eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
581
589
10.22038/ijbms.2019.31879.7666
12723
Signaling pathways involved in chronic myeloid leukemia pathogenesis: the importance of targeting Musashi2-Numb signaling to eradicate leukemia stem cells
Foruzan Moradi
foruzanmoradi69@yahoo.com
1
Sadegh Babashah
sadegh.babashah@gmail.com
2
Majid Sadeghizadeh
sadeghma@modares.ac.ir
3
Arsalan Jalili
jalili.arsalan@yahoo.com
4
Abbas Hajifathali
a.hajifathali@sbmu.ac.ir
5
Elham Roshandel
elham.roshandel@gmail.com
6
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Hematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Hematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Objective(s): Chronic myeloid leukemia (CML) is a myeloid clonal proliferation disease defining by the presence of the Philadelphia chromosome that shows the movement of BCR-ABL1. In this study, the critical role of the Musashi2-Numb axis in determining cell fate and relationship of the axis to important signaling pathways such as Hedgehog and Notch that are essential for self-renewal pathways in CML stem cells will be reviewed meticulously.Materials and Methods: In this review, a PubMed search using the keywords of Leukemia, signaling pathways, Musashi2-Numb was performed, and then we summarized different research works.Results: Although tyrosine kinase inhibitors such as Imatinib significantly kill and remove the cell with BCR-ABL1 translocation, they are unable to target BCR-ABL1 leukemia stem cells. The main problem is stem cells resistance to Imatinib therapy. Therefore, the identification and control of downstream molecules/ signaling route of the BCR-ABL1 that are involved in the survival and self-renewal of leukemia stem cells can be an effective treatment strategy to eliminate leukemia stem cells, which supposed to be cured by Musashi2-Numb signaling pathway.Conclusion: The control of molecules /pathways downstream of the BCR-ABL1 and targeting Musashi2-Numb can be an effective therapeutic strategy for treatment of chronic leukemia stem cells. While Musashi2 is a poor prognostic marker in leukemia, in treatment and strategy, it has significant diagnostic value.
https://ijbms.mums.ac.ir/article_12723_d9b444b5e7550b9883ddcd7802ad293e.pdf
BCR-ABL1
Chronic myeloid leukemia
Cancer stem cells
Signaling pathways
Self-renewal
Targeted therapy
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
590
600
10.22038/ijbms.2019.32963.7873
12771
The role of Iranian medicinal plants in experimental surgical skin wound healing: An integrative review
Amin Derakhshanfar
cbrc@sums.ac.ir
1
Javad Moayedi
javad.moayedi88@gmail.com
2
Ghazal Derakhshanfar
ghazal.derakhshanfar@gmail.com
3
Ali Poostforoosh Fard
alipo58@gmail.com
4
Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Vice Chancellery for Research Affairs, Shiraz University of Medical Sciences, Shiraz, Iran
Objective(s): Wounds are physical injuries that cause a disturbance in the normal skin anatomy and function. Also, it has a severe impact on the cost of health care. Wound healing in human and mammalian species is similar and contains a complex and dynamic process consisting of four phases for restoring skin cellular structures and tissue layers. Today, therapeutic approaches using herbal medicine have been considered. Although the benefits of herbal medicine are vast, some medicinal plants have been shown to have wound healing effects in different experimental studies. Therefore, the current review highlights information about the potency of herbal medicine in the experimental surgical skin wound healing.Materials and Methods: Electronic database such as PubMed, Google Scholar, Scopus, and Medscape were searched for Iranian medicinal plants with healing activity in experimental surgical skin wounds. In this area, some of the most important papers were included.Results: There are numerous Iranian medicinal plants with skin wound healing activity, but clinical application and manufacturing are very low in comparison to the research volume.Conclusion: In normal instances, the human/animal body usually can repair tissue damage precisely and completely; therefore, the utilization of herbs is limited to special conditions or in order to accelerate the healing process.
https://ijbms.mums.ac.ir/article_12771_b58a584b8d3331a8bc184a2c827d6fcf.pdf
Experimental
Iranian medicinal plants
Skin
Surgical
Wound healing
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
601
609
10.22038/ijbms.2019.30138.7266
12562
The effects of crocin on spatial memory impairment induced by hyoscine: Role of NMDA, AMPA, ERK, and CaMKII proteins in rat hippocampus
Maliheh Adabizadeh
adabizadehm2@mums.ac.ir
1
Soghra Mehri
mehris@mums.ac.ir
2
Mahshid Rajabpour
mahshidr@mums.ac.ir
3
Khalil Abnous
abnouskh@mums.ac.ir
4
Marzieh Rashedinia
rashediniam881@mums.ac.ir
5
Hossein Hosseinzadeh
hosseinzadehh@mums.ac.ir
6
Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shiraz University of Medical Sciences Shiraz Iran
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Objective(s): Crocus sativus L. and its active constituent, crocin, have neuroprotective effects. The effects of crocin on memory impairment have been mentioned in studies but the signaling pathways have not been evaluated. Therefore, the aim of this study was to evaluate the effects of crocin on the hyoscine-induced memory impairment in rat. Additionally, the level of NMDA (N-methyl-D-aspartate receptors), AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazole-propionicd acid), ERK (extracellular signal-regulated kinases), CaMKII (calcium (Ca2+)/calmodulin (CaM)-dependent kinaseII) mRNA and proteins were determined in rat hippocampus. Materials and Methods: Crocin (10, 20, and 40 mg/kg), hyoscine (1.5 mg/kg), normal saline and rivastigmine were administered intraperitoneally to male Wistar rats for 5 days. The effects on memory improvement were studied using Morris water maze (MWM) test. Then, the protein levels of NMDA, AMPA, ERK, pERK, CaMKII and p.CaMKII in hippocampus were analized using the Western blot test. Furthermore, the mRNA levels of NMDA, AMPA, ERK and pCaMKII genes were evaluated using real-time quantitative reverse transcription-polymerase chain reaction (qRT- PCR) method. Results: Aadminestration of crocin (20 mg/kg) and rivastigmine significantly improved learning and memory impairment induced by hyoscine. Also, administration of hyoscine reduced protein level of pERK, while treatment with crocin (20 mg/kg) recovered the protein level. No changes were observed in the protein levels and mRNA gene expression of NMDA, AMPA, ERK, CaMKII and pCaMKII following adminestration of hyoscine or crocin. Conclusion: Adminestration of crocin improved memory and learning. The effect of crocin in this model can be mediated by alteration in pERK protein level in rat hippocampus.
