Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Bio-effectiveness of the main flavonoids of Achillea millefolium in the pathophysiology of neurodegenerative disorders- a review
604
612
EN
Fatemeh
Ayoobi
Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Ali
Shamsizadeh
0000-0001-8329-9156
Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
alishamsy@gmail.com
Iman
Fatemi
Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Alireza
Vakilian
Geriatric Care Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
alirezavakilian7@gmail.com
Mohammad
Allahtavakoli
Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
alahtavakoli@razi.tums.ac.ir
Gholamhossein
Hassanshahi
Pistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
mojgan.noroozi@yahoo.com
Amir
Moghadam-Ahmadi
Department of Neurology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
a_moghadamahmadi@yahoo.com
10.22038/ijbms.2017.8827
The<em> Achillea millefolium </em>L. (Yarrow) is a common herb which is widely being used, worldwide. <em>Achillea</em> is being used for treatment of many disorders since centuries. It is considered safe for supplemental use and flavonoids such as kaempferol, luteolin and apigenin are of main constituents present in <em>Achillea</em>. Most of both antioxidant and anti-inflammatory properties of this herb have been attributed to its flavonoid content. Oxidative and inflammatory processes play important roles in pathogenesis of neurodegenerative diseases. Present review was aimed to review the latest literature evidences regarding application of <em>Achillea</em> and/or its three main flavonoid constituents on epilepsy, Alzheimer's disease, multiple sclerosis, Parkinson's disease and stroke.
Achillea millefolium,Flavonoids,Medicinal,Neurodegenerative disease,Plants
https://ijbms.mums.ac.ir/article_8827.html
https://ijbms.mums.ac.ir/article_8827_8bd130a08c797731f218f0ebdac209ca.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Thiadiazoles: the appropriate pharmacological scaffolds with leishmanicidal and antimalarial activities: a review
613
622
EN
Azar
Tahghighi
0000-0002-1221-4490
Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran
atahghighi2009@gmail.com
Fateme
Babalouei
Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran
10.22038/ijbms.2017.8828
Leishmaniasis and malaria are serious public health problems in tropical and sub-tropical regions worldwide. Development of drug-resistant strains has disrupted efforts to control the spread of these diseases in the world. The conventional antiparasitic chemotherapy still suffers from side effects and drug resistance. Therefore, the development of novel antimalarial and leishmanicidal drugs remains a critical topic to combat against these diseases. Five-membered heterocyclic systems have possessed antiparasitic activity such as thiadiazole scaffold which is a prevalent and an important heterocyclic ring. For this purpose, the authors introduce a series of synthetic thiadiazole derivatives with antileishamanial activity. Also, the authors searched a number of sources and articles to find thiadiazole derivatives with antileishamnial and antimalarial activity. Then all of the findings were reviewed. 5-nitroheteroaryl-1,3,4-Thiadiazole derivatives with different substituents at position 2 of the thiadiazole ring (8, 10-11) presented the best antileishmanial activity with low toxicity compared with reference drug. Also, 1,3,4-thiadiazole-2-sulfonamide derivative (18) showed excellent inhibitory activity against<em> pf</em>CA as a special enzyme in <em>Plasmodium falciparum.</em> Thiadiazole scaffold has the suitable physicochemical and pharmacokinetic properties and still stays as a therapeutic target for the development of a novel lead in the medicinal chemistry. Therefore, the current review provides a brief summary of medicinal chemistry of thiadiazole ring and introduces novel leads possessing this nucleus with antimalarial and antileishmanial activities.v
Antileishmanial,Amastigote,Antimalarial,Plasmodium,Promastigote,Thiadiazole
https://ijbms.mums.ac.ir/article_8828.html
https://ijbms.mums.ac.ir/article_8828_bb0d14740cc7290ae9e882f37699a366.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Effects of left prefrontal transcranial direct current stimulation on the acquisition of contextual and cued fear memory
623
630
EN
Shahsanam
Abbasi
Institute for Cognitive Science Studies (ICSS), Tehran, Iran
shahsanam2001@yahoo.com
Mohammad
Nasehi
Institute for Cognitive Science Studies (ICSS), Tehran, Iran
nasehi@iricss.org
Hamid Reza
Soleimanpour Lichaei
Department of Stem Cells and Regenerative Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Mohammad-Reza
Zarrindast
Institute for Cognitive Science Studies (ICSS), Tehran, Iran
10.22038/ijbms.2017.8829
<strong><em>Objective(s)</em></strong>: Behavioral and neuroimaging studies have shown that transcranial direct current stimulation, as a non-invasive neuromodulatory technique, beyond regional effects can modify functionally interconnected remote cortical and subcortical areas. In this study, we hypothesized that the induced changes in cortical excitability following the application of cathodal or anodal tDCS over the left frontal cortex as pre-training would affect functional connectivity in resting-state circuits of fear memory and consequently could improve or disturb the acquisition of fear memory.<br /> <strong><em>Materials and Methods: </em></strong>In order to evaluate the polarity-dependent effects of tDCS on the acquisition of fear memory and the functional connectivity, we applied left prefrontal anodal or cathodal stimulation at 200 μA for one session to healthy mice for the durations of 20 and 30 min prior to fear conditioning.<br /> <strong><em>Results:</em></strong> Our results revealed that the administration of left prefrontal anodal (for both 20 and 30 min durations) and cathodal (at 30 min duration) tDCS impaired the acquisition of both contextual and cued fear memory. In addition, we did not observe a direct correlation between stimulation duration and the efficacy of tDCS on the acquisition of contextual and cued fear memory.<br /> <strong><em>Conclusion:</em></strong> In this study, the impairments of both contextual and cued memory further confirmed the previous studies reporting that the administration of transcranial stimulation would affect the activity of deeper structures like amygdala and hippocampus as the main components of the fear memory circuit in acquisition, storage, and expression of the memory.
