eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
579
584
10.22038/ijbms.2016.7124
7124
Identification of Mycobacterium tuberculosis adherence-mediating components: a review of key methods to confirm adhesin function
Saiyur Ramsugit
saiyur.r@gmail.com
1
Manormoney Pillay
pillayc@ukzn.ac.za
2
Medical Microbiology and Infection Control, University of KwaZulu-Natal, Durban, South Africa
Medical Microbiology and Infection Control, University of KwaZulu-Natal, Durban, South Africa
Anti-adhesion therapy represents a potentially promising avenue for the treatment and prevention of tuberculosis in a post-antibiotic era. Adhesins are surface-exposed microbial structures or molecules that enable pathogenic organisms to adhere to host surfaces, a fundamental step towards host infection. Although several Mycobacterium tuberculosis adhesins have been identified, it is predicted that numerous additional adherence-mediating components contribute to the virulence and success of this pathogen. Significant further research to discern and characterize novel M. tuberculosis adhesins is, therefore, required to gain a holistic account of M. tuberculosis adhesion to the host. This would enable the identification of potential drug and vaccine targets for attenuating M. tuberculosis adherence and infectivity. Several methods have been successfully applied to the study and identification of M. tuberculosis adhesins. In this manuscript, we review these methods, which include adherence assays that utilize wild-type and gene knockout mutant strains, epitope masking and competitive inhibition analyses, extracellular matrix protein binding assays, microsphere adhesion assays, M. tuberculosis auto-aggregation assays, and in silico analyses.
https://ijbms.mums.ac.ir/article_7124_9dcfc5e3006754b2c063e0b571fb7607.pdf
Adhesin,
Mycobacterium –
tuberculosis ,
Pathogenesis,
Virulence factor
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
585
593
10.22038/ijbms.2016.7125
7125
Protective effect of curcumin in fructose-induced metabolic syndrome and in streptozotocin-induced diabetes in rats
Adriana Bulboacă
adriana_bulboaca@yahoo.com
1
Sorana D Bolboacă
sbolboaca@gmail.com
2
Soimiţa Suci
3
Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca, Department of Pathophysiology, Victor Babeş Street, no. 2-4, 400012 Cluj-Napoca, Romania
Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca, Department of Medical Informatics and Biostatistics, Louis Pasteur Street, no. 6, 400349 Cluj-Napoca, Romania
Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca, Department of Physiology, Clinicilor Street, no. 1, 400006 Cluj-Napoca, Romania
Objective: The aim of this study was to investigate the effect of pre-treatment with curcumin on metabolic changes induced by two different pathophysiological mechanisms in rats (fructose diet and streptozotocin (STZ)-induced diabetes mellitus). Materials and Methods: Five groups with 10 rats per group were investigated: control group (healthy rats), fructose diet groups without any pre-treatment (FD), fructose diet groups with curcumin pre-treatment (FDC), STZ-induced diabetes mellitus without any pre-treatment (SID) and STZ-induced diabetes mellitus with curcumin pre-treatment (SIDC). Systolic blood pressure, and several metabolic and oxidative stress parameters were assessed. Results: Systolic blood pressure significantly increased in all groups compared with control group (P<0.001), with significantly lower values on groups with curcumin pre-treatment compared with the group without any pre-treatment and same inducement (FDS vs. FD P<0.0001, SIDC vs. SID P<0.0001). High-density lipoprotein (HDL)-cholesterol was significantly lower in all groups compared with control group (P<0.05) while triglycerides (P<0.05), aspartate aminotransferase (AST, P<0.0001) and alanine aminotransferase (ALT, P<0.0001) were significantly higher. Within the group with same induction, curcumin pre-treatment significantly improved metabolic (total cholesterol, glycaemia, triglycerides, AST, ALT; P<0.05) and oxidative stress parameters (total oxidative status (NOx), Thiol, and malondialdehyde (MDA), P<0.02) compared to untreated groups. Conclusion: The pre-treatment with curcumin in our experimental models significantly improved metabolic (total cholesterol, triglycerides, AST and ALT) as well as oxidative stress parameters (MDA, NOx, and Thiol) in both fructose diet and in STZ-induced diabetes in rats. These properties of curcumin may serve to improve the metabolic and oxidative stress conditions in patients with these pathological features.
