eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2014-01-01
17
1
1
8
10.22038/ijbms.2014.2148
2148
Evaluation of immune response after moderate and overtraining exercise in wistar rat
Zahra Gholamnezhad
1
Mohammad Hossein Boskabady
boskabadymh@mums.ac.ir
2
Mahmoud Hossein
3
Mojtaba Sankian
4
Abolfazl Khajavi Rad
5
Neurogenic Inflammation Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Neurogenic Inflammation Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Neurocognitive Research Centre, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Neurogenic Inflammation Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Objective(s): The effect of prolonged overtraining on cytokine kinetics was compared with moderate exercise in the present study. Materials and Methods: Male Wistar rats were randomly divided into control sedentary (C), moderate trained (MT), (V=20 m/min, 30 min/day for 6 days a week, 8 weeks), overtrained (OT) (V=25 m/min, 60min/day for 6 days a week, 11 weeks) and recovered overtrained (OR) (OT plus 2 weeks recovery) groups, (n=6 for each group). Immediately, 24 hr and 2 weeks (in OR) after last bout of exercise blood samples were obtained. The plasma concentrations of TNFα, IL-6, IL-10, IL-4 and IFN were measured by ELISA method. Results: Immediately after last bout of exercise the following findings were observed; IL-6, IL-10 and TNFαconcentrations increased in OT and OR groups compared with control (P<0.05–P<0.001). Serum level of IL-4 decreased (P<0.01) but IFN increased (P<0.05) in MT group vs. control. In addition, circulatory levels of TNFα IL-6, IL-10 and IL-4 were higher but the IFN concentrations were lower in OT and OR groups than MT group (P<0.05-P<0.01). The IFN-γIL4 ratio was significantly increased in MT (P<0.01) while it decreased in OT group. There were not statistical differences in TNFα IL-6, and IFN levels between different time intervals after exercise in MT, OT and OR groups. Conclusion: These data confirm a positive effect of moderate exercise on immune function and a decrease in susceptibility to viral infection by inducing Th1 cytokine profile shift. However, prolonged and overtraining exercise causes numerous changes in immunity that possibly reflects physiological stress and immune suppression.
https://ijbms.mums.ac.ir/article_2148_9ff0a6a6b98ee77c5507864de248c221.pdf
Immune system
Moderate exercise
Overtraining exercise
Rat
Th1
Th2
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2014-01-01
17
1
9
13
10.22038/ijbms.2014.2149
2149
Effects of chronic crocin treatment on desoxycorticosterone acetate (doca)-salt hypertensive rats
Mohsen Imenshahidi
1
Bibi Marjan Razavi
2
Ayyoob Faal
3
Ali Gholampoor
4
Seyed Mehran Mousavi
5
Hossein Hosseinzadeh
6
Pharmaceutical Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Targeted Drug Delivery Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Pharmaceutical Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Objective(s): In this study, the effects of chronic administration of crocin, an active constituent of saffron, on blood pressures of normotensive and desoxycorticosterone acetate (DOCA) - salt induced hypertensive rats, were investigated. Materials and Methods: Five week administration of three doses of crocin (50, 100 and 200 mg/kg/day) and spironolactone (50 mg/kg/day) in different groups of normotensive and hypertensive rats (at the end of 4 weeks treatment by DOCA-salt) was carried out and their effects on mean systolic blood pressure (MSBP) and heart rate (HR) were evaluated using tail cuff method. The duration of effect of crocin on SBP, was also evaluated. Results: Our results indicated that chronic administration of crocin could reduce the MSBP in DOCA salt treated rats in a dose dependent manner. Crocin did not decrease the MSBP in normotensive rats. The data also showed that antihypertensive effects of crocin did not persist. Conclusion: It is concluded that crocin possesses antihypertensive and normalizing effect on BP in chronic administration.