fear,Memory,Prefrontal cortex,tDCS
https://ijbms.mums.ac.ir/article_8829.html
https://ijbms.mums.ac.ir/article_8829_df018737e620d93d08f3a0aadaf1d41b.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Coumarin derivatives bearing benzoheterocycle moiety: synthesis, cholinesterase inhibitory, and docking simulation study
631
638
EN
Kimia
Hirbod
Department of Medical Chemistry, School of Pharmacy, International Campus, Tehran University of Medical Science, Tehran, Iran
kimiairan2014@gmail.com
Leili
Jalili-baleh
0000-0002-0532-718X
Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
leili.jalili@gmail.com
Hamid
Nadri
Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
hnadri@razi.tums.ac.ir
Seyed Esmaeil
Sadat
Ebrahimi
Department of Medical Chemistry, School of Pharmacy, International Campus, Tehran University of Medical Science, Tehran, Iran
Alireza
Moradi
Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
almoradi@yahoo.com
Bahar
Pakseresht
Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Alireza
Foroumadi
0000-0002-2043-8523
Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
afateh2@gmail.com
Abbas
Shafiee
Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
shafieea@tums.ac.ir
Mehdi
Khoobi
Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran, Iran
pmehdi.khoobi@gmail.com
10.22038/ijbms.2017.8830
<em><strong>Objective(s):</strong></em> To investigate the efficiency of a novel series of coumarin derivatives bearing benzoheterocycle moiety as novel cholinesterase inhibitors.<br /> <em><strong>Materials and Methods:</strong></em> Different 7-hydroxycoumarin derivatives were synthesized via Pechmann or Knoevenagel condensation and conjugated to different benzoheterocycle (8-hydroxyquinoline, 2-mercaptobenzoxazole or 2-mercaptobenzimidazole) using dibromoalkanes 3a-m. Final compounds were evaluated against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) by Ellman's method. Kinetic study of AChE inhibition and ligand-protein docking simulation were also carried out for the most potent compound 3b.<br /> <em><strong>Results:</strong> </em>Some of the compounds revealed potent and selective activity against AChE. Compound 3b containing the quinoline group showed the best activity with an IC50 value of 8.80 µM against AChE. Kinetic study of AChE inhibition revealed the mixed-type inhibition of the enzyme by compound 3b. Ligand-protein docking simulation also showed that the flexibility of the hydrophobic five carbons linker allows the quinoline ring to form π-π interaction with Trp279 in the PAS.<br /> <em><strong>Conclusion:</strong></em> We suggest these synthesized compounds could become potential leads for AChE inhibition and prevention of AD symptoms.