https://ijbms.mums.ac.ir/article_7125_60dd4ad0728287510abcde49bd0d6542.pdf
Curcumin,
Diabetes mellitus,
Fructose,
Metabolic syndrome Oxidative stress,
Streptozotocin–induced diabetes
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
594
600
10.22038/ijbms.2016.7126
7126
Swimming exercise attenuates psychological dependence and voluntary methamphetamine consumption in methamphet- amine withdrawn rats
Fatemeh Damghani
damghanifateme@yahoo.com
1
Imanollah Bigdeli
ibigdeli@um.ac.ir
2
Hossein Miladi-Gorji
miladi331@yahoo.com
3
Atefeh Fadaei
fadaei.0057@yahoo.com
4
Faculty of Psychology and Educational Sciences, University of Semnan, Semnan, Iran
Faculty of Educational Sciences and Psychology, Ferdowsi University of Mashhad, Mashhad, Iran
Laboratory of Animal Addiction Models, Research Center and Department of Physiology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
Faculty of Psychology and Educational Sciences, University of Semnan, Semnan, Iran
Objective(s): This study evaluated the effect of swimming exercise during spontaneous methamphetamine (METH) withdrawal on the anxiety, depression, obsessive-compulsive disorder (OCD) and voluntary METH consumption in METH-dependent rats. Materials and Methods: Male Wistar rats were repeatedly administered with bi-daily doses of METH (2 mg/kg, subcutaneous) over a period of 14 days. Exercised rats were submitted to swimming sessions (45 min/day, five days per week, for 14 days) during spontaneous METH-withdrawal. Then, all animals were tested for the assessment of anxiety by using the elevated plus-maze (EPM), the grooming behaviors (OCD), and depression using forced swimming test (FST) and voluntary METH consumption using a two-bottle choice (TBC) paradigm for the assessment of craving. Results: The results showed that the swimmer METH-withdrawn rats exhibited an increase in EPM open arm time and entries and a reduction of immobility and grooming behaviors compared with the sedentary METH groups. Also, voluntary METH consumption was less in the swimmer METH-withdrawn rats than the sedentary METH groups throughout 5–8 days. Conclusion: This study showed that regular swimming exercise reduced voluntary METH consumption in animal models of craving by reducing anxiety, OCD, and depression in the METH-withdrawn rats. Thus, physical training may be ameliorating some of the withdrawal behavioral consequences of METH.
https://ijbms.mums.ac.ir/article_7126_fef88e2abc611c46919cdc05f1270563.pdf
Anxiety,
Craving,
Depression Methamphetamine- abstinance,
Obsessive-compulsive disorder,
Swimming
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
601
607
10.22038/ijbms.2016.7127
7127
Transcriptional regulation of E-cadherin and oncoprotein E7 by valproic acid in HPV positive cell lines
Ebrahim Faghihloo
1
abolfazl akbari
2
Fatemeh Adjaminezhad-Fard
adjaminezhad-fard@tums.ac.ir
3
Talat Mokhtari-Azad
mokhtari@sina.tums.ac.ir
4
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Objective(s): Valproic acid (VPA) has proven to be as one of the most promising useful drug with anticancer properties.In this study, we investigate the VPA effects on E-cadherin expression in HeLa, TC1, MKN45, and HCT116 cell lines. This study assesses the effects of VPA on human papillomavirus E7 expression in HPV positive cell lines.
Materials and Methods: Cell lines were treated by2 mmol/l VPA and expression of E-cadherin and E7 was analyzed by quantitative real-time PCR. Student’s t test and ANOVA were used to determine changes in expression levels.
Results:The results revealed that mean of E-cadherin expression is increased by VPA 1.8 times in HCT116 and MKN45 cell lines, also the mean of E-cadherin mRNA levels is up-regulated 2.9 times in HeLa and TC1 cell lines. So, E-cadherin augmentation induced by VPA in HeLa and TC-1, HPV positive cell lines, is higher than HPV negative cell lines MKN45 and HCT116. The mean of HPV E7 expression is decreased by VPA, 4.6 times in in HeLa and TC-1 cell lines.
Conclusion: This study demonstrates that re-expression of E-cadherin by VPA in HPV positive cell lines is more than HPV negative cell lines. Whereas, HPV E7 reduces the expression of E-cadherin, reduction of HPV E7 expression by VPA is related to more augmentation of E-cadherin in HPV positive cell lines. So, this study demonstrates that VPA has more anticancer properties in HPV positive cell lines, and could potentially be a promising candidate for cervical cancer treatment.
https://ijbms.mums.ac.ir/article_7127_b9e02b0a48a303e2eb4d9a1470cad79a.pdf
Cervical Cancer
E-cadherin
HPV
Valproic acid
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
608
614
10.22038/ijbms.2016.7128
7128
Effect of magnesium sulfate on hyperthermia and pentylen-tetrazol-induced seizure in developing rats
Maryam Ghadimkhani
1
Ehsan Saboory
saboory@zums.ac.ir
2
Shiva Roshan-Milani
shivamilani@umsu.ac.ir
3
Sedra Mohammdi
4
Yousef Rasmi
yr1350@yahoo.com
5
Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran
Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Objective(s): Febrile seizures (FS) are the most common type of convulsive events among children. Its prevalence has been estimated to be 2-5% in children between 3 months and 5 years old. Also, blood and CSF magnesium levels have been demonstrated to be reduced in children with FS. This study investigates the effect of MgSo4 pretreatment on the behaviors caused by hyperthermia (HT) and effect of these two on pentylen-tetrazol (PTZ)-induced seizure later in life.