https://ijbms.mums.ac.ir/article_12562_230ff1945b345b5941c00abdb0e76ca9.pdf
Crocin
Saffron
Memory
Erk
CaMKII
NMDA
AMPA
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
610
616
10.22038/ijbms.2019.33557.8007
12583
Human leukocyte antigen (HLA)-Cw0303, HLA-Cw04, and HLA-Cw07 polymorphisms are associated with susceptibility of rheumatoid arthritis in Chinese Han patients from Southern China
Chuankun Yang
yangchuankun1985@126.com
1
Chunling Wang
498650591@qq.com
2
Yan Shi
249519007@qq.com
3
Li Li
lilee_immuno@sina.cn
4
Department of Immunology Laboratory, Zhongda Hospital, School of medicine, Southeast University, Nanjing, Jiangsu, 210009, China
Department of Immunology Laboratory, Zhongda Hospital, School of medicine, Southeast University, Nanjing, Jiangsu, 210009, China
Department of Immunology Laboratory, Zhongda Hospital, School of medicine, Southeast University, Nanjing, Jiangsu, 210009, China
Department of Immunology Laboratory, Zhongda Hospital, School of medicine, Southeast University, Nanjing, Jiangsu, 210009, China
Objective(s): This study aimed to investigate the association between human leukocyte antigen Cw (HLA-Cw) polymorphisms and rheumatoid arthritis (RA) in Chinese Han patients in the Jiangsu area (Southern China).Materials and Methods: Polymerase chain reaction-sequence specific primers were used to detect HLA-Cw01–08 of 201 RA patients and 211 healthy individuals from Zhongda Hospital (China). The allele frequency distribution of HLA-Cw and genotypic differences between the two groups were analyzed.Results: The frequency of HLA-Cw0303 in patients with RA was significantly higher than that in controls, while the frequency of HLA-Cw04 was lower than that in controls (P<0.05). The gene frequency of HLA-Cw07 in anti-cyclic citrullinated peptide (anti-CCP)-negative patients was higher than that in controls (P=0.044). The frequency of HLA-Cw04 was decreased in the short duration subgroup and increased in the long duration subgroup (P<0.05). Compared to controls, the frequency of HLA-Cw0303 in patients with RA and morning stiffness was increased (P=0.004), while the frequency of HLA-Cw04 was decreased ( 0.005).Conclusion: These results suggest that HLA-Cw0303 is a susceptibility gene for RA in Chinese Han patients in the Jiangsu area of southern China. The HLA-Cw04 gene may be a protective factor against RA, while HLA-Cw07 might play a protective role in the production of anti-CCP in the long-term course in patients with RA.
https://ijbms.mums.ac.ir/article_12583_15c1fb0b25122fbb5f1435b5914dc471.pdf
Anti-citrullinated protein antibodies
HLA antigens
Gene frequency
Polymorphism
Single nucleotide
Arthritis rheumatoid
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
617
622
10.22038/ijbms.2019.34118.8110
12761
Obestatin inhibits apoptosis and astrogliosis of hippocampal neurons following global cerebral ischemia reperfusion via antioxidant and anti-inflammatory mechanisms
Elahe Mirarab
elahem@gmail.cm
1
Vida Hojati
vidah@yahoo.com
2
Gholamhassan Vaezi
gh.vaezi@yahoo.com
3
Abdolhossein Shiravi
shiraviab@yahoo.com
4
Mehdi Khaksari
khaksari417@yahoo.com
5
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
Objective(s): Obestatin is a newly discovered peptide with antioxidant activities in different animal models. Recent studies have shown that Obestatin inhibits apoptosis following cardiac ischemia/reperfusion injury. Brain ischemia/reperfusion induces irreversible damage especially in the hippocampus area. This study aimed at examining the protective impact of Obestatin on apoptosis, protein expression and reactive astrogliosis level in hippocampal CA1 region of rat following transient global cerebral ischemia.Materials and Methods: Forty-eight male Wistar rats were randomly assigned into 4 groups (sham, ischemia/reperfusion, ischemia/reperfusion+ Obestatin 1, and 5 µg/kg, n=12). Ischemia induced occlusion of both common carotid arteries for 20 min. Obestatin 1 and 5 µg/kg were injected intraperitoneally at the beginning of reperfusion period and 24 and 48 hr after reperfusion. Assessment of the antioxidant enzymes and tumor necrosis factor alpha (TNF-α) was performed by ELISA method. Caspase-3 and glial fibrillary acidic protein (GFAP) proteins expression levels were evaluated by immunohistochemical staining 7 days after ischemia.Results: Based on the result of the current study, lower superoxide dismutase (SOD) and glutathione (GSH) (P<0.05) and higher malondialdehyde (MDA) and TNF-α levels were observed in the ischemia group than those of the sham group (P<0.01). Obestatin treatment could increase both SOD and GSH (P<0.05) and reduce MDA and TNF-α (P<0.05) versus the ischemia group. Moreover, obestatin could significantly decrease caspase-3 and GFAP positive cells in the CA1 region of hippocampus (P<0.01). Conclusion: Obestatin exerts protective effects against ischemia injury by inhibition of astrocytes activation and decreases neuronal cell apoptosis via its antioxidant properties.