https://ijbms.mums.ac.ir/article_2149_66299da03eb62263ca88c63e04b9951c.pdf
Crocus sativus
Saffron
Systolic blood pressure
Tail cuff
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2014-01-01
17
1
14
20
10.22038/ijbms.2014.2150
2150
Chitosan and polyvinyl alcohol composite films containing nitrofurazone: preparation and evaluation
Maryam Kouchak
koochekm@yahoo.com
1
Abdolghani Ameri
ameri_ab@yahoo.com
2
Basireh Naseri
naseri_f84@yahoo.com
3
Sara Kargar Boldaji
4
Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Department of Pharmaceutics, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Microbiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4 Department of Food and Drug Control, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
1 Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Objective(s): The aim of this study was to insert nitrofurazone in a chitosan membrane to be used as a wound dressing. Materials and Methods: Several blend films using chitosan (Cs) and polyvinyl alcohol (PVA), containing nitrofurazone were prepared by means of casting/solvent evaporating technique. Different characteristics such as mechanical properties, water vapor transmission rate (WVTR), oxygen permeability (OP), swelling ability (SW), differential scanning calorimetric (DSC), drug release profiles and antibacterial activity of the films were investigated. Results: The results showed that nitrofurazone decreased tensile strength, OP and SW of Cs films, while increased WVTR. Addition of PVA at any concentration improved mechanical properties, reduced WVTR, and increased OP and SW of nitrofurazone-loaded Cs films. The latter films showed higher activity against Pseudomonas aeruginosa than drug-free chitosan films. Conclusion: The presence of PVA improves many properties of Cs-nitrofurazone films and makes them more desirable as dressing material for burn wounds. Although nitrofurazone alone is ineffective against P. aeruginosa, it is able to increase antibacterial effect of chitosan in composite films
https://ijbms.mums.ac.ir/article_2150_f759f0ffe02ab4eaae5f7aa929f8b655.pdf
Antibacterial
Chitosan
Nitrofurazone
Polyvinyl alcohol
Wound dressing
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2014-01-01
17
1
21
26
10.22038/ijbms.2014.2151
2151
Phenotypic and genotypic characteristics of tetracycline resistant Acinetobacter baumannii isolates from nosocomial infections at Tehran hospitals
Mohammad Hossein Maleki
1
Zamberi Sekawi
2
Setareh Soroush
3
Farid Azizi-Jalilian
4
Khairollah Asadollahi
5
Sattar Mohammadi
6
Mohammad Emaneini
7
Morovat Taherikalani
taherikalani@gmail.com
8
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, University of Putra, Malaysia
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.Department of Microbiology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
2 Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, University of Putra, Malaysia
Psychosocial Injuries Research Center, Ilam University of Medical Sciences, Ilam, Ira
Department of Microbiology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
2 Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, University of Putra, Malaysia
Objective(s):To date, the most important genes responsible for tetracycline resistance among Acinetobacter baumannii isolates have been identified as tet A and tet B. This study was carried out to determine the rate of resistance to tetracycline and related antibiotics, and mechanisms of resistance. Materials and Methods: During the years 2010 and 2011, a total of 100 A. baumannii isolates were recovered from patients in different hospitals of Tehran, Iran. Antimicrobial susceptibility to tetracycline, minocycline, doxicycline and tigecycline was evaluated by E-test. Polymerase chain reaction (PCR) of the tet A and tet B genes was performed using specific primers, after which the isolates were subjected to Repetitive Extragenic Palindromic-PCR (PCR) to identify the major genotypes. Results: Of all isolates, 89% were resistant to tetracycline (MIC50 = 32 μg/ml, MIC90 = 512 μg/ml). Minocycline with the resistant rate of 35% (MIC50 = 16 μg/ml, MIC90 =32 μg/ml) and doxicycline with the resistant rate of 25% (MIC50 = 16 μg/ml, MIC90= 32 μg/ml) have a good activity against A. baumannii isolates. All isolates were sensitive to tigecycline. Frequencies of tet B and tet A genes and coexistence of tet A and tet B among the isolates resistant to tetracycline, were 87.6%, 2.2% and 1.1%, respectively. Distribution of REP-types among A. baumannii isolates was types A (40%), B (30%), C (10%), D (5%) and E (5%). Conclusion: It seems that tet A and tet B genes play an important role in the induction of resistance towards tetracyclines used in this study. It is suggested that further studies focus on other antimicrobial drugs and combinations in order to achieve a successful therapy against multi drug resistance (MDR) A. baumannii strains in Iran.