Materials and Methods: Thirty two Wistar rats were assigned to 2 groups: saline-hyperthermia-pentylentetrazol (SHP) and magnesium-hyperthermia-pentylentetrazol (MHP). In both groups, HT was induced at the age of 18-19 days old. Before the HT, MHP group received MgSo4 and SHP group received normal saline intraperitoneally (IP). Behaviors of the rats were recorded during the HT. Then, in half of each group (n=8) at the age of 25-26 days old and in other half at the age of 78-79 days, seizure was induced by PTZ.
Results: The HT successfully caused convulsive behaviors in the rats and pretreatment with MgSo4 before HT attenuated HT-induced convulsive behaviors. PTZ-induced seizures a week later was more severe than those of 2 months later.
Conclusion: It can be concluded that pretreatment with MgSO4 inhibits HT-induced seizure and, in a long run, this intervention reduced PTZ-induced seizure later in life.
https://ijbms.mums.ac.ir/article_7128_b61a5805806ac856cb834f25246fb3f1.pdf
Hyperthermia
Later in life
MgSO4
PTZ
Seizure
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
615
623
10.22038/ijbms.2016.7129
7129
Effect of antihypertensive agents - captopril and nifedipine - on the functional properties of rat heart mitochondria
Ivana Kancirová
usrdivka@savba.sk
1
Magdaléna Jašová
jasovam@gmail.com
2
Iveta Waczulíková
waczulikova@gmail.com
3
Táňa Ravingerová
usrdravi@savba.sk
4
Attila Ziegelhöffer
5
Miroslav Ferko
usrdmife@savba.sk
6
Department of Biochemistry, Institute for Heart Research, Centre of Excellence SAS NOREG, Slovak Academy of Sciences, Bratislava, Slovakia
Department of Biochemistry, Institute for Heart Research, Centre of Excellence SAS NOREG, Slovak Academy of Sciences, Bratislava, Slovakia
Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics, and Informatics, Comenius University, Bratislava, Slovakia
Department of Physiology, Institute for Heart Research, Centre of Excellence SAS NOREG, Slovak Academy of Sciences, Bratislava, Slovakia
Department of Biochemistry, Institute for Heart Research, Centre of Excellence SAS NOREG, Slovak Academy of Sciences, Bratislava, Slovakia
Department of Biochemistry, Institute for Heart Research, Centre of Excellence SAS NOREG, Slovak Academy of Sciences, Bratislava, Slovakia
Objective(s): Investigation of acute effect on cellular bioenergetics provides the opportunity to characterize the possible adverse effects of drugs more comprehensively. This study aimed to investigate the changes in biochemical and biophysical properties of heart mitochondria induced by captopril and nifedipine antihypertensive treatment. Materials and Methods: Male, 12-week-old Wistar rats in two experimental models (in vivo and in vitro) were used. In four groups, the effects of escalating doses of captopril, nifedipine and combination of captopril + nifedipine added to the incubation medium (in vitro) or administered per os to rat (in vivo) on mitochondrial ATP synthase activity and membrane fluidity were monitored. Results: In the in vitro model we observed a significant inhibitory effect of treatment on the ATP synthase activity (P<0.05) with nonsignificant differences in membrane fluidity. Decrease in the value of maximum reaction rate Vmax(P<0.05) without any change in the value of Michaelis-Menten constant Km, indicative of a noncompetitive inhibition, was presented. At the in vivo level, we did not demonstrate any significant changes in the ATP synthase activity and the membrane fluidity in rats receiving captopril, nifedipine, and combined therapy. Conclusion: In vitro kinetics study revealed that antihypertensive drugs (captopril and nifedipine) directly interact with mitochondrial ATP synthase. In vivo experiment did not prove any acute effect on myocardial bioenergetics and suggest that drugs do not enter cardiomyocyte and have no direct effect on mitochondria.
