%0 Journal Article %T Insulin glargine affects the expression of Igf-1r, Insr, and Igf-1 genes in colon and liver of diabetic rats %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Juárez-Vázquez, Clara I %A Gurrola-Díaz, Carmen M %A Vargas-Guerrero, Belinda %A Domínguez-Rosales, José A %A Rodriguez-Ortiz, Jessica Fabiola %A Barros-Núñez, Patricio %A Flores-Martínez, Silvia E %A Sánchez-Corona, José %A Rosales-Reynoso, Mónica A %D 2018 %\ 05/01/2018 %V 21 %N 5 %P 489-494 %! Insulin glargine affects the expression of Igf-1r, Insr, and Igf-1 genes in colon and liver of diabetic rats %K Colon %K Diabetes %K Insulin glargine %K Liver %K Metformin %K NPH insulin %K Rats %R 10.22038/ijbms.2018.24867.6185 %X Objective(s): The mitogenic effect of the analogous insulin glargine is currently under debate since several clinical studies have raised the possibility that insulin glargine treatment has a carcinogenic potential in different tissues. This study aimed to evaluate the Igf-1r, Insr, and Igf-1 gene expression in colon and liver of streptozotocin-induced diabetic rats in response to insulin glargine, neutral protamine Hagedorn (NPH) insulin, and metformin treatments. Materials and Methods: Male Wistar rats were induced during one week with streptozotocin to develop Type 2 Diabetes (T2D) and then randomly distributed into four groups. T2D rats included in the first group received insulin glargine, the second group received NPH insulin, the third group received metformin; finally, untreated T2D rats were included as the control group. All groups were treated for seven days; after the treatment, tissue samples of liver and colon were obtained. Quantitative PCR (qPCR) was performed to analyze the Igf-1r, Insr and Igf-1 gene expression in each tissue sample. Results: The liver tissue showed overexpression of the Insr and Igf-1r genes (P>0.001) in rats treated with insulin glargine in comparison with the control group. Similar results were observed for the Insr gene (P>0.011) in colonic tissue of rats treated with insulin glargine. Conclusion: These observations demonstrate that insulin glargine promote an excess of insulin and IGF-1 receptors in STZ-induced diabetic rats, which could overstimulate the mitogenic signaling pathways. %U https://ijbms.mums.ac.ir/article_10542_7e264878e6e9c46dab650b5d7c6bc9c3.pdf