acetylcholinesterase,Alzheimer’s disease,Benzoheterocycles Butyrylcholinesterase,Coumarin
https://ijbms.mums.ac.ir/article_8830.html
https://ijbms.mums.ac.ir/article_8830_b043e8e9f0c14364270454802e242e00.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Novel cilostamide analogs, phosphodiesterase 3 inhibitors, produce positive inotropic but differential lusitropic and chronotropic effects on isolated rat atria
639
647
EN
Azar
Hosseini
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
hoseinia@mums.ac.ir
Reza
Shafiee-Nick
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
shafieer@mums.ac.ir
Hamid
Sadeghian
Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran
sadeghianh@mums.um.ac.ir
Heydar
Parsaee
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
10.22038/ijbms.2017.8831
<strong><em>Objective(s):</em></strong> Recently, we showed that some new synthetic compounds structurally related to cilostamide (4-(1,2-dihydro-2-oxoquinolin-6-hydroxy)- N-cyclohexyl-N-methylbutanamide), a selective phosphodiesterase 3 (PDE3) inhibitor, produce inotropic effect comparable to that of IBMX (3-isobutyl-1-methylxanthine), a non-selective PDE inhibitor, but with differential chronotropic effect. In this investigation, we compared the pharmacological effects of these compounds as potential cardiotonic agents using the spontaneously beating atria model.<br /> <strong><em>Materials and Methods: </em></strong>In each experiment, rats were treated with reserpine. The atrium was isolated and mounted in an organ bath. We assessed chronotropic and inotropic effects using cumulativelogconcentration-response curves of isoprenaline alone or in combination of each test-compound.<br /> <strong><em>Results: </em></strong>Majority of test compounds augment atria contraction force (ACF) significantly but with different potencies on atrium contraction rate. Cilostamide, MCPIP ([4-(4-methyl piperazin-1-yl)-4-oxobutoxy)-4-methylquinolin-2(1H)-one]), methyl carbostyril compounds- (mc1), mc2 and mc5 increased the isoprenaline effect on ACF synergistically. But, mc6 failed to potentiate the effect of isoprenalin; mc3 and mc4 did not increase ACF, which may be because of their higher hydrophilic nature. It was interesting that mc2, alone or in combination with isoprenaline, produced the highest inotropic effect while it did not affect the basal contraction rate and almost blocked the isoprenaline chronotropic effect.<br /> <strong><em>Conclusion: </em></strong>Combination of mc2 with isoprenaline had synergistic effect on inotropic effect, but this combination reduced isoprenaline chronotropic effect; therefore, these effects cannot be related to reducing B-adrenergic receptors activity. These compounds showed different effects; probably all of them were not mediated via PDE3 inhibition and other mechanisms are involving.
Cilostamide,Inotropic activity,Isoprenaline,PDE inhibitor,Rat atria
https://ijbms.mums.ac.ir/article_8831.html
https://ijbms.mums.ac.ir/article_8831_704ac3e62c625e9f55df49a09bccd0eb.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Cxcr4 expression is associated with time–course permanent and temporary myocardial infarction in rats
648
654
EN
Ali Asghar
Kiani
Razi Herbal Medicines Research Center and School of Allied Medical Sciences, Department of Hematology and Blood Transfusion, Lorestan University of Medical Sciences, Khorramabad, Iran
aliasgharkianii@gmail.com
Fereshteh
Babaei
Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
Mehrnoosh
Sedighi
0000-0002-2002-2413
Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
m.sedighi63@ymail.com
Azam
Soleimani
0000-0002-3100-1706
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
asoleymani2008@yahoo.com
Kolsum
Ahmadi
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Somayeh
Shahrokhi
Department of Immunology, Lorestan University of Medical Sciences, Khorramabad, Iran
shahrokhi_so@yahoo.com
Khatereh
Anbari
Department of Social Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
dr.anbari@gmail.com
Afshin
Nazari
0000-0002-1863-1092
Razi Herbal Medicines Research Center, Department of Physiology, Lorestan University of Medical Sciences, Khorramabad, Iran
nazary257@yahoo.com
10.22038/ijbms.2017.8832
<strong><em>Objective(s)</em></strong>: Experimental myocardial infarction triggers secretion of Stromal cell-derived factor1 and lead to increase in the expression of its receptor "CXCR4" on the surface of various cells. The aim of this study was to evaluate the expression pattern of CXCR4 in peripheral blood cells following time-course permanent and temporary ischemia in rats.<br /> <strong><em>Materials and Methods: </em></strong>Fourteen male Wistar rats were divided into two groups of seven and were placed under permanent and transient ischemia. Peripheral blood mononuclear cells were isolated at different time points, RNAs extracted and applied to qRT-PCR analysis of the CXCR4 gene. <br /> <strong><em>Results:</em></strong> Based on repeated measures analysis of variance, the differences in the expression levels of the gene in each of the groups were statistically significant over time (the effect of time) (<em>P-value</em>< 0.001). Additionally, the difference in gene expression between the two groups was statistically significant (the effect of group), such that at all times, the expression levels of the gene were significantly higher in the permanent ischemia than in the transient ischemia group (<em>P-value</em><0.001). Moreover, the interactive effect of time-group on gene expression was statistically significant (<em>P-value</em><0.001).<br /> <strong><em>Conclusion:</em></strong> CXCR4 is modulated in an induced ischemia context implying a possible association with myocardial infarction. Checking of CXCR4 expression in the ischemic changes shows that damage to the heart tissue trigger the secretion of inflammatory chemokine SDF, Followed by it CXCR4 expression in blood cells. These observations suggest that changes in the expression of CXCR4 may be considered a valuable marker for monitoring myocardial infarction.