https://ijbms.mums.ac.ir/article_2151_ae21211acd382b50dfb5efd3580c3268.pdf
Acinetobacter baumannii
REP-PCR
Tet A
Tet B
Tetracycline resistance
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2014-01-01
17
1
27
33
10.22038/ijbms.2014.2152
2152
Comparison of the liver function and hepatic specific genes expression in cultured mesenchymal stem cells and hepatocytes
Zahra Nikoozad
1
Mohammad Taghi Ghorbanian
2
Arezou Rezaei
3
School of Biology, Damghan University, Damghan, Iran
School of Biology, Damghan University, Damghan, Iran
2 Institute of Biological Sciences, Damghan University, Damghan, Iran
School of Biology, Damghan University, Damghan, Iran
2 Institute of Biological Sciences, Damghan University, Damghan, Iran
Objective(s): Stem cell therapy is believed to be as a promising treatment strategy for tissue repair and regeneration. The plasticity specification of the adult stem cells, such as MSCs, has enabled that these cells to be used in the treatment of a broad spectrum of diseases like liver disorders. In this study, the production of urea and Albumin (Alb), glycogen storage, and expression of some liver genes including α -fetoprotein (AFP), Alb, cytokeratin18 (CK18) and cytokeratin19 (CK19) was compared between mesenchymal stem cells (MSCs) and isolated rat hepatocytes. Materials and Methods: The MSCs were isolated from rat femurs and tibias and cultured in α -MEM, DMEM and RPMI mediums supplemented with serum. Hepatocytes were isolated from Rat livers and cultured in DMEM with serum. The expression of AFP, Alb, CK18, and CK19 genes was evaluated using the reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, the synthesis of albumin and urea of the cells was measured Results: In vitro conditions, MSCs and hepatocytes exhibited the characteristic functions of the liver such as capacity to synthesize Alb, urea, the storage of glycogen. In this study, the expression of some liver genes such as AFP, Alb, CK18 and CK19 at mRNA levels was also shown.
https://ijbms.mums.ac.ir/article_2152_eb1449e659d9a4bdbb1dbb01f4a99be1.pdf
Albumin
Glycogen
Hepatocytes
Liver genes
Mesenchymal stem cells
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2014-01-01
17
1
34
40
10.22038/ijbms.2014.2153
2153
Stress during first pregnancy increases seizure threshold in adult male offspring
Peyman Pajand
1
Mahmoud Elahdadi Salmani
elahdadi@du.ac.ir
2
Hooman Shajiee
3
Hasan Abiri
4
Iran Goudarzi
irangoudarzi@du.ac.ir
5
Kataneh Abrari
abrari@du.ac.ir
6
Azad Islamic University-Damghan Branch, Damghan, Iran
School of Biology, Damghan University, Damghan, Iran
3 Institute of Biological Sciences, Damghan University, Damghan, Iran
Azad Islamic University-Damghan Branch, Damghan, Iran
Azad Islamic University-Damghan Branch, Damghan, Iran
School of Biology, Damghan University, Damghan, Iran
3 Institute of Biological Sciences, Damghan University, Damghan, Iran
School of Biology, Damghan University, Damghan, Iran
3 Institute of Biological Sciences, Damghan University, Damghan, Iran
Objective(s): Stress induces many homeostatic aberrations which are followed by lifelong allostatic responses. Epilepsy is developed or influenced by different environmental factors, i.e. prenatal stress which makes many contradictory developmental changes in seizure threshold and intensity. We investigated the potential seizure response of the rat offspring to prenatal stress; the stress which was applied to their mothers. Materials and Methods: Nine day heterogeneous sequential stress (HSS) model was used before and during the first and before the second pregnancy. The kindling was induced using 13 IP injections of pentylenetetrazol (PTZ) every 48 hr to adult male Wistar rat's offspring. Results: The results of the present study demonstrated that, before pregnancy stress decreased the rate of kindling (P<0.05) in the offspring, while stress which was applied during pregnancy completely prevented kindling (P <0.001). Further, their convulsive latency was increased and tonic clonic seizure duration was decreased. In contrast, previous pregnancy and between pregnancies stress could not change kindling process. Although maternal separation stress did not change kindling development, it could increase convulsive intensities by elongating the duration of seizures (P<0.05) and reducing convulsion latency (P <0.05). Conclusion: It is concluded that stress detrimental effects could be prevented by stress which was applied around first pregnancy; however this beneficial effect is weakened by before second pregnancy stress.
