%0 Journal Article %T Trans-chalcone enhances insulin sensitivity through the miR-34a/SIRT1 pathway %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Karimi Sales, Elham %A Jeddi, Sajad %A Ebrahimi-Kalan, Abbas %A Alipour, Mohammad Reza %D 2018 %\ 04/01/2018 %V 21 %N 4 %P 359-363 %! Trans-chalcone enhances insulin sensitivity through the miR-34a/SIRT1 pathway %K Liver %K MiR-34a %K Rat %K SIRT1 %K Trans-chalcone %R 10.22038/ijbms.2018.24300.6063 %X Objective(s): Trans-chalcone as the parent member of the chalcone series reduces circulating levels of insulin and glucose. However, the cellular mechanism of these effects is poorly understood. Sirtuin 1 (SIRT1) as a direct target of miR-34a controls homeostasis of glucose, and also improves insulin sensitivity. Therefore, the present study for the first time investigated the influence of trans-chalcone on the miR-34a/SIRT1 pathway as a possible mechanism for its hypoglycemic and hypoinsulinemic effects. Materials and Methods: In this study, thirty male rats were randomly divided into three groups (n=10): solvent control (NS), oral administration of trans-chalcone for 2 (N2T) and 6 weeks (N6T) groups. Then, hepatic levels of miR-34a and SIRT1 were measured through the qRT-PCR method.Results: Trans-chalcone reduced food intake, body weight gain, and serum glucose as well as insulin levels. Also, this chalcone inhibited hepatic miR-34a expression and significantly elevated SIRT1 mRNA level. Conclusion: Trans-chalcone as an insulin-sensitizing chalcone partly acts through the miR-34a/SIRT1 pathway. %U https://ijbms.mums.ac.ir/article_10332_61c5d41bd0c99e547af5ff711d7c5e26.pdf