https://ijbms.mums.ac.ir/article_12761_92a4a9f133e2473f90bf69a966134743.pdf
Apoptosis
Astrogliosis
Brain ischemia
Hippocampus
Obestatin
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
623
630
10.22038/ijbms.2019.34571.8206
12763
Expression of the receptor of advanced glycation end-products (RAGE) and membranal location in peripheral blood mononuclear cells (PBMC) in obesity and insulin resistance
Elizabeth del Ruelas Cinco
ruelas5_eli@hotmail.com
1
Bertha Ruíz Madrigal
bertharuiz8@yahoo.com.mx
2
José Alfredo Dominguez-Rosales
dominque14@yahoo.com
3
Montserrat Maldonado González
montserratmaldonado@yahoo.com.mx
4
Lucía De la Cruz Color
lucia_color@yahoo.com.mx
5
Sandra Margarita Ramírez Meza
zandhrita_15@hotmail.com
6
José Rodrigo Torres Baranda
rodrigo.torresbaranda@gmail.com
7
Erika Martínez López
erikamtz27@yahoo.com.mx
8
Zamira Helena Hernández Nazará
zamirahelena@yahoo.com.mx
9
Instituto de Investigación en Enfermedades Crónico-Degenerativas, Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
Laboratorio de Investigación en Microbiología, Departamento de Microbiología y Patología, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
Instituto de Investigación en Enfermedades Crónico-Degenerativas, Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
Laboratorio de Investigación en Microbiología, Departamento de Microbiología y Patología, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
Instituto de Investigación en Enfermedades Crónico-Degenerativas, Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
Laboratorio de Investigación en Microbiología, Departamento de Microbiología y Patología, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
Laboratorio de Investigación en Microbiología, Departamento de Microbiología y Patología, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
Programa de Doctorado en Ciencias en Biología Molecular en Medicina. Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
Instituto de Investigación en Enfermedades Crónico-Degenerativas, Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
Objective(s): The present study aimed to evaluate the receptor of advanced glycation end-products (RAGE), NF-kB, NRF2 gene expression, and RAGE cell distribution in peripheral blood mononuclear cells (PBMC) in subjects with obesity and IR compared with healthy subjects.Materials and Methods: The mRNA expression levels of RAGE, NF-kB, NRF2, and GAPDH were determined in PBMC by qPCR in 20 obese (OB), 17 obese with insulin resistance (OB-IR) subjects, and 20 age and sex-matched healthy subjects (HS). RAGE protein expression and its localization were determined by Western Blot and immunocytochemistry (ICC) analysis, total soluble RAGE (sRAGE) and MCP-1 plasma levels by ELISA. Results: RAGE, NF-kB, and NRF2 genes mRNA expression in PBMCs did not show variation between groups. RAGE protein was lower in OB and OB-IR groups; RAGE was located predominantly on the cell-surface in the OB-IR group compared to the HS group (22% vs 9.5%, P<0.001). OB-IR group showed lower sRAGE plasma levels, and correlated negatively with HOMA-IR, ALT parameters (r= -0.374, r= -0.429, respectively), and positively with NFE2L2 mRNA (r= 0.540) PConclusion: In this study, OB-IR subjects did not reflect significant differences in gene expression; however, correlations detected between sRAGE, biochemical parameters, and NRF2, besides the predominant RAGE distribution on the cell membrane in PBMC could be evidence of the early phase of the inflammatory cascade and the subsequent damage in specific tissues in subjects with OB-IR.
