%0 Journal Article %T Naphthoquinones from Handroanthus impetiginosus promote skin wound healing through Sirt3 regulation %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Ahmad, Fayyaz %A Bibi, Shaheen %A Kang, Mincheol %A Anees, Mariam %A Ansar, Muhammad %A Alam, Muhammad Rizwan %A Kim, Sun %A Wahedi, Hussain Mustatab %D 2020 %\ 09/01/2020 %V 23 %N 9 %P 1139-1145 %! Naphthoquinones from Handroanthus impetiginosus promote skin wound healing through Sirt3 regulation %K Beta %K Lapachone Dermatology Inflammation Regeneration Tabebuia %R 10.22038/ijbms.2020.43706.10275 %X Objective(s): Lapachone is a natural naphthoquinone-derived compound found in Tabebuia avellanedae. It is well-known for its analgesic, anti-inflammatory, anti-microbial, diuretic, and anti-cancerous effects. However, the wound-healing effects of this compound are not known yet. The aim of this study was to investigate the wound healing activity of naphthoquinones (α-lapachone and β-lapachone) from Handroanthus impetiginosus. Materials and Methods: Expression of Sirt3, migration-related proteins (Rac1, Cdc42, α-Pak) and angiogenesis-related protein of vascular endothelial growth factor (VEGF) was monitored using western blot analysis. Blood vessel formation and tissue development were monitored by angiogenesis assay and hematoxylin & eosin (H & E) staining, respectively on mouse skin tissue samples. Both α-lapachone and β-lapachone increased Sirt3 expression in vivo, but only β-lapachone increased Sirt3 expression in vitro. Results: Both the compounds accelerated wound healing in cultured skin cells as well as mouse skin; however, β-lapachone was more effective at lower concentrations. Both of the compounds increased the expression of migration-related proteins both in vitro and in vivo. Similarly, α-lapachone and β-lapachone increased VEGF expression, tissue development and blood vessel formation in mouse skin.Conclusion: These findings indicated that α-lapachone and β-lapachone are novel Sirt3 activators, and Sirt3 has a role in wound healing. Thus, Sirt3 and its regulators come out as a novel target and potential drug candidates, respectively in the important field of cutaneous wound healing. %U https://ijbms.mums.ac.ir/article_16139_c2c8bb6f3e32bc19e9c5dd1f36db3e9f.pdf