Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
16
5
2013
05
01
Publication Ethics: Many Facets, Collaboration Required
664
664
EN
Viroj
Wiwanitkit
Visiting Professor, Hainan Medical University, China; Adjunct Professor, Joseph Ayobabalola University, Nigeria
2 Visiting Professor, Faculty of Medicine, University of Nis, Serbia
wviroj@pioneer.netserv.chula.ac.th
10.22038/ijbms.2013.790
Sir, the recent publication on “Publication Ethics” is very interesting (1). The article by Fazly Bazzaz and Sadeghi demonstrated many interesting cases of misconducts. Indeed, the misconducts can be seen in many ways. As mentioned in the present publication, both author and editor can perform publication misconducts. Nevertheless, the problem has many more facets. Sometimes, the misconducts are generated by the third parties. For example, the publisher can perform publication misconduct. In fact, the editor takes the role in control of the materials to be published in the journal. However, the publisher sometimes interferes with the process. Some unethical publication can be seen in some “predator” online publisher. “Pay then publish” without control of its quality can be seen (see also http://www.nature.com/news/predatory-publishers-are-corrupting-open-access-1.11385 and scholarlyoa.com/2012/12/06/bealls-list-of-edatory-publishers-2013). In the present era of rapidly increased number of open access journal, this problem is expected to increase. Another example is the criminal case of “one disguising to be the other person” to perform misconduct aiming at discrediting or destroying others. An interesting case was previously published in Hepatitis Monthly; an author performed plagiarized articles behind the name of another person <br/>and finally was charged by the IT investigation (2). Finally, the institute sometimes also performs misconduct. This can be supporting of their members who perform scientific misconduct by acts which do not correspond to the problem (this problem is usually seen in the problematic case generated by senior faculties or administrators), dealing with publisher to “buy” supplementary volume for publication of the works from the institute with low standard of peer <br/>reviewing process, citing the name of some famous scientists into the publication of the institute without previous asking for permission, etc. In order to cope <br/> <br/>with those problems, the collaboration between all partners (author, editor, journal, institute, reviewer and reader) is needed. Sharing of knowledge, learning together and going together toward the ethical publication is recommended.
https://ijbms.mums.ac.ir/article_790.html
https://ijbms.mums.ac.ir/article_790_de071a3b9f102708e72568e09b15bfee.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
16
5
2013
05
01
The Effect of Maternal Thyroid Disorders (Hypothyroidism and Hyperthyroidism) During Pregnancy and Lactation on Skin Development in Wistar Rat Newborns
665
674
EN
Maryam
Amerion
Azad University of Shahrud, Medical Sciences School, Shahrud, Iran
Somayye
Tahajjodi
Ferdowsi University of Mashhad, Faculty of Basic Sciences, Mashhad, Iran
Zahra
Hushmand
Ferdowsi University of Mashhad, Faculty of Basic Sciences, Mashhad, Iran
Nasser
Mahdavi Shahri2
Ferdowsi University of Mashhad, Faculty of Basic Sciences, Mashhad, Iran
Mohammad Reza
Nikravesh
Mashhad University of Medical Sciences school, Mashhad, Iran
nikravesh@hotmail.com
Mahdi
Jalali
Mashhad University of Medical Sciences school, Mashhad, Iran
jalalim@mums.ac.ir
10.22038/ijbms.2013.788
<br/><br/><em><span style="font-size: xx-small;">Objective(s): </span></em><br/><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;">Previous studies have shown that thyroid hormones are necessary for normal development of many organs and because of the importance of skin as the largest and the most important organ in human body protection in spite of external environment, the study of thyroid hormones effects on skin development is considerable. In this survey we have tried to study the effects of maternal hypothyroidism on skin development in fetus during pregnancy and lactation by immunohistochemistry technique. </span><br/> <br/>Materials and Methods: <br/><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;"><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;">Rats were divided into 4 groups, hypothyroids, hyperthyroids, hypothyroids are treated with levothyroxin and a control group. The rat mothers were exposed to PTU with 50 mg/lit dosage and levothyroxin with 1 mg/lit dosage and PTU and levothyroxin simultaneously and with the same dosage respectively in hypothyroid, hyperthyroid and treated hypothyroids with levothyroxin groups. After 14 days, blood sample was taken