@article { author = {Gilany, Kambiz and Mohamadkhani, Ashraf and Chashmniam, Saeed and Shahnazari, Parisa and Amini, Mehdi and Arjmand, Babak and Malekzadeh, Reza and Nobakht Motlagh Ghoochani, Bibi Fatemeh}, title = {Metabolomics analysis of the saliva in patients with chronic hepatitis B using nuclear magnetic resonance: a pilot study}, journal = {Iranian Journal of Basic Medical Sciences}, volume = {22}, number = {9}, pages = {1044-1049}, year = {2019}, publisher = {Mashhad University of Medical Sciences}, issn = {2008-3866}, eissn = {2008-3874}, doi = {10.22038/ijbms.2019.36669.8733}, abstract = {Objective(s): Hepatitis B virus infection causes chronic disease such as cirrhosis and hepatocellular carcinoma. The metabolomics investigations have been demonstrated to be related to pathophysiologic mechanisms in many disorders such as hepatitis B infection. The aim of this study was to investigate the saliva metabolic profile of patients with chronic hepatitis B infection and to identify underlying mechanisms as well as potential biomarkers associated with the disease. Materials and Methods: Saliva from 16 healthy subjects and 20 patients with chronic hepatitis B virus were analyzed by nuclear magnetic resonance (NMR). Then, multivariate statistical analysis was performed to identify discriminative metabolites between two groups. Results: A set of metabolites were detected, including propionic acid, putrescine, acetic acid, succinic acid, tyrosine, lactic acid, butyric acid, pyruvic acid, 4-pyridoxic acid and 4-hydroxybenzoic acid, which in combination with one another could accurately distinguish patients from healthy controls. Our results clearly demonstrated altered metabolites are involved in nine metabolic pathways. Conclusion: Metabolomics has the potential to be considered as a novel clinical tool for hepatitis B diagnosis while contributing to a comprehensive understanding of disease mechanisms.}, keywords = {Diagnostic biomarkers,Hepatitis B virus,Metabolomics,NMR,Saliva}, url = {https://ijbms.mums.ac.ir/article_13244.html}, eprint = {https://ijbms.mums.ac.ir/article_13244_754d1d07ca4716768520879065003ea2.pdf} }