%0 Journal Article %T Difference in transient ischemia-induced neuronal damage and glucose transporter-1 immunoreactivity in the hippocampus between adult and young gerbils %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Min Park, Seung %A Lee, Jae-Chul %A Hui Chen, Bai %A Shin, Bich-Na %A Hwi Cho, Jeong %A Hye Kim, In %A Ha Park, Joon %A Won, Moo-Ho %A Hyeon Ahn, Ji %A Tae, Hyun-Jin %A Cheol Shin, Myoung %A Woo Park, Chan %A Hwi Cho, Jun %A Young Lee, Hui %D 2016 %\ 05/01/2016 %V 19 %N 5 %P 521-528 %! Difference in transient ischemia-induced neuronal damage and glucose transporter-1 immunoreactivity in the hippocampus between adult and young gerbils %K CA1 region %K Delayed neuronal death %K Ischemia/reperfusion injury Pyramidal neurons %K Young gerbil %R 10.22038/ijbms.2016.6937 %X Objective(s): The alteration of glucose transporters is closely related with the pathogenesis of brain edema. We compared neuronal damage/death in the hippocampus between adult and young gerbils following transient cerebral ischemia/reperfusion and changes of glucose transporter-1(GLUT-1)-immunoreactive microvessels in their ischemic hippocampal CA1 region. Materials and Methods: Transient cerebral ischemia was developed by 5-min occlusion of both common carotid arteries. Neuronal damage was examined by cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B histofluorescence staining and changes in GLUT-1 expression was carried out by immunohistochemistry. Results: About 90% of pyramidal neurons only in the adult CA1 region were damaged after ischemia/reperfusion; in the young, about 53 % of pyramidal neurons were damaged from 7 days after ischemia/reperfusion. The density of GLUT-1-immunoreactive microvessels was significantly higher in the young sham-group than that in the adult sham-group. In the ischemia-operated-groups, the density of GLUT-1-immunoreactive microvessels was significantly decreased in the adult and young at 1 and 4 days post-ischemia, respectively, thereafter, the density of GLUT-1-immunoreactive microvessels was gradually increased in both groups after ischemia/reperfusion. Conclusion: CA1 pyramidal neurons of the young gerbil were damaged much later than that in the adult and that GLUT-1-immunoreactive microvessels were significantly decreased later in the young. These data indicate that GLUT-1 might differently contribute to neuronal damage according to age after ischemic insults. %U https://ijbms.mums.ac.ir/article_6937_5a27eb6ae681a63a82faf2ec4d265eaf.pdf