from mothers, and if thyroid hormones level had change well, mating was allowed. After pregnancy and delivery, 1th day dorsal skin (as the sample for pregnancy assay) and 10th day skin (as for lactation assay) was used for immunohystochemical and morphometric studies. </span></span><br/>Results: <br/><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;"><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;">In this study it was observed that maternal hypothyroidism during pregnancy and lactation causes significant increase in laminin expression, in most areas of skin, and maternal hyperthyroidism during pregnancy and lactation causes significant decrease in laminin expression. Also significant decrease was observed in hair follicles number and epidermis thickness in hypothyroidism groups. </span></span><br/>Conclusion: <br/><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;"><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;">This study showed maternal hypothyroidism causes significant decrease in epidermis thickness and hair follicles number and it causes less hair in fetus. Also maternal hypothyroidism causes large changes in laminin expression in different parts of skin. At the same time,maternal hyperthyroidism causes opposite results. In fact, thyroid hormones regulate laminin expression negatively which means increase in thyroid hormone level, decreases laminin expression. So changes in thyroid hormones level can influence skin development significantly. </span></span>
Keywords:
Hypothyroidism
Lactation
Laminin
Maternal
Pregnancy
Skin development
https://ijbms.mums.ac.ir/article_788.html
https://ijbms.mums.ac.ir/article_788_4e6621a033aa1d1ec3f987e328a7e0db.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
16
5
2013
05
01
Identification of Novel Hypoxia Response Genes in Human Glioma Cell Line A172
675
682
EN
Fatemeh
Baghbani
1Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Reza
Raoofian
1Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
rraoofian@yahoo.com
Mohammad
Hasanzadeh Nazarabadi
1Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Tayebeh
Hamzehloei
1Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad
Soukhtanloo
Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mansur
Heidari
of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Seyed Morteza
Afsharzadeh
1Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Sahar
Shekouhi
1Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Fahimeh
Moradi
1Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Ira
Abdol-Azim
Sarli
1Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Javad
Zavar-Reza
Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Majid
Mojarrad
1Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
10.22038/ijbms.2013.779
<br/><br/><em><span style="font-size: xx-small;">Objective(s): </span></em><br/><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;">Hypoxia is a serious challenge for treatment of solid tumors. This condition has been manifested to exert significant therapeutic effects on glioblastoma multiform or (WHO) astrocytoma grade IV. Hypoxia contributes numerous changes in cellular mechanisms such as angiogenesis, metastasis and apoptosis evasion. Furthermore, in molecular level, hypoxia can cause induction of DNA breaks in tumor cells. Identification of mechanisms responsible for these effects can lead to designing more efficient therapeutic strategies against tumor progression which results in improvement of patient prognosis. </span><br/> <br/>Materials and Methods: <br/><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;"><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;">In order to identify more hypoxia regulated genes which may have a role in glioblastoma progression, cDNA-AFLP was optimized as a Differential display method which is able to identify and isolate transcripts with no prior sequence knowledge. </span></span><br/>Results: <br/><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;"><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;">Using this method, the current study identified 120 Transcription Derived Fragments (TDFs) which were completely differentially regulated in response to hypoxia. By sequence homology searching, the current study could detect 22 completely differentially regulated known genes and two unknown sequence matching with two chromosome contig and four sequence matches with some Expressed Sequence Tags (ESTs). </span></span><br/>Conclusion: <br/><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;"><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;">Further characterizing of these genes may help to achieve better understanding of hypoxia mediated phenotype change in tumor cells. </span></span>