https://ijbms.mums.ac.ir/article_12763_55edd33c9cb183a01c9b2cfb8912f745.pdf
AGER protein human
insulin resistance
Obesity
Oxidative stress
Receptor for advanced glycation end products
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
631
636
10.22038/ijbms.2019.32624.7806
12582
Effects of Lactobacillus acidophilus and Bifidobacterium bifidum probiotics on the serum biochemical parameters, and the vitamin D and leptin receptor genes on mice colon cancer
Peyman Ranji
peyman_ranji@yahoo.com
1
Shahram Agah
dr.sh.agah@gmail.com
2
Zahra Heydari
heydarizara@yahoo.com
3
Mohammad Rahmati-Yamchi
rahmatibio@gmail.com
4
Ali Mohammad Alizadeh
aalizadeh@sina.tums.ac.ir
5
Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran
Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran
Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran
Objective(s): The preclinical reports have shown that specific probiotics like Bifidobacterium bifidum (B. bifidum) and Lactobacillus acidophilus (L. acidophilus) can be applied as the biotherapeutic agents in the inhibition or therapy of colorectal cancer via the modification of gut bacteria. In the previous studies, we have assessed the impact of L. acidophilus and B. bifidum probiotics on gut bacteria concentration and also their chemo-protective impact on mice colon cancer. In the following, we assessed the effects of these probiotics on the gene expression of vitamin D receptor (VDR) and the leptin receptor (LPR) and the serum biochemical parameters on mice colon cancer. Materials and Methods: Thirty-six male BALB/c mice were equally shared into 4 groups; (i) health with routine dietary foods without any treatment, (ii) azoxymethane (AOM)-induced mice colon cancer with common dietary foods, (iii) and (iv) AOM-induced mice colon cancer with oral consumption of L. acidophilus and B. bifidum (1×109 cfu/g) for 5 months, respectively. Then, the serum total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), alanine transaminase, alkaline phosphatase, and albumin and also VDR and LPR genes expression were evaluated. Results: Oral consumption of L. acidophilus and B. bifidum probiotics significantly decreased the triglycerides, alkaline phosphatase, LDL, and also the VDR and LPR gene expression in mice colon cancer (P<0.005).Conclusion: L. acidophilus and B. bifidum probiotics with the modification of the biochemical parameters and the expression of the VDR and LPR genes can play a key role in the protection of mouse colon cancer.
https://ijbms.mums.ac.ir/article_12582_e079eecb9e4238dcab3558261d90c7e1.pdf
Colon cancer
Leptin receptor
Mice
Probiotic
Vitamin D Receptor
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
637
642
10.22038/ijbms.2019.34158.8123
12762
Effect of troxerutin on apelin-13, apelin receptors (APJ), and ovarian histological changes in the offspring of high-fat diet fed rats
Keivan Mehri
keivanm20@gmail.com
1
Seyed Mehdi Banan Khojasteh
smbanan@tabrizu.ac.ir
2
Fereshteh Farajdokht
farajdokht@gmail.com
3
Zohreh Zavvari Oskuye
zohre.zavari@gmail.com
4
Hadi Ebrahimi
ehadi2@yahoo.com
5
Rogaye Diba
r1367diba@gmail.com
6
Parvin Bayandor
bayandorbarzegari@gmail.com
7
Maryam Hosseindoost
mhh.hosseindoost@gmail.com
8
Shirin Babri
shirinb46@yahoo.com
9
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Iran
Neurosciences Research Center (NSRC), Tabriz University of Medical Sciences, Tabriz, Iran
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Objective(s): Maternal high-fat diet (HFD) consumption has been linked to metabolic disorders and reproductive dysfunctions in offspring. Troxerutin (TRO) has anti-hyperlipidemic, anti-oxidant, and anti-inflammatory effects. This study examined the effects of TRO on apelin-13, its receptors mRNA and ovarian histological changes in the offspring of HFD fed rats. Materials and Methods: Female Wistar rats were randomly divided into control diet (CD) or HFD groups and received these diets for eight weeks. After mating, dams were assigned into four subgroups: CD, CD + TRO, HFD, and HFD + TRO, and received their respective diets until the end of lactation. Troxerutin (150 mg/kg/day) was gavaged in the CD + TRO and HFD + TRO groups during pregnancy. On the postnatal day (PND) 21 all female offspring were separated and fed CD until PND 90. On PND 90 animals were sacrificed and ovarian tissue samples were collected for further evaluation. Results: Results showed that HFD significantly decreased serum apelin-13 in the female offspring of the HFD dams, which was significantly reversed by TRO. Moreover, real-time polymerase chain reaction (PCR) analysis revealed that TRO treatment significantly decreased the ovarian mRNA expression of the apelin-13 receptor in the troxerutin-received offspring. Furthermore, histological examination revealed that TRO increased the number of atretic follicles in the ovaries of HFD+TRO offspring.Conclusion: Maternal high fat feeding compromises ovarian health including follicular growth and development in the adult offspring and troxerutin treatment improved negative effects of maternal HFD on the apelin-13 level and ovarian development of offspring.
https://ijbms.mums.ac.ir/article_12762_5d2c3f6bc77dc2c601ad73ea1f6f375a.pdf
Apelin-13
APJ receptor
Maternal high-fat diet
Ovarian development
Troxerutin
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
643
649
10.22038/ijbms.2019.33331.7957
12773
Studying humane endpoints in a rat model of mammary carcinogenesis
Ana I Faustino-Rocha
anafaustino.faustino@sapo.pt
1
Mário Ginja
mginja@utd.pt
2
Rita Ferreira
ritaferreira@ua.pt
3
Paula A Oliveira
pamo@utad.pt
4
Faculty of Veterinary Medicine, Lusophone University of Humanities and Technologies, Lisbon, Portugal
Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
Organic Chemistry, Natural Products and Foodstuffs (QOPNA), Mass Spectrometry Center, Department of Chemistry, University of Aveiro, Aveiro, Portugal
Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
Objective(s): The present work intended to clearly define the most adequate humane endpoints in an experimental assay of mammary carcinogenesis in rats. Materials and Methods: Animals were observed twice a day; all parameters were registered once a week and the euthanasia endpoints were established in order to monitor the animal welfare/distress during an experimental assay of chemically-induced mammary carcinogenesis in female rats. Results: Fourteen animals developed at least one mammary tumor with a diameter >35 mm. No animals exhibited alterations in the remaining parameters that implied their early sacrifice. Statistically significant changes were not observed in the quantitative parameters like the hematocrit and urine specific gravity among groups, not being valuable for the assessment of the health status of animals included in an assay of mammary carcinogenesis for 18 weeks. The remaining humane endpoints seemed to be helpful to monitor the animals’ health status.Conclusion: The alteration in only one humane endpoint (mammary tumor dimensions) does not imply the animals’ sacrifice; the endpoints should be evaluated in conjunction, in order to define the most adequate time in which the animals should be sacrificed.
