-1 (MCP-1), α-smooth muscle actin (α-SMA), TGF-β1, Smad3 and Smad4, and upregulated the expression of the antifibrogenic gene Smad7 (P< 0.001).
Conclusion: FZHY-containing therapies prevented nutritional steatohepatitis and fibrosis through modulating the expression of factors associated with the IKKβ/NF-κB and TGF-β1/Smad signaling pathways and oxidative stress related genes.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
404
411
https://ijbms.mums.ac.ir/article_4291_76a13506e5d9d5514391115facdab13a.pdf
dx.doi.org/10.22038/ijbms.2015.4291
Nephroprotective potential of Graptophyllum pictum against renal injury induced by gentamicin
Keloth
Kaitheri Srinivasan
Department of Chemistry, Shri Madhwa Vadiraja Institute of Technology and Management, Udupi- 574115, India
author
Jessy
Elizabeth Mathew
Department of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal-576104, Karnataka, India
author
Kerryn
Joseph A.D’Silva
Department of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal-576104, Karnataka, India
author
Richard
Lobo
Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal-576104, Karnataka, India
author
Nimmy
Kumar
Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal-576104, Karnataka, India
author
text
article
2015
eng
Objective(s):To evaluate the effect of Graptophyllum pictum on lipid peroxidation and tissue antioxidant enzymes in liver and kidney of gentamicin induced nephrotoxic rats.
Materials and Methods: Animals were grouped into 6: Group 1 received gum acacia, Group 2 received G. pictum ethanol extract (300 mg/kg), Group 3 received gentamicin, Groups 4, 5, 6 received gentamicin along with G. pictum at 300, 150, 75 mg/kg, respectively. Nephroprotective activity was evaluated by measuring thiobarbituric acid-reactive substances (TBARS), biochemical markers Glutathione (GSH), Glutathione-S Transferase(GST), Superoxide dismutase (SOD), Catalase (CAT), serum urea and creatinine levels.
Results: Results obtained showed that gentamicin induced nephrotoxic rats exhibited lower activities of biochemical markers and raised levels of TBARS, serum creatinine and urea. Remarkably, after treatment with G. pictum extract, anomalous levels of biochemical markers, lipid peroxidation and serum creatinine were returned to normal.
Conclusion: The results propose that G. pictum has nephroprotective effects, and can be a promising natural source against gentamicin induced nephrotoxicity.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
412
416
https://ijbms.mums.ac.ir/article_4292_cd112c877462bad0d7184288359719f3.pdf
dx.doi.org/10.22038/ijbms.2015.4292
Hypoglycemic and hypolipidemic activities of Salvia hydrangea in streptozotocin-induced diabetes in rats
Ali
Zarei
Department of Biology, Damghan Branch, Islamic Azad University, Semnan, Iran
author
Gholamhasan
Vaezi
Department of Biology, Damghan Branch, Islamic Azad University, Semnan, Iran
author
Ali Akbar
Malekirad
Department of Biology, Payame Noor University, Iran
author
Mohammad
Abdollahi
Faculty of Pharmacy, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran
author
text
article
2015
eng
Objective(s): This study was to investigate the potential anti-diabetic effects of alcoholic extract of Salvia hydrangea in rats.
Materials and Methods:Thirty five male Wistar rats were divided into five groups namely non-diabetic control, diabetic control, and three experimental diabetic that received either Salvia hydrangea extract for 21 days at the doses of 100 and 200 or glibenclamide at the dose of 10 mg/kg through gavage feeding. To induce diabetes, streptozotocin was injected intraperitoneally.
Results:Insulin and HDL levels in the group receiving the high dose of the extract showed significant increase, whereas the amount of cholesterol in rats that received glibenclamide and the extract showed a significant decrease as compared to the diabetic control group (P<0.05). The blood glucose levels showed significant reduction in all experimental groups (P<0.05).
Conclusion: Consumption of the extract of the aerial parts of S. hydrangea which reduces blood fat and increases insulin may have beneficial effects on the symptoms of diabetes and hyperlipidemia.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
18
v.
4
no.
2015
417
422
https://ijbms.mums.ac.ir/article_4293_613ae71b61d6ab5728457a06d2077c49.pdf
dx.doi.org/10.22038/ijbms.2015.4293