https://ijbms.mums.ac.ir/article_7129_fb8c89258d7bf6fb33998a384e9c72d7.pdf
Captopril
Heart
Membrane fluidity
Mitochondria
Mitochondrial proton-translocating ATPases
Nifedipine
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
624
631
10.22038/ijbms.2016.7130
7130
Effect of ischemic preconditioning on the expression of c-myb in the CA1 region of the gerbil hippocampus after ischemia/reperfusion injury
Hui Young Lee
1
Hyun-Jin Tae
anatotae@gmail.com
2
Geum-Sil Cho
anagscho@shinpoog.ac.kr
3
In Hye Kim
inhye1987@naver.com
4
Jeong Hwi Cho
5
Joon Ha Park
6
Ji Hyeon Ahn
7
Bai Hui Chen
8
Bich-Na Shin
tlsqlcsk21@nate.com
9
Moo-Ho Won
mhwon@kangwon.ac.kr
10
Chan Woo Park
11
Jun Hwi Cho
12
Jeong Yeol Seo
13
Jae-Chul Lee
anajclee@kangwon.ac.kr
14
Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea
Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon 200-702, South Korea
Pharmacology & Toxicology Department, Shinpoong Pharmaceutical Co., Ltd., Ansan 425-100, South Korea
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea
Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon 200-702, South Korea
Department of Physiology, College of Medicine, Hallym University, Chuncheon 200-702, South Korea
Department of Physiology, College of Medicine, Hallym University, Chuncheon 200-702, South Korea
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea
Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea
Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea
Department of Emergency Medicine, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, Chuncheon 200-702, South Korea
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea
Objective(s): In the present study, we investigated the effect of ischemic preconditioning (IPC) on c-myb immunoreactivity as well as neuronal damage/death after a subsequent lethal transient ischemia in gerbils.
Materials and Methods: IPC was subjected to a 2 min sublethal ischemia and a lethal transient ischemia was given 5 min transient ischemia. The animals in all of the groups were given recovery times of 1 day, 2 days and 5 days and we examined change in c-myb immunoreactivity as well as neuronal damage/death in the hippocampus induced by a lethal transient ischemia.
Results:A lethal transient ischemia induced a significant loss of cells in the stratum pyramidale (SP) of the hippocampal CA1 region at 5 days post-ischemia, and this insult showed that c-myb immunoreactivity in cells of the SP of the CA1 region was significantly decreased at 2 days post-ischemia and disappeared at 5 days post-ischemia. However, IPC effectively prevented the neuronal loss in the SP and showed that c-myb immunoreactivity was constitutively maintained in the SP after a lethal transient ischemia.
Conclusion: Our results show that a lethal transient ischemia significantly decreased c-myb immunoreactivity in the SP of the CA1 region and that IPC well preserved c-myb immunoreactivity in the SP of the CA1 region. We suggest that the maintenance of c-myb might be related with IPC-mediated neuroprotection after a lethal ischemic insult.
https://ijbms.mums.ac.ir/article_7130_abf6b260ad1d4b6f4533bbdf5bd04588.pdf
c-myb
Cells in stratum pyramidale
Delayed neuronal death
Ischemic preconditioning
Ischemia-reperfusion
protection
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
632
637
10.22038/ijbms.2016.7131
7131
Time-dependent changes of autophagy and apoptosis in lipopolysaccharide-induced rat acute lung injury
Li Lin
1
Lijun Zhang
2
Liangzhu Yu
3
Lu Han
4
Wanli Ji
5
Hui Shen
6
Zhenwu Hu
huzhenwu002@hotmail.com
7
School of Basic Medicine, Hubei University of Science and Technology, Xianning 437100, P.R. China
School of Basic Medicine, Hubei University of Science and Technology, Xianning 437100, P.R. China
School of Basic Medicine, Hubei University of Science and Technology, Xianning 437100, P.R. China
School of Basic Medicine, Hubei University of Science and Technology, Xianning 437100, P.R. China
School of Basic Medicine, Hubei University of Science and Technology, Xianning 437100, P.R. China
School of Basic Medicine, Hubei University of Science and Technology, Xianning 437100, P.R. China
School of Basic Medicine, Hubei University of Science and Technology, Xianning 437100, P.R. China
Objective(s): Abnormal lung cell death including autophagy and apoptosis is the central feature in acute lung injury (ALI). To identify the cellular mechanisms and the chronology by which different types of lung cell death are activated during lipopolysaccharide (LPS)-induced ALI, we decided to evaluate autophagy (by LC3-II and autophagosome) and apoptosis (by caspase-3) at different time points after LPS treatment in a rat model of LPS-induced ALI.