https://ijbms.mums.ac.ir/article_12773_f3cbc32b2a86e7c13e4bf2745efd4dad.pdf
Chemically-induced
Humane endpoints
Mammary cancer
N-methyl-N-nitrosourea
Rat
Welfare
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
650
659
10.22038/ijbms.2019.32576.7797
12720
Pulsed electromagnetic field attenuated PTSD-induced failure of conditioned fear extinction
Mohammad Ali Mohammad Alizadeh
maz.4747@yahoo.com
1
Kataneh Abrari
abrari@du.ac.ir
2
Taghi Lashkar Blouki
lashkar@du.ac.ir
3
mohammad taghi ghorbanian
ghorbanian@du.ac.ir
4
Majid Jadidi
jadidim@semums.ac.ir
5
School of Biology, Damghan University, Damghan, Semnan, Iran
School of Biology, Damghan University, Damghan, Semnan, Iran
School of Biology, Damghan University, Damghan, Semnan, Iran
School of Biology, Damghan University, Damghan, Semnan, Iran
Department of Medical Physics, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
Objective(s): This study aimed to determine whether exposure to pulsed electromagnetic field (PEMF) can impair behavioral failure as induced by PTSD, and also its possible effects on hippocampal neurogenesis. PEMF was used as a non-invasive therapeutic tool in psychiatry.Materials and Methods: Male rats were divided into Control-Sham exposed, Control-PEMF, PTSD-Sham exposed, and PTSD-PEMF groups. PTSD rats were conducted by the single prolonged stress procedures and then conditioned by the contextual fear conditioning apparatus. Control rats were only conditioned. Experimental rats were submitted to daily PEMF (7 mT, 30 Hz for 16 min/day, 14 days). Sham-exposed groups were submitted to the turned off PEMF apparatus. Fear extinction, sensitized fear and anxiety, cell density in the hippocampus, and proliferation and survival rate of BrdU-labeled cells were evaluated. Results: Freezing of PTSD-PEMF rats was significantly lower than PTSD-Sham exposed. In the PTSD-PEMF, center and total crossing in open field, also the percentage of open arms entry and time in the elevated plus maze, significantly increased as compared with PTSD-Sham exposed (P<0.001). Numbers of CA1, CA3, and DG cells in PTSD-PEMF and Control-Sham exposed groups were significantly more than PTSD-Sham exposed (P<0.001). There were more BrdU-positive cells in the DG of the PTSD-PEMF as compared with the PTSD-Sham exposed. Qualitative observations showed an increased number of surviving BrdU-positive cells in the PTSD-PEMF as compared with PTSD-Sham exposed.Conclusion: Using 14-day PEM attenuates the PTSD-induced failure of conditioned fear extinction and exaggerated sensitized fear, and this might be related to the neuroprotective effects of magnetic fields on the hippocampus.
https://ijbms.mums.ac.ir/article_12720_3aaf1f52e11e5679796f7ad483ccfadb.pdf
Classical conditioning
Electromagnetic Fields
Hippocampus
Neurogenesis
Post-Traumatic Stress
disorder
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
660
668
10.22038/ijbms.2019.30840.7440
12770
Iron chelation effect of curcumin and baicalein on aplastic anemia mouse model with iron overload
Dijiong Wu
wdj850@163.com
1
Xiaowen Wen
xiaowenax@163.com
2
Linglong Xu
daniongniong@163.com
3
Wenbin Liu
liouwenbin6666@163.com
4
Huijin Hu
huhuijin12@sina.com
5
Baodong Ye
13588453501@163.com
6
Yuhong Zhou
zyhblood@163.com
7
Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
Department of Internal Medicine, Central Hospital of Jinhua Affiliated to Zhejiang University, Jinhua, Zhejiang, China
Department of Hematology, Taizhou Central Hospital, Taizhou, Zhejiang, China
Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
Objective(s): The current work aimed to assess whether curcumin and baicalein can chelate iron in aplastic anemia (AA) complicated with iron overload, exploring the potential mechanisms.Materials and Methods: A mouse model of AA with iron overload complication was firstly established. Low and high-dose curcumin or baicalein treatment groups were set up, as well as the deferoxamine positive control, normal and model groups (n=8). Hemogram and bone marrow mononuclear cell detection were performed, and TUNEL and immunohistochemical staining were used to evaluate hematopoiesis and apoptosis in the marrow. ELISA, Western blot, and qRT-PCR were employed to assess serum iron (SI), serum ferritin (SF), bone morphogenetic protein 6 (BMP-6), SMAD family member4 (SMAD4) and transferrin receptor 2 (TfR2) amounts. Results: Both curcumin and baicalein improved white blood cell (increase of 0.28-0.64×109/l in high-dose groups) and hemoglobin (increase of around 10 g/l) amounts significantly, which may related to decreased apoptosis (nearly 30%-50% of that in the model group) in the bone marrow, while their effects on platelet recovery were limited and inferior to that of deferoxamine (DFO). Both test compounds up-regulated hepcidin and its regulators (BMP-6, SMAD, and TfR2) at the protein and mRNA levels; high dosage treatment may be beneficial, being better than DFO administration in lessening iron deposition in the bone marrow.Conclusion: Curcumin and baicalein protect hematopoiesis from immune and iron overload-induced apoptosis, exerting iron chelation effects in vivo.