https://ijbms.mums.ac.ir/article_2153_99b650bbf1d71ac9441097d195901c1c.pdf
Kindling
Pentylenetetrazol
Seizure
pregnancy
stress
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2014-01-01
17
1
41
48
10.22038/ijbms.2014.2154
2154
The effects of losartan on memory performance and leptin resistance induced by obesity and high-fat diet in adult male rats
Seyydeh Gohar Sharieh Hosseini
1
Saeed Khatamsaz
2
Mehrdad Shariati
3
Islamic Azad University, Kazerun Branch, Department of Physiology, Kazerun, Iran
Islamic Azad University, Zarghan Branch, Iran
Islamic Azad University, Kazerun Branch, Department of Physiology, Kazerun, Iran
Objective(s): Leptin is a hormone secreted by adipose tissue and is involved not only in the regulation of feeding and energy expenditure, but also its role in memory enhancement has been demonstrated as well. The partial transfer of leptin across the blood-brain barrier in obese individuals causes leptin resistance and prevents leptin reaching brain. On the other hand, studies have shown that angiotensin antagonists such as losartan can improve memory and learning abilities. The aim of this study was to evaluate the effects of losartan on improving memory and leptin resistance induced by high fat diet in obese rats. Materials and Methods: 40 Wistar male rats were divided in 4 groups: control (C), losartan (LOS), high-fat diet (HFD) and high-fat diet and losartan (HFD and LOS). The spatial memory performances of the rats were assessed in the Morris water maze after 2 months of treatment. Then they were weighed and serum levels of leptin and triglyceride were measured. Results: In spite of receiving high-fat diet, no significant differences in body weight were observed in the (HFD & LOS) group. In the Morris water maze trial, the (LOS) and (HFD & LOS) groups also showed a significant reduction (P <0.05) in latency and path length. In addition, a significant decrease (P <0.05) in serum levels of leptin and no significant difference in serum levels of triglyceride was observed in the (HFD & LOS) group. Conclusion: Losartan can improve leptin resistance induced by obesity and high fat diet. At the same time, it modulates body weight and enhances learning and memory.
https://ijbms.mums.ac.ir/article_2154_d1e3cae1492125570b922476a2df3202.pdf
Leptin resistance
Losartan
Learning
Memory performance
Morris Water Maze
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2014-01-01
17
1
49
54
10.22038/ijbms.2014.2155
2155
Assessment of HTLV-I proviral load, HIV viral load and CD4 T cell count in infected subjects; with an emphasis on viral replication in co-infection
Hossein Rahimi
1
Seyyed Abdolrahim Rezaee
2
Narges Valizade
3
Rosita Vakili
rosita_vakily@yahoo.com
4
Houshang Rafatpanah
5
Hematology Department, Ghaem Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Immunology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Inflammation and Inflammatory Diseases Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Inflammation and Inflammatory Diseases Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Inflammation and Inflammatory Diseases Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Objective(s): HTLV-I and HIV virus quantification is an important marker for assessment of virus activities. Since there is a direct relationship between the number of virus and disease progression, HTLV- I and HIV co-infection might have an influence on the development of viral associated diseases, thus, viral replication of these viruses and co-infection were evaluated. Materials and Methods: In this study, 40 subjects were selected; 14 HIV infected, 20 HTLV-I infected and 6 HTLV-I/HIV co-infected subjects. The amount of viruses was measured using qPCR TaqMan method and CD4 and CD8 lymphocytes were assessed by flow cytometry. Results: The mean viral load of HIV infected subjects and HTLV-I infected individuals were 134626.07±60031.07 copies/ml and 373.6±143.3 copies/104 cells, respectively. The mean HIV viral load in co-infected group was 158947±78203.59 copies/ml which is higher than HIV infected group. The mean proviral load of HTLV-I in co-infected group was 222.33±82.56 copies/ml which is lower than HTLV-I infected group (P<0.05). Also, the mean white blood cell count was higher in co-infected group (5666.67±1146.49 cells/μl). However, the differences between these subjects did not reach to a statistical significance within 95% confidence interval level (P =0.1). No significant differences were observed regarding CD4 and CD8 positive lymphocytes between these groups. Conclusion: HTLV-I/HIV co-infection might promote HIV replication and could reduce the HTLV-I proviral load, in infected cells. Considering the presence of both viruses in Khorasan provinces, it encourages researchers and health administrators to have a better understanding of co-infection outcome.
