%0 Journal Article %T Mild hypothermia reduces expression of Fas/FasL and MMP-3 after cerebral ischemia-reperfusion in rats %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Zhao, Jingkun %A Duan, Shurong %A Zhou, Jinxia %A Sun, Ruihong %A Zhang, Liming %A Wang, Desheng %D 2014 %\ 06/01/2014 %V 17 %N 6 %P 454-459 %! Mild hypothermia reduces expression of Fas/FasL and MMP-3 after cerebral ischemia-reperfusion in rats %K Cerebral ischemia/reperfusion %K Fas %K FasL %K Hypothermia %K MACO rat %K MMP-3 %R 10.22038/ijbms.2014.2931 %X Objective(s):To investigate the effects of local mild hypothermia on the expression of Fas, FasL and MMP-3 after cerebral ischemia-reperfusion in rats. Materials and Methods:Male Wistar rats were divided into sham-operated group (Sham), normothermia group (NT), and hypothermia group (HT). MCAO/R model was established by Longa’s method, and reperfusion was allowed after 2 hr occlusion. Mild hypothermia (33±0.5℃) for 6 hr was initiated at the start of reperfusion. Immunohistochemistry was performed to determine expression Fas, FasL, and MMP-3. Results: Infarct volume was reduced in the hypothermia group (18.43±4.23%) compared with the normothermia group (24.76±5.76%) (P<0.05). In mild hypothermia group, numbers of Fas-positive and MMP-3 positive cells were significantly less than those of normothermia group (P<0.05). Neurological functional scores of mild hypothermia were significantly improved (P<0.05). Conclusion: Mild hypothermia decreases infarct volume after cerebral ischemia-reperfusion, reduces Fas and MMP-3 expression, but increases FasL in cerebral ischemia-reperfusion rats. %U https://ijbms.mums.ac.ir/article_2931_b923cc1f5f757de29207887f9de1a18c.pdf