https://ijbms.mums.ac.ir/article_12770_1e0d52f05545e5352aca6eac7872d4e9.pdf
Anemia
Animal
Aplastic
Baicalein
Curcumin
Deferoxamine
Iron overload
Mice
Models
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
669
675
10.22038/ijbms.2019.30799.7427
12760
Study of the immunogenicity of outer membrane protein A (ompA) gene from Acinetobacter baumannii as DNA vaccine candidate in vivo
Hossein Ansari
hosseinansari62@gmail.com
1
Maryam Tahmasebi Birgani
maryam_tahmaseby@yahoo.com
2
Mahdi Bijanzadeh
mbijanz@yahoo.com
3
Abbas Doosti
abbasdoosti@yahoo.com
4
Mohammad Kargar
microkargar@gmail.com
5
Department of Genetics, Marvdasht branch, Islamic Azad University, Marvdasht, Iran
Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
Objective(s): Acinetobacter baumannii is one the most dangerous opportunistic pathogens in hospitalized infections. This bacterium is resistant to 90% of commercial antibiotics. Therefore, developing new strategies to cure A. baumannii-infections is urgent. The DNA vaccines new approach in which the immunogen can be directly expressed inside the target cells through cloning of immunogen into an expression vector. The outer membrane protein A(OmpA) is one the critical factors in pathogenicity of A. baumannii which has been repeatedly described as a powerful immunogen to trigger the immune responses. As the pure form of the OmpA is insoluble, vaccine delivery is very hard. Materials and Methods: We previously cloned the ompA gene from A. baumannii into the eukaryotic expression vector pBudCE4.1 and observed that the OmpA protein has been considerably expressed in eukaryotic cell model. In current study, the immunogenic potential of pBudCE4.1-ompA has been evaluated in mice model of experimental. The serum levels of IgM, IgG, IL-2, IL-4, IL-12 and INF-γ were measured by enzyme-linked immunosorbent assay (ELISA) after immunization with ompA-vaccine. The protective efficiency of the designed-DNA vaccine was evaluated following intranasal administration of mice with toxic dose of A. baumannii.Results: Obtained data showed the elevated levels of IgM, IgG, IL-2, IL-4, IL-12 and INF-γ in serum following the vaccine administration and mice who immunized with recombinant vector were survived more than control group.Conclusion: These findings indicate ompA-DNA vaccine is potent to trigger humoral and cellular immunity responses although further experiments are needed.
https://ijbms.mums.ac.ir/article_12760_d92356a136a673d0905f1125e2e2bc62.pdf
Acinetobacter baumannii
OmpA Outer membrane protein
ompA gene
DNA vaccine
Immunomodulation
In vivo
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
676
682
10.22038/ijbms.2019.34529.8190
12774
Mirtazapine may show anti-hyperglycemic effect by decreasing GLUT2 through leptin and galanin expressions in the liver of type 1 diabetic rats
Ezgi Bektur
ezgi.bektur@gmail.com
1
Erhan ŞAHİN
erhansahinn@gmail.com
2
Cengiz Baycu
cbaycu@gmail.com
3
Eskisehir Osmangazi University, Faculty of Medicine, Department of Histology and Embryology, Eskisehir, Turkey
Eskisehir Osmangazi University, Faculty of Medicine, Department of Histology and Embryology, Eskisehir, Turkey
Okan University, Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey
Objective(s): The aim of this study was to explore the molecular mechanism of mirtazapine with respect to energy metabolism in Streptozotocin-induced diabetic liver of rats by immunohistochemistry and Western blot. Materials and Methods: Twenty-one male Sprague-Dawley rats were assigned into 3 groups including control, type 1 diabetes mellitus (T1DM) group (55 mg/kg Streptozocin, IP) and T1DM+mirtazapine (20 mg/kg,PO) group. At the end of the experiment, blood glucose levels were measured and liver tissues were stained by Periodic acid–Schiff. Moreover, leptin and glucose transporter 2 (GLUT2) proteins were analyzed by western blot and immunohistochemistry; however, galanin were analyzed only by immunohistochemistry.Results: At the end of the study, in diabetes group, blood glucose level, GLUT2 and galanin expressions increased, while leptin expression decreased when compared to control group. Mirtazapine treatment restored the decreased leptin expression, and the increased blood glucose level and galanine expression to the level of the control group. It also decreased the GLUT2 expression even below the control group. Conclusion: We concluded that mirtazapine may show its anti-hyperglycemic effect by decreasing GLUT2 through altering the leptin and galanin expression in the liver of type 1 diabetic rats. Mirtazapine can be used as an antidepressant for T1DM patients and as a drug to reduce blood glucose level in T1DM.