https://ijbms.mums.ac.ir/article_2155_6da8813d0d65a011da991e756fd241ba.pdf
HIV viral load
HTLV-I/HIV co-infection
HTLV-I proviral load
Lymphocytes
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2014-01-01
17
1
55
61
10.22038/ijbms.2014.2156
2156
Effects of administration of histamine and its H1, H2, and H3 receptor antagonists into the primary somatosensory cortex on inflammatory pain in rats
Esmaeal Tamaddonfard
e.tamaddonfard@urmia.ac.ir
1
Nasrin Hamzeh Gooshchi
2
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Objective(s): The present study investigated the effects of microinjection of histamine and histamine H1, H2, and H3 receptor antagonists, chlorpheniramine, ranitidine and thioperamide, respectively into the primary somatosensory cortex (PSC) on inflammatory pain. Material and Methods: Two stainless steel guide canulas were bilaterally implanted into the PSC of anaesthetized rats. Inflammatory pain was induced by subcutaneous (SC) injection of formalin (50 μl, 2.5%) in the ventral surface of right hind paw. Time durations of licking/biting of the injected paw were recorded as a pain measure. Results: Formalin produced a biphasic pattern of licking/biting of the injected paw. Histamine at doses of 0.5, 1, and 2 μg decreased the intensity of pain. Chlorpheniramine and ranitidine at the same doses of 1 and 4 μg had no effects, whereas thioperamide at a dose of 4 μg suppressed both phases of formalin-induced pain. Pretreatments with chlorpheniramine and ranitidine at the same dose of 4 μg prevented histamine (2 μg)-induced antinociception. Antinociceptive effects were observed when thioperamide at doses of 1 and 4 μg was used with 0.25 and 1 μg of histamine, respectively. The antinociceptive effects induced by histamine (2 μg) and thioperamide (4 μg) were prevented by prior treatment with naloxone (4 μg). Conclusion: These results indicate that at PSC levels, histamine through post-synaptic H1, H2, and pre-synaptic H3 receptors might be involved in pain modulation. The endogenous opioid system may be involved in histamine- and thioperamide-induced antinociception.
https://ijbms.mums.ac.ir/article_2156_18e42498159d1a8e3deee6c9fbbdd497.pdf
Formalin-induced pain Histamine
Histamine receptor antagonists
Primary somatosensory cortex
Rats
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2014-01-01
17
1
62
68
10.22038/ijbms.2014.2157
2157
Effect of whole body vibration therapy on circulating serotonin levels in an ovariectomized rat model of osteoporosis
Li Huang
604272074@qq.com
1
Xian Hong Chen
2
Hai-Bin Wang
3
Wei-Shan Sun
4
Shao Chuan Huo.
