%0 Journal Article %T Evaluation of immune response after moderate and overtraining exercise in wistar rat %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Gholamnezhad, Zahra %A Boskabady, Mohammad Hossein %A Hossein, Mahmoud %A Sankian, Mojtaba %A Khajavi Rad, Abolfazl %D 2014 %\ 01/01/2014 %V 17 %N 1 %P 1-8 %! Evaluation of immune response after moderate and overtraining exercise in wistar rat %K Immune system %K Moderate exercise %K Overtraining exercise %K Rat %K Th1 %K Th2 %R 10.22038/ijbms.2014.2148 %X   Objective(s): The effect of prolonged overtraining on cytokine kinetics was compared with moderate exercise in the present study.   Materials and Methods: Male Wistar rats were randomly divided into control sedentary (C), moderate trained (MT), (V=20 m/min, 30 min/day for 6 days a week, 8 weeks), overtrained (OT) (V=25 m/min, 60min/day for 6 days a week, 11 weeks) and recovered overtrained (OR) (OT plus 2 weeks recovery) groups, (n=6 for each group). Immediately, 24 hr and 2 weeks (in OR) after last bout of exercise blood samples were obtained. The plasma concentrations of TNFα, IL-6, IL-10, IL-4 and IFN were measured by ELISA method. Results: Immediately after last bout of exercise the following findings were observed; IL-6, IL-10 and TNFαconcentrations increased in OT and OR groups compared with control (P<0.05–P<0.001). Serum level of IL-4 decreased (P<0.01) but IFN increased (P<0.05) in MT group vs. control. In addition, circulatory levels of TNFα IL-6, IL-10 and IL-4 were higher but the IFN concentrations were lower in OT and OR groups than MT group (P<0.05-P<0.01). The IFN-γIL4 ratio was significantly increased in MT (P<0.01) while it decreased in OT group. There were not statistical differences in TNFα IL-6, and IFN levels between different time intervals after exercise in MT, OT and OR groups. Conclusion: These data confirm a positive effect of moderate exercise on immune function and a decrease in susceptibility to viral infection by inducing Th1 cytokine profile shift. However, prolonged and overtraining exercise causes numerous changes in immunity that possibly reflects physiological stress and immune suppression. %U https://ijbms.mums.ac.ir/article_2148_9ff0a6a6b98ee77c5507864de248c221.pdf