https://ijbms.mums.ac.ir/article_12774_5841c336a7e3c87495944bbdca75cd1e.pdf
Galanin
GLUT2
Leptin
Liver
Mirtazapine
Type 1 Diabetes Mellitus
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
683
689
10.22038/ijbms.2019.32670.7812
12795
The protective effect of bone marrow-derived mesenchymal stem cells in liver ischemia/reperfusion injury via down-regulation of miR-370
Mohamad Ali Zare
zarema@sums.ac.ir
1
Abdolhossein Zare
zareab@sums.ac.ir
2
Negar Azarpira
negarazarpira@yahoo.com
3
Sara Pakbaz
pakbazs@sums.ac.ir
4
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Pathology, University of Toronto, Toronto, Canada
Objective(s): Liver transplantation is the most important therapy for end-stage liver disease and ischemia reperfusion (I/R) injury is indeed a risk factor for hepatic failure after grafting. The role of miRNAs in I/R is not completely understood. The aim of this study was to investigate the potential protective role of the mesenchymal stem cells (MSCs) and ischemic preconditioning on miR-370 expression and tissue injury in hepatic I/R injury. Materials and Methods: In this study, 24 BALB/c mice were divided into 4 groups, including sham, I/R, I/R mouse that received MSCs (I/R+MSC) and ischemia preconditioning (IPC) The expression levels of hepatic miR-370, Bcl2 and BAX in male BALB/c mice in different groups including hepatic I/R, hepatic I/R received MSCs, and hepatic I/R with IPC were assessed by quantitative real-time PCR. The effect of miR-370 on hepatic I/R was investigated by serum liver enzyme analysis and histological examination. Results: The expression of miR-370 was significantly up-regulated in the mice subjected to hepatic I/R injury as compared with the sham operated mice. Injection of MSCs led to the down-regulation of the serum liver enzymes, expression of miR-370 and BAX, up-regulation of Bcl2 as well as the improvement of hepatic histological damage. IPC led to similar results, but the difference was not significant. Conclusion: Our data suggest that miR-370 affected the Blc2/BAX pathway in hepatic I/R injury, and down- regulation of miR-370 by BM-MSCs efficiently attenuated the liver damage.
https://ijbms.mums.ac.ir/article_12795_9e4dd2373d1010ca3445e66e19ecd35a.pdf
Apoptosis
Bcl2
Bax
Ischemia reperfusion injury
Mesenchymal stem cells
microRNA 370
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
690
694
10.22038/ijbms.2019.36054.8584
12799
Vitamin D suppresses cellular pathways of diabetes complication in liver
Hoda Derakhshanian
derakhshanian@gmail.com
1
Mahmoud Djalali
mjalali87@yahoo.com
2
Mohammad Hassan Javanbakht
mhjavan2001@yahoo.com
3
Ehsan Alvandi
ehsanalvandi@gmail.com
4
Mohhamd Reza Eshraghian
eshraghianmr@yahoo.com
5
Abbas Mirshafiey
mirshafiey@tums.ac.ir
6
Hoda Nadimi
hodanadimi@gmail.com
7
Samane Jahanabadi
sjahanabadi@yahoo.com
8
Mahnaz Zarei
mahnazzareei@yahoo.com
9
Abolghassem Djazayery
jazaiers@tums.ac.ir
10
Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
Department of Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
Department of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
Objective(s): The aim of this study was to investigate the effect of vitamin D on glucose metabolism, as well as the expression of five key genes involved in the development of diabetes complications in liver tissue of diabetic rats. Materials and Methods: Twenty-four male Sprague–Dawley rats were randomly divided into three groups (8 rats in each group). The first group served as control and the other two groups received an intraperitoneal injection of 45 mg/kg streptozotocin to develop diabetes. Groups were treated for four weeks either with placebo or vitamin D (two injections of 20000 IU/kg). Thereafter, serum levels of glucose, insulin and HbA1c were assessed. Liver tissue was examined for the level of advanced glycation end products (AGEs) and the gene expression of AGE cellular receptor (AGER), glyoxalase-1 (GLO-1), aldose reductase (AR), O-linked N-acetylglucosamine transferase (OGT) and glutamine/ fructose-6-phosphate aminotransferase (GFAT). Results: Vitamin D injection resulted in a significant increase in plasma level of 25-hydroxycholecalciferol, which could improve hyperglycemia about 11% compared to placebo-receiving diabetic rats (P=0.005). Insulin level increased as a result of vitamin D treatment compared to control (3.31±0.65 vs. 2.15±0.79; P= 0.01). Serum HbA1c and liver AGE concentrations had a slight but insignificant reduction following vitamin D intake. Moreover, a significant decline was observed in gene expression of AGER and OGT in liver tissue (P=0.04 and PConclusion: Vitamin D might contribute in ameliorating diabetes complications not only by improving blood glucose and insulin levels, but also by suppressing AGER and OGT gene expression in the liver.