Materials and Methods: Sprague-Dawley rats were randomly divided into two groups: control group and LPS group. ALI was induced by LPS intraperitoneal injection (3 mg/kg). The lung tissues were collected to measure lung injury score by histopathological evaluation, the protein expression of LC3-II and caspase-3 by Western blot, and microstructural changes by electron microscopy analysis.
Results: During ALI, lung cell death exhibited modifications in the death process at different stages of ALI. At early stages (1 hr and 2 hr) of ALI, the mode of lung cell death started with autophagy in LPS group and reached a peak at 2 hr. As ALI process progressed, apoptosis was gradually increased in the lung tissues and reached its maximal level at later stages (6 hr), while autophagy was time-dependently decreased.
Conclusion:These findings suggest that activated autophagy and apoptosis might play distinct roles at different stages of LPS-induced ALI. This information may enhance the understanding of lung pathophysiology at the cellular level during ALI and pulmonary infection, and thus help optimize the timing of innovating therapeutic approaches in future experiments with this model.
https://ijbms.mums.ac.ir/article_7131_72e52b93e619de18ab5f942496a28d16.pdf
Acute lung injury
Apoptosis
Autophagy
Lipopolysaccharide
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
638
645
10.22038/ijbms.2016.7132
7132
Study of chondrogenic potential of stem cells in co-culture with chondrons
Parisa Nikpou
pnikpou@gmail.com
1
Daryoush Mohammad Nejad
2
Hajar Shafaei
shafaei49@gmail.com
3
Leila Roshangar
4
Nasser Samadi
nsamadi@ualberta.ca
5
Amir Mohammad Navali
amir_navali@yahoo.com
6
Ali Reza Sadegpour
7
Dariush Shanehbandi
dariush_ shanehbandi@yahoo.com
8
Jafar Soleimani Rad
soleimani@tbzemed.ac.ir
9
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Biochemistry and Laboratory Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Orthopedy, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Orthopedy, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Immunolog, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Objective(s): Three-dimensional biomimetic scaffolds have widespread applications in biomedical tissue engineering due to similarity of their nanofibrous architecture to native extracellular matrix. Co-culture system has stimulatory effect on chondrogenesis of adult mesenchymal stem cells. This work presents a co-culture strategy using human articular chondrons and adipose-derived stem cells (ASCs) from infrapatellar fat pad (IPFP) for cartilage tissue production. Materials and Methods: Isolated stem cells were characterized by flowcytometry. Electrospun and polycaprolactone (PCL) scaffolds (900 nm fiber diameter) was obtained from Bon Yakhteh (Tehran- Iran) and human infrapatellar fat pad-derived stem cells (IPFP-ASCs) were seeded on them. IPFP- ASCs on scaffolds were co-cultured with articular chondrons using transwell. After 21 day, chondrogenic differentiation of stem cell was evaluated by determining the genes expression of collagen2, aggrecan and Indian hedgehog using real- time RT-PCR. Results: Genes expression of collagen2, aggrecan by IPFP-ASCs did not alter significantly in comparison with control group. Howevers, expression of Indian hedgehog decreased significantly compared to control group (P˂ 0.05). Conclusion: These findings indicate that chondrons obtained from osteoarthritic articular cartilage did not stimulate chondrogenic differentiation of IPFP-ASCs in co-culture.
https://ijbms.mums.ac.ir/article_7132_2606d4c6a08fd8ae8de07aa5cb0d2bea.pdf
Chondron
Co-culture
Nanofiber
Poly-e-caprolactone scaffold
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
646
652
10.22038/ijbms.2016.7133
7133
Protective effect of pentoxifylline on male Wistar rat testicular germ cell apoptosis induced by 3,4-methylenedioxymeth amphetamine
Mahnaz Nouri
dr.nouri@yahoo.com
1
Shabnam Movassaghi
shmovasaghi@iautmu.ac.ir
2
Alireza foroumadi
3
Mansooreh Soleimani
mansoorehsoleimani@gmail.com
4
Zahra Nadia Sharifi
zsharifi@iautmu.ac.ir
5
Department of Medical Sciences, Shahrood Branch, Islamic Azad University, Shahrood, Iran
Department of Anatomy, School of Medicine, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Research Center, Tehran University of Medical Sciences, Tehran, Iran
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
Department of Anatomy, School of Medicine, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Objective(s): 3, 4-methylenedioxymethamphetamine (MDMA) one of the methamphetamine derivatives that affect the reproductive system, has not been well understood. Many young people are consumers of drugs such as MDMA that can affect their reproductive capability. Apoptosis is the main mechanism for male infertility. Pentoxifylline (PTX) increases cAMP intracellularly and reduces tumor necrosis factor-α.