CXCR4 gene expression,Time-course,Permanent cardiac ischemia,Temporary cardiac ischemia
https://ijbms.mums.ac.ir/article_8832.html
https://ijbms.mums.ac.ir/article_8832_3f337ad43cecb8967e98683cdc800d23.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Melatonin upregulates ErbB1 and ErbB4, two primary implantation receptors, in pre-implantation mouse embryos
655
661
EN
Ghazaleh
Moshkdanian
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
g_moshkdanian@yahoo.com
Fatemeh
Moghani-Ghoroghi
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
fatima_moghani@yahoo.com
Parichehr
Pasbakhsh
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Seyed Noureddin
Nematollahi-Mahani
Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Atefeh
Najafi
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iranv
najafiat@gmail.com
Iraj
Ragerdi Kashani
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
ragerdi@sina.tums.ac.ir
10.22038/ijbms.2017.8833
<strong><em>Objective(s):</em></strong> To evaluated the effects of melatonin on early embryo competence and the expression rate of the primary implantation receptors (ErbB1 and ErbB4).<br /> <strong><em>Materials and Methods: </em></strong>Two-cell mouse embryos were cultured in 3 groups: simple media, melatonin-treated (10<sup>-9</sup> M melatonin) and Luzindole-treated (10<sup>-9</sup> M luzindole). Then, the rate of ErbB1 and ErbB4 gene and protein expression, the level of intracellular ROS, antioxidant capacity, and also the number of cells were evaluated and compared with the fourth group <em>in vivo</em> developed blastocysts (control group).<br /> <strong><em>Results:</em></strong> We concluded that melatonin significantly up-regulated the ErbB1 and ErbB4 gene and protein expression, decreased intracellular ROS, increased the total antioxidant capacity, and also elevated the cell numbers in the melatonin-treated group compared with the other groups (<em>P≤</em>0.05).<br /> <strong><em>Conclusion:</em></strong> The use of melatonin may be a helpful factor in improving the embryo quality and enhancing the expression of ErbB1 and ErbB4, two important implantation-related genes and proteins.
Antioxidant,ErbB1,ErbB4,Melatonin,Pre-implantation embryo,Reactive Oxygen Species
https://ijbms.mums.ac.ir/article_8833.html
https://ijbms.mums.ac.ir/article_8833_120455d16174bceb3f8f543cfdefa167.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Establishment of human hair follicle mesenchymal stem cells with overexpressed human hepatocyte growth factor
662
675
EN
Dan
Zhou
Department of Pediatrics, The Second Hospital of Jilin University, Changchun 130041, China
770700496@qq.com
Hongjing
Cheng
Department of Gastroenterology, The First Hospital of Jilin University, Changchun 130041, China
Jinyu
Liu
The Ministry of Education Key Laboratory, The College of Basic Medical Sciences, of Jilin University, Changchun 130041, China
mxwmengxiangwei@163.com
Lei
Zhang
Department of Pediatrics, The Second Hospital of Jilin University, Changchun 130041, China
1169329244@qq.com
10.22038/ijbms.2017.8834
<strong><em>Objective(s)</em></strong>: Chronic liver disease has become a major health problem that causes serious damage to human health. Since the existing treatment effect was not ideal, we need to seek new treatment methods.<br /> <strong><em>Materials and Methods: </em></strong>We utilized the gene recombination technology to obtain the human hair mesenchymal stem cells which overexpression of human hepatocyte growth factor (hHGF). Furthermore, we verified the property of transfected cells through detecting surface marker by flow cytometry.<br /> <strong><em>Results:</em></strong> We show here establishment of the hHGF-overexpressing lentivirus vector, and successfully transfection to human hair follicle mesenchymal stem cells. The verified experiments could demonstrate the human hair follicle mesenchymal stem cells which have been transfected still have the properties of stem cells.<br /> <strong><em>Conclusion:</em></strong> We successfully constructed human hair follicle mesenchymal stem cells which overexpression hHGF, and maintain the same properties compared with pro-transfected cells.
