@article { author = {Zhuo, Song-Ming and Li, Si-Cong and Lin, Yong-Qun and Yu, Hai-Bin and Li, Na}, title = {The effects of anti-Fas ribozyme on T lymphocyte apoptosis in mice model with chronic obstructive pulmonary disease}, journal = {Iranian Journal of Basic Medical Sciences}, volume = {20}, number = {10}, pages = {1102-1108}, year = {2017}, publisher = {Mashhad University of Medical Sciences}, issn = {2008-3866}, eissn = {2008-3874}, doi = {10.22038/ijbms.2017.9367}, abstract = {Objective(s): In this study, we aimed to investigate the effects of anti-Fas ribozyme on the apoptosis of T lymphocytes (T cells) in mice model with chronic obstructive pulmonary disease (COPD). Materials and Methods: Male 6-week-old C57BL/6 mice were used to establish the COPD model by exposure to cigarette smoke. The COPD mice were sacrificed for spleen dissection and T cell isolation. T cells were randomly divided into four groups (n=10 per group). Group A was used as the control. B, C, and D groups were transfected with empty lentivirus, anti-Fas ribozyme, and an anti-Fas ribozyme mutant, respectively. The expression of Fas mRNA and protein in the T cells were evaluated using qPCR and Western blot, respectively. Flow cytometry was used to evaluate the apoptosis of CD4+T cells and calculate the ratio of CD4+ to CD8+ T cells (CD4+/CD8+). Results: Anti-Fas ribozyme significantly inhibited the expression of Fas in the T cells of COPD mice. In addition, the number of apoptotic CD4+ T cells and CD4+/CD8+of the C and D groups were significantly lower and higher than those of group A, respectively (P<0.05). The apoptotic CD4+T cells and CD4+CD8+of the C group were significantly lower and higher than those of group D, respectively (P<0.05). Conclusion: Anti-Fas ribozyme significantly inhibited the expression of Fas, increased CD4+/ CD8+, and inhibited the apoptosis of T cells in COPD mice.}, keywords = {Apoptosis,Anti-Fas ribozyme,COPD mice model,Fas,T cells}, url = {https://ijbms.mums.ac.ir/article_9367.html}, eprint = {https://ijbms.mums.ac.ir/article_9367_fb6c9ec7d98c69b0929700f8e584a8a1.pdf} }