@article { author = {Sahab Negah, Sajad and Aligholi, Hadi and Khaksar, Zabihollah and Kazemi, Hadi and Modarres Mousavi, Sayed Mostafa and Safahani, Maryam and Barati Dowom, Parastoo and Gorji, Ali}, title = {Survival, proliferation, and migration of human meningioma stem-like cells in a nanopeptide scaffold}, journal = {Iranian Journal of Basic Medical Sciences}, volume = {19}, number = {12}, pages = {1271-1278}, year = {2016}, publisher = {Mashhad University of Medical Sciences}, issn = {2008-3866}, eissn = {2008-3874}, doi = {10.22038/ijbms.2016.7907}, abstract = {Objective(s): In order to grow cells in a three-dimensional (3D) microenvironment, self-assembling peptides, such as PuraMatrix, have emerged with potential to mimic the extracellular matrix. The aim of the present study was to investigate the influence of the self-assembling peptide on the morphology, survival, proliferation rate, migration potential, and differentiation of human meningioma stem-like cells (hMgSCs). Materials and Methods: The efficacy of a novel method for placing hMgSCs in PuraMatrix (the injection approach) was compared to the encapsulation and surface plating methods. In addition, we designed a new method for measurement of migration distance in 3D cultivation of hMgSCs in PuraMatrix. Results: Our results revealed that hMgSCs have the ability to form spheres in stem cell culture condition. These meningioma cells expressed GFAP, CD133, vimentin, and nestin. Using the injection method, a higher proliferation rate of the hMgSCs was observed after seven days of culture. Furthermore, the novel migration assay was able to measure the migration of a single cell alone in 3D environment. Conclusion: The results indicate the injection method as an efficient technique for culturing hMgSCs in PuraMatrix. Furthermore, the novel migration assay enables us to evaluate the migration of hMgSCs.}, keywords = {brain tumor,Cell culture,Cell migration,RADA16-I,Stem cells}, url = {https://ijbms.mums.ac.ir/article_7907.html}, eprint = {https://ijbms.mums.ac.ir/article_7907_737e0b17264a1097d43c4cbaf1e1587e.pdf} }