https://ijbms.mums.ac.ir/article_12799_ee28c6ec62aa21814df38f756072f6a7.pdf
Advanced Glycation End Products
Cholecalciferol
Diabetes Complications
Hexosamine pathway
Vitamin D
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
695
702
10.22038/ijbms.2019.33025.7890
12772
Catechol-o-methyltransferase inhibitor tolcapone improves learning and memory in naïve but not in haloperidol challenged rats
Anita Mihaylova
animihailova@gmail.com
1
Hristina Zlatanova
h.zlatanova@gmail.com
2
Nina Doncheva
ninanina1972@abv.bg
3
Delian Delev
delevg@gmail.com
4
Ilia Kostadinov
ilia_197575@abv.bg
5
Department of Pharmacology and Drug Toxicology, Faculty of Pharmacy, Medical University Plovdiv, 15A Vassil Aprilov Blvd., Plovdiv 4002, Bulgaria
Department of Pharmacology and Clinical Pharmacology, Faculty of Medicine, Medical University Plovdiv, 15A Vassil Aprilov Blvd., Plovdiv 4002, Bulgaria
Department of Pharmacology and Drug Toxicology, Faculty of Pharmacy, Medical University Plovdiv, 15A Vassil Aprilov Blvd., Plovdiv 4002, Bulgaria
Department of Pharmacology and Clinical Pharmacology, Faculty of Medicine, Medical University Plovdiv, 15A Vassil Aprilov Blvd., Plovdiv 4002, Bulgaria
Department of Pharmacology and Clinical Pharmacology, Faculty of Medicine, Medical University Plovdiv, 15A Vassil Aprilov Blvd., Plovdiv 4002, Bulgaria
Objective(s): Dopamine plays an important role in cognitive functions. Inhibition of the dopamine-degrading enzyme catechol-O-methyltransferase (COMT) may have beneficial effects. Our aim was to assess the effect of COMT inhibitor tolcapone (TCP) on learning and memory in naïve and haloperidol-challenged rats.Materials and Methods: Male Wistar rats were divided into 9 groups (n=8): naïve-saline, tolcapone 5; 15 and 30 mg/kg BW; haloperidol (HP) challenged-saline, haloperidol, haloperidol+tolcapone 5; 15 and 30 mg/kg BW. Two-way active avoidance test (TWAA), elevated T-maze, and activity cage were performed. Observed parameters were: number of conditioned responses (CR) and unconditioned responses (UCR), working memory index, and vertical and horizontal movements. Results: Naïve rats with 30 mg/kg BW TCP had a significantly increased number of CR and UCR during the long-term memory test. The animals with 5 mg/kg BW TCP significantly increased the number of UCR during the two retention tests. In haloperidol-challenged rats, the three experimental groups decreased the number of CR and UCR during the learning session and the two memory tests, compared to the saline group. There was no significant difference between the HP-challenged rats treated with TCP and the haloperidol control group. All experimental naïve groups had significantly increased working memory index whereas none of the HP-challenged groups showed significant increase in this parameter. Conclusion: Our results demonstrate that in naïve rats tolcapone improves memory in the hippocampal-dependent TWAA task and spatial working memory in T-maze.
https://ijbms.mums.ac.ir/article_12772_6f563c6beb866d7ca985ae3cf4d7ddbc.pdf
COMT
Dopamine
Hippocampus
Prefrontal cortex
Spatial Memory
Tolcapone
Working memory
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2019-06-01
22
6
703
709
10.22038/ijbms.2019.33223.7936
12798
Long-term oral intake of Panax ginseng improves hypomagnesemia, hyperlactatemia, base deficit, and metabolic acidosis in an alloxan-induced rabbit model
Gareeballah Osman Adam
gorba000@gmail.com
1
Gi-Beum Kim
kgb70@jbnu.ac.kr
2
Sei-Jin Lee
lsj@kbsi.re.kr
3
Hee-Ryung Lee
20155485@jbnu.ac.kr
4
Shang-Jin Kim
abbasj@jbnu.ac.kr
5
Hyng-Sub Kang
kang-hs@jbnu.ac.kr
6
Jin-Shang Kim
kimjs@jbnu.ac.kr
7
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596 Republic of Korea
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596 Republic of Korea
Korea Basic Science Institute Jeonju Centre, Deokjin gu, Jeonju-si, Jeollabuk do 54896, Republic of Korea
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596 Republic of Korea
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596 Republic of Korea
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596 Republic of Korea
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596 Republic of Korea
Objective(s): Panax ginseng (PG) widely used for its various pharmacological activities, including effects on diabetes and its complications. This study aims to investigate the effect of PG on mortality-related hypomagnesemia, hyperlactatemia, metabolic acidosis, and other diabetes-induced abnormalities. Materials and Methods: Type 1 diabetes was induced by IV injection of alloxan monohydrate 110 mg/kg into New Zealand white rabbits weighing 2-2.5 kg. PG was supplied in drinking water for 20 weeks. The effects of the PG treatment on diabetes were evaluated through hematological and biochemical analysis including ELISA assays for insulin and glycated haemoglobin A1c (HBA1c) before and after PG extract was supplied. Results: The serum glucose, insulin, and HBA1c levels were significantly improved after the PG treatment compared to those found before PG treatment. In addition, Mg2+, lactate, and base deficit, and acidosis was significantly enhanced in treated rabbits. Moreover, PG showed hepato- and renoprotective effect. Likewise, electrolytes, lipid and protein profile were improved.Conclusion: The biochemical and hematological analysis data demonstrate that the PG is effective to alleviate the diabetes serious signs.
https://ijbms.mums.ac.ir/article_12798_90c8e2fe8a31691beeb652841da5db4f.pdf
Acidosis
Glycated hemoglobin A1c
Hyperlactatemia
Hypomagnesemia
Panax ginseng
Type 1 diabetes