Hair follicle,HGF,Lentivirus,Liver diseases,Stem cells
https://ijbms.mums.ac.ir/article_8834.html
https://ijbms.mums.ac.ir/article_8834_8196218be98684140fa62d5500c2fc2d.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Allicin attenuates tunicamycin-induced cognitive deficits in rats via its synaptic plasticity regulatory activity
676
682
EN
Qiong
Xiang
Institute of Medicine, Medical Research Center, Jishou University, Hunan, China
Xian-hui
Li
Institute of Medicine, Medical Research Center, Jishou University, Hunan, China
lxhsurgeon@126.comv
Bo
Yang
Jishou University First Affiliated Hospital, Jishou University, Hunan, China
boyangyb@163.com
Xin-xing
Fang
Institute of Medicine, Medical Research Center, Jishou University, Hunan, China
Jing
Jia
Institute of Medicine, Medical Research Center, Jishou University, Hunan, China
Jie
Ren
Institute of Medicine, Medical Research Center, Jishou University, Hunan, China
Yu-chun
Dong
Institute of Medicine, Medical Research Center, Jishou University, Hunan, China
Cheng
Ou-Yang
Institute of Medicine, Medical Research Center, Jishou University, Hunan, China
Guang-cheng
Wang
College of Chemistry and Chemical Engineering, Jishou University, Hunan, China
10.22038/ijbms.2017.8837
<strong><em>Objective(s)</em></strong>: To illuminate the functional effects of allicin on rats with cognitive deficits induced by tunicamycin (TM) and the molecular mechanism of this process.<br /> <strong><em>Materials and Methods: </em></strong>200–250 g male SD rats were divided into three groups at random: control group (n=12), TM group (5 μl, 50 μM, i.c.v, n=12), and allicin treatment group (180 mg/kg/d with chow diet, n=12). After 16 weeks of allicin treatment, the learning ability and memory were tested using novel object recognition (NOR) testing on rats with 72 hr TM treatment (5 μl, 50 μM, i.c.v); meanwhile, the variation of field excitatory postsynaptic potential (fEPSP) in the Schaffer Collateral (SC)-CA1 synapse was detected by extracellular electrophysiological recordings and the morphology of dendritic spine was observed by Golgi staining as well as detecting several synaptic plasticity-related proteins by Western blot.<br /> <strong><em>Results:</em></strong> The density of dendritic spine was increased significantly in allicin-treated groups and the correspondence slope of fEPSP in TM-induced cognitive deficits group was enhanced and expression of synaptophysin and glutamate receptor-1(GluR1) in hippocampal neurons was up-regulated.<br /> <strong><em>Conclusion</em></strong><strong><em>:</em></strong> The results indicate that allicin plays an important role in synaptic plasticity regulation. These finding showed that allicin could be used as a pharmacologic treatment in TM-induced cognitive deficits.
Allicin,Dendritic spine density,fEPSP,Hippocampus,Synaptic plasticity
https://ijbms.mums.ac.ir/article_8837.html
https://ijbms.mums.ac.ir/article_8837_0c2a8003af09d62c03b6c54501b936cf.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Orthotopic liver transplantation from cardiac death donors in the mouse: a new model and evaluation of cardiac death time
683
689
EN
Zhenzhen
Liu
Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China
zhzhll@126.com
Ning
Pan
Department of Anesthesiology, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China
nn_doctor@163.com
Xiangwei
Lv
Department of General Surgery, Anhui Provincial Hospital, Hefei, Anhui, China
dlmulxw@163.com
Song
Li
Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China
lslengfeng@126.com
Liming
Wang
Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China
wanglmdoctor@sina.com
Qinlong
Liu
Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China
qinlongliu@yeah.net
10.22038/ijbms.2017.8838
<strong><em>Objective(s)</em></strong>: The goal of this research was to develop a mouse orthotopic liver transplantation (LTx) model from donor-after-cardiac-death (DCD) grafts.<br /> <strong><em>Materials and Methods: </em></strong>Mice were randomly assigned to the experimental group or the sham group. The mice in the experimental group were divided into three groups according to the warm ischemia time (WIT) of liver graft: normal LTx, WIT 30 minute (min) +LTx and WIT 45 min +LTx. The descending aorta was clamped using a miniature aortic clamp to simulate cardiac arrest in the DCD grafts. Subsequently, the grafts were orthotopically transplanted into C57BL/6 mice. The 7-day survival rate, serum alanine aminotransferase (ALT), inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6) mRNA level, tumor necrosis factor-alpha (TNF-α) mRNA level, as well as hepatic pathologic alterations were observed.<br /> <strong><em>Results:</em></strong> The 7-day survival rate was markedly lower in the WIT 45 min+LTx group than that in the normal LTx group (25% versus 100%, <em>P</em>-value<0.05), with no significant difference between the WIT 30 min +LTx and normal LTx group (75% versus 100%, <em>P</em>-value>0.05). Serum ALT level of WIT 45 min+LTx group was markedly higher than that of normal LTx and WIT 30 min+LTx group (<em>P</em>-value<0.01). There were significant differences in necrosis and apoptosis among the three groups (<em>P</em>-value<0.05). The expression of iNOS, IL-6 mRNA and TNF-α mRNA in WIT 45 min +LTx group all increased significantly compared with the normal LTx and WIT 30 min+LTx group.<br /> <strong><em>Conclusion:</em></strong> The DCD LTx model is feasible in the mouse and would provide many advantages for biomedical research on LTx from DCD grafts.