cDNA-AFLP
Glioblastoma
Hypoxia
https://ijbms.mums.ac.ir/article_779.html
https://ijbms.mums.ac.ir/article_779_382a724d6d0818136d00202e8005fb22.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
16
5
2013
05
01
The Effects of a Moderate Exercise Program on Knee Osteoarthritis in Male Wistar Rats
683
688
EN
Mohammad
Fallah Mohammadi
Department of Sport Pathology and Corrective Exercises, School of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran
mohammad1671@yahoo.com
Akbar
Hajizadeh Moghaddam
Department of Biology, Faculty of Sciences, University of Mazandaran, Babolsar, Iran
Hosein
Mirkarimpur
Department of Sport Pathology and Corrective Exercises, School of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
10.22038/ijbms.2013.780
<br/><br/><em><span style="font-size: xx-small;">Objective(s): </span></em><br/><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;">Osteoarthritis (OA) or degenerative joint disease is the commonest form of arthritis and can lead to joint pain, decrease in joint’s range of motion, loss of function, and ultimately disability. Exercise is considered as one of the non-pharmacological treatments of OA. But the effects of exercise on knee joint cartilage remain ambiguous. The aim of the present study was to investigate the effect of a four-week moderate treadmill exercise on rats’ knee osteoarthritis. </span><br/> <br/>Materials and Methods: <br/><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;"><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;">Eighteen male Wistar rats (173 ± 1 g, 8 weeks old) were randomly divided into three groups (n = 6): Intact control, monosodium iodoacetate (MIA) only (OA), and training. The osteoarthritis model was induced by intra-articular injection of monosodium iodoacetate (MIA). Subjects followed a moderate-intensity exercise program for 28 days. Rats were killed after 28 days and histological assessment was done on their knee joints. One-way ANOVA (P<0.05) and post-hoc Tukey test was used for the statistical analysis. </span></span><br/>Results: <br/><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;"><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;">Histological assessment on 3 measurements of, depth ratio of lesions (P=0.001), total cartilage degeneration width (P=0.001), and significant cartilage degeneration width (P=0.001), demonstrated that moderate exercise for 4 weeks could surprisingly almost treat OA symptoms of rats’ knee joints. </span></span><br/>Conclusion: <br/><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;"><span style="font-family: Cambria Math,Cambria Math; font-size: xx-small;">The findings of the present study indicate that a moderate treadmill exercise program exert a beneficial influence on rats’ knee osteoarthritis. </span></span>
Exercise
Monosodium Iodoacetate
Rat’s Knee Osteoarthritis
https://ijbms.mums.ac.ir/article_780.html
https://ijbms.mums.ac.ir/article_780_bbe2914c984e6b581f3b033fcb42fc35.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
16
5
2013
05
01
A Comparative Study of Serum Level of Vascular Cell Adhesion Molecule-1 (sVCAM-1), Intercellular Adhesion Molecule-1(ICAM-1) and High Sensitive C - reactive protein (hs-CRP) in Normal and Pre-eclamptic Pregnancies
689
693
EN
Mehdi
Farzadnia
Department of Pathology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran,3Cancer Molecular Pathology Research Centre, Mashhad University of Medical Sciences. Mashhad, Iran
Hossein
Ayatollahi
Departments of Hematopathology and Blood Banking; Faculty of Medicine, Mashhad University of Medical Sciences. Mashhad, Iran
3Cancer Molecular Pathology Research Centre, Mashhad University of Medical Sciences. Mashhad, Iran
Maliheh
Hasan-zade
Department of Obstetrics and Gynecology, Faculty of Medicine, Mashhad University of Medical Sciences. Mashhad, Iran
Hamid Reza
Rahimi
Student Research Committee, Department of Modern Sciences & Technologies, Faculty of Medicine, Mashhad University of Medical Sciences. Mashhad, Iran
10.22038/ijbms.2013.781
Adhesion Molecule-1 (sVCAM-1)
High sensitive C-reactive protein (hs-CRP)
Intercellular adhesion molecule-1(ICAM-1)
Pre-eclampsia
Soluble vascular cell
https://ijbms.mums.ac.ir/article_781.html
https://ijbms.mums.ac.ir/article_781_645dc1dcc35edff143518a5dc58469a2.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
16
5
2013
05
01
The Effect of Opium Dependency of Parent (s) on Offspring’s Spatial Learning & Memory in Adult Male Rats
694
699
EN
Arezoo
Saberi Moghadam
Kerman Neuroscience Research Center, Kerman University of Medical Sciences Kerman, Iran
Gholamreza
Sepehri
Kerman Neuroscience Research Center, Kerman University of Medical Sciences Kerman, Iran
Vahid
Sheibani