This study aimed to investigate the protective effect of PTX administration in MDMA-induced apoptosis in testes of male Wistar rats.
Materials and Methods: Thirty male Wistar rats weighing 250–300 g were randomly divided into five groups: control group (without any intervention), group receiving 7.5 mg/kg MDMA three times every two hours for one day, first experimental group receiving 100 mg/kg PTX just at the time of third injection of MDMA, second experimental group receiving 100 mg/kg PTX a week before MDMA administration, and the vehicle group, which received MDMA+saline. Two weeks later, testes were removed and prepared for H&E staining, TUNEL and Western blot techniques.
Results: There was a significant decrease of the score in the MDMA group compared with the control group. In first and second experimental groups, the quality of seminiferous epithelium was improved compared with the MDMA group. The number of TUNEL-positive cells/tubule increased in MDMA and vehicle groups, which is decreased by administration of PTX before MDMA. Expression of active caspase-3 significantly increased in MDMA group, which is significantly decreased by administration of PTX before MDMA.
Conclusion: PTX can significantly reduce the severity of lesions in the testes following administration of MDMA.
https://ijbms.mums.ac.ir/article_7133_780dd9e18731dfb66954e222d5cd6630.pdf
Apoptosis
Pentoxifylline
Testes
3, 4-methylene-dioxymethamphetamine
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
653
661
10.22038/ijbms.2016.7134
7134
Histomorphometric evaluation of treatment of rat azoosper-mic seminiferous tubules by allotransplantation of bone marrow-derived mesenchymal stem cells
Farhad Rahmanifar
f.rahmanifar@yahoo.com
1
Amin Tamadon
tamadon@yahoo.com
2
Davood Mehrabani
davood_mehrabani@yahoo.com
3
Shahrokh Zare
zare.shahrokh@gmail.com
4
Sorush Abasi
sorushabbasi@ymail.com
5
Saeideh Keshavarz
saeideh92keshavarz@gmail.com
6
Mehdi Dianatpour
dianatpour@sums.ac.ir
7
Zahra Khodabandeh
zahrabandeh@gmail.com
8
Iman Raze ghian Jahromi
9
Omid Koohi-Hoseinabadi
10
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Laboratory Animal Center, Shiraz University of Medical Sciences, Shiraz, Iran
Objective(s): Bone marrow-derived mesenchymal stem cells (BM-MSCs) potentials make them appropriate for cell therapy including ability of differentiation and release of anti-inflammatory cytokines and growth factors secreta. For treatment of azoospermia to induce proliferation and differentiation of germ cells, MSCs transplantation has been introduced. The aim of the present experimental case-control study was to histomorphometric evaluation of the germinal cells in seminiferous tubules of azoospermic rats before and after BM-MSCs allotransplantation.
Materials and Methods: In the present study, BM-MSCs were isolated from six male rats and confirmed. Their testes also served as intact negative controls. The recipient rats (n=6) were received two doses of 10 mg/kg of busulfan with 21 days interval to induce azoospermia. After cessation of spermatogenesis, the rats were allotransplanted with the BM-MSCs into efferent duct of right testes. Thirty-five days later, the right cell-treated testes were compared to left azoospermic ones.
Results: Histomorphometric analyses showed that the seminiferous tubules treated with BM-MSCs had normal morphology in comparison with azoospermic testes, which were without germinal layer. In most BM-MSCs-treated seminiferous tubules, spermatogenesis was observed.
Conclusion: The allotransplanted BM-MSCs could induce spermatogenesis in seminiferous tubules of azoospermic rats.
https://ijbms.mums.ac.ir/article_7134_1d4d8a85be5bbe23e20e56aaac520719.pdf
Azoospermia
Bone marrow mesenchymal- stem cell
Busulfan
Cell therapy
Rat
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
662
669
10.22038/ijbms.2016.7135
7135
Evaluation of antioxidant and anti-melanogenic activities of different extracts from aerial parts of Nepeta binaludensis Jamzad in murine melanoma B16F10 cells
Zahra Tayarani-Najaran
tayraninz@mums.ac.ir
1
Maryam Akaberi
akaberim911@mums.ac.ir
2
Mohsen Vatani
vatanim@gmail.com
3
Seyed Ahmad Emami
emamia@sums.ac.ir
4
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Objective(s): Nepeta binaludensis Jamzad (Lamiaceae) has been used in folk medicine of Iran to cure various diseases. The plant is an endemic species to the country that has recently been identified in Razavi Khorasan province. To evaluate the antioxidant and anti-melanogenesis of N. binaludensis, in this study the inhibitory activity of different extracts of N. binaludensis in murine melanoma B16F10 cells is investigated.