Animal model,Liver transplantation,Primary graft dysfunction,Reperfusion injury,Warm ischemia time
https://ijbms.mums.ac.ir/article_8838.html
https://ijbms.mums.ac.ir/article_8838_c551c01a3c34f06cd59d8ee599c38946.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Neuroprotective role of curcumin on the hippocampus against the structural and serological alterations of streptozotocin-induced diabetes in sprague dawely rats
690
699
EN
Nermeen
Mohammed Faheem
Department of Anatomy and Embryology, Faculty of Medicine, Ain Shams University, Egypt
Ahmad
El Askary
Department of Medical Biochemistry, Faculty of Medicine (New Damietta), Al Azhar University, Egypt & Department of Clinical Laboratories, College of Applied Medical Sciences, Taif University, KSA
ahmadelaskary3@gmail.com
10.22038/ijbms.2017.8839
<strong><em>Objective(s)</em></strong>: Diabetes mellitus causes impaired memory and cognitive functions. The hippocampus plays a key role in memory and learning. Curcumin attenuates diabetic nephropathy <em>in vivo.</em> Curcumin has shown a neurogenic effect and cognition-enhancing potential in aged rats. The aim of this study is to evaluate the possible protective role of curcumin on the histological and serologicalchanges of the hippocampus in diabetic rats.<br /> <strong><em>Materials and Methods:</em></strong> Forty albino rats were divided into four groups, ten rats each. Group 1 control rats, group 2 rats received curcumin orally (200 mg/kg/day for six weeks), group 3 rats were injected intraperitoneally with streptozotocin (STZ) (100 mg/kg, single dose), group 4 received a single injection of STZ and received curcumin orally for six weeks. Paraffin sections of hippocampus were prepared and stained with hematoxylin and eosin stain, and immnunohistochemical staining for GFAP and caspase-3. Morphometrical and statistical analyses were performed. Glycemic status and parameters of oxidative stress was measured.<br /> <strong><em>Results:</em></strong> Examination of hippocampus of diabetic rats showed disorganization of small pyramidal cells in CA1, many cellular losses in the pyramidal cells of CA3, many degenerated granule cells in the dentate gyrus. GFAP positive astrocyte and caspase-3 positive neuron counts were significantly increased. There were significant serum glucose elevation and significant lowered levels of oxidative stress parameters as compared to control rats. Curcumin administration improved the structural and serological alterationsof the hippocampuswith significant reduction in serum glucose level.<br /> <strong><em>Conclusion:</em></strong> Curcumin ameliorates the deterious effect of diabetes on the hippocampus through its antioxidant, antiapoptotic and anti-inflammatory efficacies.
Curcumin,Diabetes,Hippocampus,Neuroprotective,Rats
https://ijbms.mums.ac.ir/article_8839.html
https://ijbms.mums.ac.ir/article_8839_bad1139295f7f82d2c50bcc91c43ec70.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Restoration of correct splicing in IVSI-110 mutation of β-globin gene with antisense oligonucleotides: implications and applications in functional assay development
700
707
EN
Sima
Mansoori Derakhshan
Department of Medical Genetics, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
mansooris@tbzmed.ac.ir
Mahmoud
Shekari Khaniani
Department of Medical Genetics, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
shekari@yahoo.com
10.22038/ijbms.2017.8840
<strong><em>Objective(s)</em></strong>: The use of antisense oligonucleotides (AOs) to restore normal splicing by blocking the recognition of aberrant splice sites by the spliceosome represents an innovative means of potentially controlling certain inherited disorders affected by aberrant splicing. Selection of the appropriate target site is essential in the success of an AO therapy. In this study, in search for a splice model system to facilitate the evaluation of AOs to redirect defective splicing of IVSI-110 β-globin intron, an EGFP-based IVSI-110 specific cellular reporter assay system has been developed and a number of AOs were tested in this cellular splicing assay.<br /> <strong><em>Materials and Methods: </em></strong>A recombinant plasmid (pEGFP/I-110) carrying the EGFP gene interrupted by a mutated human β-globin intron 1 (IVSI-110) was developed and transfected into K562 cells. A number of AOs with a 2’-O-methyl oligoribonucleotide (2’-O-Me) backbone system were systematically tested in this cellular splicing assay.<br /> <strong><em>Results:</em></strong> The mutation in the intron causes aberrant splicing of EGFP pre-mRNA, preventing translation of EGFP; however, treatment of the cells with specific concentration of a sequence specific 2’-O-Me AO targeted to the aberrant splice site induced correct splicing and resulted in restoring of EGFP activity.<br /> <strong><em>Conclusion:</em></strong> This cellular splicing assay provides a novel functional assay system in assessing the cellular delivery efficiency of AOs and therapeutic effect of AOs in restoration of aberrant splicing.