5
Zi Qi Li
6
Wei-Min Deng
7
1Department of Rehabilitation, General Hospital of Guangzhou Military Command of PLA, Guangzhou, China
1Department of Rehabilitation, General Hospital of Guangzhou Military Command of PLA, Guangzhou, China
2Key Laboratory of Orthopaedics & Traumatology, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
1Department of Rehabilitation, General Hospital of Guangzhou Military Command of PLA, Guangzhou, China
2Key Laboratory of Orthopaedics & Traumatology, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
2Key Laboratory of Orthopaedics & Traumatology, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
1Department of Rehabilitation, General Hospital of Guangzhou Military Command of PLA, Guangzhou, China
Objective(s): Studies have reported that whole body vibration (WBV) played a vital role in bone remodeling. Circulating serotonin is also involved in negative regulating bone mass in rodents and humans. However, both WBV and inhibition of serotonin biosynthesis may suppress receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclastogenesis in vitro . The purpose of the current study was to investigate the effect of WBV therapy on the levels of serum serotonin in ovariectomized rats. Materials and Methods: Thirty-six-month-old female Sprague Dawley rats weighing 276.15±37.75 g were ovariectomized to induce osteoporosis, and another ten rats underwent sham operation to establish sham control (SHAM) group. After 3 months, ovariectomized rats were divided into three subgroups and then separately treated with WBV, Alendronate (ALN) and normal saline (OVX), SHAM group was given normal saline. After 6 weeks of treatment, rats were sacrificed. Serum serotonin, RANKL, bone turnover markers, and bone mineral density (BMD), bone strength were evaluated. Results: The serum serotonin level was significantly lower in WBV group than OVX and ALN groups (PP<0.001). RANKL levels significantly decreased in WBV and ALN groups compared to OVX group (PPP Conclusion: These data indicated that WBV enhanced the bone strength and BMD in ovariectomized rats most likely by reducing the levels of circulating serotonin
https://ijbms.mums.ac.ir/article_2157_93ee0de85178735ba8ee42a62d00e329.pdf
Osteoporosis
Ovariectomy
Serotonin
Whole body vibration
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2014-01-01
17
1
69
72
10.22038/ijbms.2014.2159
2159
Nephroprotective effects of Feijoa Sellowiana leaves extract on renal injury induced by acute dose of ecstasy (MDMA) in mice
Mohammad Karami
1
Faezeh Karimian Nokabadi
2
Mohammad Ali Ebrahimzadeh
3
Farshad Naghshvar
4
Department of Toxicopharmacology, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Department of Toxicopharmacology, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Department of Chemistry, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Department of Pathology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Objective(s): Because of the high total phenolic contents of Feijoa sellowiana , its nephroprotective effect was determined in 3,4-methylene dioxymethamphetamine (MDMA) treated mice. Materials and Methods: Animals received 10, 20 and 40 mg/kg doses of aqueous or methanol extract of F. sellowiana leaves, intra-peritoneally. After one hour, an acute administration of MDMA (20 mg/kg, IP) was injected. Nephroprotective effect was assayed by determination of serum creatinine, serum urea and kidney glutathione level. Histopathological study was also used. Results : Both extracts at 40 mg/kg resulted in a significant reversal in the raised serum creatinine levels (P <0.05). Not statistically significant was observed in effect of two extracts (P>0.05). A decrease in urea/ creatinine ratio was observed following aqueous extract treatment. Methanolic extract showed higher activity in increasing kidney glutathione (P Conclusion: F. sellowiana extract resulted in a markedly decrease in the nephrotoxicity of MDMA in mice.
https://ijbms.mums.ac.ir/article_2159_e11f36271ecbbe0510efc3d2918fcea9.pdf
Feijoa sellowiana
Kidney glutathione
MDMA
Nephrotoxicity
eng
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
2014-01-01
17
1
73
80
10.22038/ijbms.2014.2160
2160
Attenuation of N-nitrosodimethylamine induced hepatotoxicity by Operculina turpethum in Swiss Albino mice
Veena Sharma
1
Manu Singh
2
Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, India
Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, India
Objective(s): To appraise the antihepatotoxic efficacy of ethanolic extract of Operculum turpethum root on the liver of Swiss albino mice. Materials and Methods: Hepatic fibrosis was induced in adult male albino mice through intraperitoneal administrations of N-nitrosodimethylamine (NDMA) at the concentration of 10 mg/kg body weight. The liver toxicity and therapeutic effect of the plant ethanolic extract was assessed by the analysis of liver marker enzymes and antioxidant enzymes and liver histopathological studies. Results: Hepatotoxicity was manifested by significantly decreased (P <0.01) levels of the activities of the enzymatic and non enzymatic antioxidants such as superoxide dismutase, catalase, GSH and increased levels of cholesterol, AST, ALT, ALP and lipid peroxidation. The plant extract significantly restored the antioxidant enzyme level in the liver and exhibited significant dose dependent curative effect against NDMA induced toxicity which was also supported by histopathological studies of the liver. Conclusion: O. turpethum manifested therapeutic effects by significantly restoring the enzymatic levels and reducing the hepatic damage in mice. This work intends to aid researchers in the study of natural products which could be useful in the treatment of liver diseases including cancer.
https://ijbms.mums.ac.ir/article_2160_3bdcffae386f5d708a67569cf74c8aca.pdf
Hepatotoxicity
Liver
N-nitrosodimethylamine Operculina turpethum