Materials and Methods: The effects of petroleum ether, dichloromethane, ethyl acetate, and methanol extracts isolated from the plant on melanogenesis in B16 melanoma cells were investigated.To assess the inhibitory effects of this plant on melanogenesis, various assays were used including cytotoxicity, inhibition of mushroom tyrosinase and cellular tyrosinase, determination of melanin content, the effect of extracts on reactive oxygen species and western blot analysis of proteins involved in melanogenesis process.
Results: The content of melanin and the activity of tyrosinase were significantly reduced with different extracts of N. binaludensis in cells. Reactive oxygen species was also significantly decreased following the treatment of cell with the mentioned extracts, while a resazurin assay showed no cytotoxicity. Furthermore, we have found that the plant decreased the amount of tyrosinase and microphthalmia-associated transcription factor proteins, which verify the role of suppression of microphthalmia-associated transcription factor protein in melanogenesis inhibition.
Conclusion: Taken together the data indicate that N. binaludensis has inhibitory activity on melanin synthesis with no cytotoxic effects in B16 melanoma cells. Therefore, it merits future investigations to apply as whitening agent in hyperpigmentation.
https://ijbms.mums.ac.ir/article_7135_57c3d7b1818747d723c8d93f7b30125f.pdf
Lamiaceae
Melanogenesis
Melanoma B16F10
Microphthalmia-associated
Nepeta binaludensis
Tyrosinase
Transcription factor
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
670
675
10.22038/ijbms.2016.7136
7136
Colchicine protects rat skeletal muscle from ischemia/reperfusion injury by suppressing oxidative stress and inflammation
Liangrong Wang
arerong1984@163.com
1
Yuanlu Shan
420512181@qq.com
2
Lei Chen
luckystone@126.com
3
Bi Lin
304811101@qq.com
4
Xiangqing Xiong
flllgend@qq.com
5
Lina Lin
wzlinlina@tom.com
6
Lida Jin
manuscriptjinlida@163.com
7
Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, No. 2, Fuxue Road, Wenzhou 325000, Zhejiang Province, People’s Republic of China.
Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, No. 2, Fuxue Road, Wenzhou 325000, Zhejiang Province, People’s Republic of China.
Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, No. 2, Fuxue Road, Wenzhou 325000, Zhejiang Province, People’s Republic of China.
Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, No. 2, Fuxue Road, Wenzhou 325000, Zhejiang Province, People’s Republic of China.
Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, No. 2, Fuxue Road, Wenzhou 325000, Zhejiang Province, People’s Republic of China.
Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, No. 2, Fuxue Road, Wenzhou 325000, Zhejiang Province, People’s Republic of China.
Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, No. 2, Fuxue Road, Wenzhou 325000, Zhejiang Province, People’s Republic of China.
Objective(s): Neutrophils play an important role in ischemia/reperfusion (IR) induced skeletal muscle injury. Microtubules are required for neutrophil activation in response to various stimuli. This study aimed to investigate the effects of colchicine, a microtubule-disrupting agent, on skeletal muscle IR injury in a rat hindlimb ischemia model. Materials and Methods: Twenty-one Sprague-Dawley rats were randomly allocated into three groups: IR group, colchicine treated-IR (CO) group and sham operation (SM) group. Rats of both the IR and CO groups were subjected to 3 hr of ischemia by clamping the right femoral artery followed by 2 hr of reperfusion. Colchicine (1 mg/kg) was administrated intraperitoneally prior to hindlimb ischemia in the CO group. After 2 hr of reperfusion, we measured superoxide dismutase (SOD) and myeloperoxidase (MPO) activities, and malondialdehyde (MDA), tumor necrosis factor (TNF)-α and interleukin (IL)-1β levels in the muscle samples. Plasma creatinine kinase (CK) and lactate dehydrogenase (LDH) levels were measured. We also evaluated the histological damage score and wet/dry weight (W/D) ratio. Results: The histological damage score, W/D ratio, MPO activity, MDA, TNF-α and IL-1β levels in muscle tissues were significantly increased, SOD activity was decreased, and plasma CK and LDH levels were remarkably elevated in both the IR and CO groups compared to the SM group (P<0.05). Colchicine treatment significantly reduced muscle damage and edema, oxidative stress and levels of the inflammatory parameters in the CO group compared to the IR group (P<0.05). Conclusion: Colchicine attenuates IR-induced skeletal muscle injury in rats.