Antisense,Beta-Thalassemia,Gene Therapy,Oligonucleotides,Splicing
https://ijbms.mums.ac.ir/article_8840.html
https://ijbms.mums.ac.ir/article_8840_26d164c378cb19921e5f7ce0f1d4c94c.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Repeated systemic administration of the cinnamon essential oil possesses anti-anxiety and anti-depressant activities in mice
708
714
EN
Reyhaneh
Sohrabi
Research Committee, Sabzevar University of Medical Sciences, Sabzevar, Iran
sohrabireyhaneh9212@gmail.com
Nasim
Pazgoohan
Research Committee, Sabzevar University of Medical Sciences, Sabzevar, Iran
nasim_pajohan@yahoo.com
Hasan
Rezaei Seresht
Traditional and Complementary Medicine Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
Bahareh
Amin
0000-0002-9121-7453
Cellular and Molecular Research Center, Department of Physiology and Pharmacology, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
amin.bahareh@gmail.com
10.22038/ijbms.2017.8841
<strong><em>Objective(s)</em></strong>: The present study aimed to evaluate the putative antidepressant and anti-anxiety effects of the cinnamon essential oil when administered acute (for 3 doses) and sub-acute (for 14 days) to mice.<br /> <strong><em>Materials and Methods: </em></strong>In an acute experimental study, forced swim test (FST) was conducted to evaluate the antidepressant-like behavior of animals treated with the intraperitoneal (IP) essential oil of cinnamon in triple doses (0.5, 1, and 2 mg/kg). In a sub-acute study (14 days in 24-hr intervals) antidepressant-like effects of essential oil (0.5, 1, and 2 mg/kg) with the same route were assessed in FST and tail suspension test (TST). Anti-anxiety and motor activities were evaluated using elevated plus-maze (EPM) and open field tests, respectively. Determination of different constituents within the sample oil was via gas chromatography–mass spectrometry (GC–MS) analysis.<br /> <strong><em>Results:</em></strong> Repetitive administration of cinnamon essential oil (0.5, 1, 2 mg/kg) during 14 days significantly decreased the time of immobility in both FST and TST as compared to the control group. Mice treated with oil at the dose of 2 mg/kg spent a longer time and had more entries into the open arms of EPM as compared with the vehicle-treated ones. According to GC-MS analysis, 46 chemical compounds were identified in the studied cinnamon essential oil with the main constituent being trans-cinnamaldehyde (87.32%).<br /> <strong><em>Conclusion:</em></strong> Cinnamon essential oil might be used as an adjunctive therapy in improving symptoms of depressive and anxiety disorders. However, dose-response effects need further evaluation. Trans-cinnamaldehyde might be responsible for the beneficial effect observed.
Cinnamon bark,Elevated plus maze Essential oil,Forced swimming test,Tail suspension test
https://ijbms.mums.ac.ir/article_8841.html
https://ijbms.mums.ac.ir/article_8841_4cdc51765f8b748d048f6c8731572d30.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Mori cortex prevents kidney damage through inhibiting expression of inflammatory factors in the glomerulus in streptozocin-induced diabetic rats
715
721
EN
Lili
Ma
Runliang Diabetes Laboratory, Diabetes Research Center, Ningbo University. Ningbo, Zhejiang, PR China
mllstyle@163.com
Hailai
Ni
Department of Prevention and Health, Changhai Hospital, Second Military Medical University, Shanghai, PR China
mzhnhl@aliyun.com
Xinrong
Zou
Runliang Diabetes Laboratory, Diabetes Research Center, Ningbo University. Ningbo, Zhejiang, PR China
zxrong0123@163.com
Yanyan
Yuan
Runliang Diabetes Laboratory, Diabetes Research Center, Ningbo University. Ningbo, Zhejiang, PR China
934194887@qq.com
Chun
Luo
Runliang Diabetes Laboratory, Diabetes Research Center, Ningbo University. Ningbo, Zhejiang, PR China
495388856@qq.com
Bingyang
Liu
Runliang Diabetes Laboratory, Diabetes Research Center, Ningbo University. Ningbo, Zhejiang, PR China
1018791864@qq.com
Fuyan
Wang
Runliang Diabetes Laboratory, Diabetes Research Center, Ningbo University. Ningbo, Zhejiang, PR China
wangfuyan@nbu.edu.cn
Yang
Xi
Runliang Diabetes Laboratory, Diabetes Research Center, Ningbo University. Ningbo, Zhejiang, PR China
xiyang@nbu.edu.cn
Yudong
Chu
Department of Nephrology, Ningbo Medical Center Lihuili Eastern Hospital, Ningbo, Zhejiang, PR China
chuyudong@foxmail.com
Pangjie
Xu
Department of Nephrology, Ningbo Medical Center Lihuili Eastern Hospital, Ningbo, Zhejiang, PR China
larkxu105@163.com
Xiaohui
Qiu
Department of Nephrology, Ningbo Medical Center Lihuili Eastern Hospital, Ningbo, Zhejiang, PR China
wolt2@163.com
Song
Li
Center for Pharmacogenetics, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15261, USA
lslengfeng@126.com
Shizhong
Bu
Runliang Diabetes Laboratory, Diabetes Research Center, Ningbo University. Ningbo, Zhejiang, PR China
bushizhong@nbu.edu.cn
10.22038/ijbms.2017.8842
<strong><em>Objective(s)</em></strong>: It has been widely reported that <em>Mori cortex extract</em> (MCE) is used for the treatment of diabetes mellitus in traditional medicine. The present study was designed to investigate its mechanism of action in the treatment of diabetic nephropathy (DN). We assessed whether MCE preventive treatment ameliorates kidney damage in high-fat diet and streptozotocin (STZ)-induced type 2 diabetic rats.<br /> <strong><em>Materials and Methods: </em></strong>Rats were fed a high-fat diet and injected with STZ. MCE was given to rats daily at 10 g/kg. Fasting blood glucose (FBG) and postprandial plasma glucose were measured. Blood and urine biochemical parameters, renal tissue morphology, and inflammation were investigated.<br /> <strong><em>Results: </em></strong>Prevention with MCE significantly decreased FBG and homoeostasis model assessment (HOMA) of IR (HOMA-IR) levels and increased insulin levels in diabetic rats. MCE prevention significantly decreased levels of KW/BW, BUN, Cr, and 24 hr urinary protein. MCE inhibited glomerular basement membrane thickening, tubular epithelial cell hypertrophy, and glomerular capillary dilation. MCE also prevented the disappearance of bowman’s space and renal tubular lumen and decreased collagen deposition in rat kidney. Moreover, MCE reduced the levels of inflammatory factors (MCP-1 and TNF-α) and fibrosis factors (collagen IV and fibronectin).<br /> <strong><em>Conclusion:</em></strong> MCE prevents DN through inhibition of inflammation and fibrosis in a rat model. It might provide a safe and effective way to prevent DN.
Diabetic nephropathy,Herb,Inflammation,Renal injury
https://ijbms.mums.ac.ir/article_8842.html
https://ijbms.mums.ac.ir/article_8842_164e17138a1ad27d8525f453660513d9.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
20
6
2017
06
01
Design of peptides interfering with iron-dependent regulator (IdeR) and evaluation of Mycobacterium tuberculosis growth inhibition
722
728
EN
Himen
Salimizand
Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
hsalimizand@gmail.com
Saeid
Amel Jamehdar
Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
ameljs@muma.ac.ir
Leila
Babaei Nik
Tuberculosis Reference Center, Dr Shariati Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
Hamid
Sadeghian
Antimicrobial Resistance Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
sadeghianh@mums.um.ac.ir
10.22038/ijbms.2017.8859
<strong><em>Objective(s)</em></strong>: Tuberculosis (TB), a disease caused by <em>Mycobacterium tuberculosis</em> (<em>Mtb</em>), stayed a global health thread with high mortality rate. Since TB has a long-term treatment, it leads high risk of drug resistant development, and there is an urgent to find new drugs. The aim of this study was designing new inhibitors for a new drug target, iron dependent regulator, IdeR.<br /> <strong><em>Materials and Methods: </em></strong>Based on the interaction most populated amino acids of IdeR to the related gene operators, 50 short peptides were modeled. Bonding affinity of short peptides toward DNA were studied by docking. Top 10 best predicted bonding affinity were selected. DNA binding assay, microplate alamar blue assay, colony counting, quantitative real time- PCR (qRT-PCR) of IdeR corresponding genes, cell wall-associated mycobactin and whole-cell iron estimation were done to prove the predicted mechanism of <em>in silico</em> potent constructs.<br /> <strong><em>Results:</em></strong> Amongst the 10 synthesized short peptide candidates, glycine-valine-proline-glycine (GVPG) and arginine-proline-arginine (RPR) inhibited <em>Mtb</em> <em>in vitro</em> at 200 µM concentration. qRT-PCR showed <em>mbtB</em> 58-fold over expression that resulted in <em>Mtb</em> growth inhibition. Cell wall-associated mycobactin and whole-cell iron estimation confirmed the results of qRT-PCR.<br /> <strong><em>Conclusion:</em></strong> We introduced two new lead compounds to inhibit <em>Mtb </em>that are promising for the development of more potent anti-tubercular therapies.
IdeR,Inhibitor,Modeling,Mycobacterium tuberculosis,RT-PCR
https://ijbms.mums.ac.ir/article_8859.html
https://ijbms.mums.ac.ir/article_8859_59adfaadea14834061f0c7b56054e00f.pdf