https://ijbms.mums.ac.ir/article_7136_16ca533be85d425d995db0ec9385efdd.pdf
Colchicine
Inflammation
Muscle
Reperfusion injury
Skeletal
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
676
684
10.22038/ijbms.2016.7137
7137
Effect of interaction between acute administration of morphine and cannabinoid compounds on spontaneous excitatory and inhibitory postsynaptic currents of magnocellular neurons of supraoptic nucleus
Mitra Yousefpour
yousefpour_mi@yahoo.com
1
Nima Naderi
2
Fereshteh Motamedi
motamedi@ams.ac.ir
3
Department of Physiology, Faculty of Medicine, Artesh University of Medical Science, Tehran, Iran
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Neuroscience Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran
Objective(s): Opioids and cannabinoids are two important compounds that have been shown to influence the activity of magnocellular neurons (MCNs) of supraoptic nucleus (SON). The interaction between opioidergic and cannabinoidergic systems in various structures of the brain and spinal cord is now well established, but not in the MCNs of SON.
Materials and methods: In this study, whole cell patch clamp recording of neurons in rat brain slice was used to investigate the effect of acute morphine and cannabinoid administration on spontaneous inhibitory and excitatory spostsynaptic currents (sIPSCs and sEPSCs) in MCNs.
Results: Bath application of morphine produced an increase in sEPSCs frequency and a decrease in sIPSCs frequency. In contrast, bath application of URB597 (fatty acid amide hydrolase (FAAH) inhibitor) produced a decrease in sEPSCs frequency but an increase in sIPSCs frequency. WIN55212-2 (cannabinoid receptor agonist) decreased both sIPSCs and sEPSCs frequencies of MCNs. Co-application of morphine and URB597 attenuated the effect of morphine on MCNs.
Conclusion: Taken together, these data indicated that at the cellular level, pharmacological augmentation of endocannabinoids could attenuate morphine effects on MCNs.
https://ijbms.mums.ac.ir/article_7137_2268e5b714820c94990e983a751756dc.pdf
Cannabinoid
Morphine
sEPSC
sIPSC
Supraoptic nucleus
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2016-06-01
19
6
685
691
10.22038/ijbms.2016.7138
7138
The protective effect of infliximab against carbon tetrachloride-induced acute lung injury
Aysel Kurt
drayselkurt@yahoo.com
1
Levent Tumkaya
2
Suleyman Yuce
3
Hasan Turut
4
Medine Cumhur Cure
5
Ibrahim Sehitoglu
sehitogluibrahim@gmail.com
6
Yildiray Kalkan
ykalkan53@gmail.com
7
Gokhan Pusuroglu
g_pusur@hotmail.com
8
Erkan Cure
9
Recep Tayyip Erdogan University, School of Medicine, Department of Thoracic Surgery, Rize, Turkey
Recep Tayyip Erdogan University, School of Medicine, Department of Histology and Embryology, Rize, Turkey
Department of Internal Medicine, Ordu Kumru State Hospital, Ordu, Turkey
Recep Tayyip Erdogan University, School of Medicine, Department of Thoracic Surgery, Rize, Turkey
Recep Tayyip Erdogan University, School of Medicine, Department of Biochemistry, Rize, Turkey
Recep Tayyip Erdogan University, School of Medicine, Department of Pathology, Rize, Turkey
Recep Tayyip Erdogan University, School of Medicine, Department of Histology and Embryology, Rize, Turkey
Recep Tayyip Erdogan University, School of Medicine, Department of Internal Medicine, Rize, Turkey
Recep Tayyip Erdogan University, School of Medicine, Department of Internal Medicine, Rize, Turkey
Objective(s): Carbon tetrachloride (CCl4) causes pulmonary toxicity. Infliximab (Ib) is a potent inhibitor of tumor necrosis factor-alpha (TNF-α). We aimed to investigate whether Ib has a protective effect on CCl4 induced lung injury.
Materials and Methods:Rats were divided into control, CCl4, and CCl4+Ib groups. A single dose of 2 ml/kg CCI4 was administered to CCI4 group and a single dose of 7 mg/kg Ib was given to CCl4+Ib group 24 hr before applying CCI4.
Results:TNF-α, malondialdehyde (MDA), nitric oxide (NO) and caspase-3 levels of the CCl4 group were markedly higher than both the control and CCl4+Ib groups. The CCI4+Ib group had lower histopathological injury than the CCl4 group.
Conclusion:Ib as a strong TNF-α blocker decreases the production of proinflammatory cytokines, MDA, and oxidative stress leading to a protective effect against CCl4 induced lung tissue injury.
https://ijbms.mums.ac.ir/article_7138_3039f66c559f977304a83ed093dac0e2.pdf
Carbon tetrachloride
Infliximab
Nitric oxide
Pulmonary toxicity
Oxidative stress