Kerman Neuroscience Research Center, Kerman University of Medical Sciences Kerman, Iran
vsheibani2@yahoo.com
Tahereh
Haghpanah
Department of Anatomical and Reproductive Biology, Faculty of Medical sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Kouros
Divsalar
Kerman Neuroscience Research Center, Kerman University of Medical Sciences Kerman, Iran
Mousa-Al-Reza
Hajzadeh
Mashhad Cognitive Neuroscience Research Center and Department of Physiology, Faculty ofMedical sciences, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammadreza
Afarineshkhaki
Department of Physiology and Medical Student Research Committee, Faculty of Medical sciences, Shahid Beheshti University of Medical Sciences. Tehran, Iran
10.22038/ijbms.2013.782
<em>Objective(s):</em>As far as we know,there has been no report regarding the effects of opium addiction or dependency of both parents on the learning and memory process in offspring. The aim of this study was to examine the learning and memory changes of adult male offspring whose mothers, fathers and/or both parents had dependency to opium before and during pregnancy. <br/><em>Materials and Methods</em><em>:</em> All experiments were carried out on Wistar rats. Opium dependency was induced by daily injections of opium (10 mg/kg/SC, bid/10 d) before mating. The presence of a vaginal plug was designated as gestation day. Treatment with opium continued through breeding and gestation until parturition. Spatial memory was tested in male offspring of control, saline and prenatal opium treated groups by a training trial and the probe test in the Morris water maze. Swimming escape latency in the maze and the ability to find the platform in the training trial were recorded. The time spent in the trigger zone and number of times the rats crossed the platform during the probe phase and swimming speed were measured. <br/><em>Results:</em>Thedata revealed increased escape latency and a greater distance traveled to find the hidden platform in the offspring’s whose mother, father and /or both parents were exposed to opium. Crossings to target quadrant at probe trials was significantly reduced in all of the prenatal opium exposed offsprings. The swimming speed showed a significant increase in father and parent’s opium exposed offspring. <br/><em>Conclusion:</em>Prenatal opium exposure of either parent may cause deficits in spatial learning, but the precise mechanism(s) remain largely unknown.
Learning
Memory
Opium
Parent (s) dependency
Rat
https://ijbms.mums.ac.ir/article_782.html
https://ijbms.mums.ac.ir/article_782_942f2e385927e7f727cb9a09bf889578.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
16
5
2013
05
01
Role of SOX9 in the Etiology of Pierre-Robin Syndrome
700
704
EN
Selvi
R
Department of Human Genetics, Sri Ramachandra University, Porur, Chennai-116, Tamil Nadu, India
Mukunda Priyanka
A
Department of Human Genetics, Sri Ramachandra University, Porur, Chennai-116, Tamil Nadu, India
10.22038/ijbms.2013.783
<em>Objective(s</em><em>:</em>Cleft lip/palate are common congenital anomalies, affecting approximately 2/1000 live births. Pierre Robin Sequence is a subgroup of the cleft palate population. Chromosomal abnormalities near the SOX9 gene disrupt the regulation of this gene and prevent the SOX9 protein from properly controlling the development of facial structures, which leads to isolated PRS. The present study was conducted to identify the role of the <em>SOX9</em> gene in the etiology of Pierre robin syndrome and to study the association of <em>SOX9</em> and PRS in regulating morphogenesis of the face in individuals with Cleft lip/Palate using the PCR technique and GTG banding. <br/><em>Materials and Methods:</em>Molecular and cytogenetic analysis was performed in 27 subjects with cleft lip/palate and 13 age matched controls. DNA was isolated and PCR was performed for the amplification of the gene of interest and the products were run on a 2% Agarose gel and the band patterns were analyzed.The chromosomal abnormalities were analyzed from the cultured lymphocytes after GTG banding. <br/><em>Result:</em>Out of 27 patients screened, deletion of the <em>SOX9</em> gene was observed in 1 case for exon1 and in 2 cases for exon2. The cytogenetic analysis showed no structural or numerical abnormalities and all the patients showed normal karyotype. <br/><em>Conclusion:</em> The results of molecular methods showed a positive association suggesting that the SOX9 gene is of particular importance, but the cytogenetic study didn’t seem to show a stronger association suggesting that, this method would not identify disease genes acting via other mechanisms of genetic dominance and also due to the fact that Cleft lip / palate has a multifactorial inheritance.
Cleft lip & Palate
Cytogenetics
PCR
Pierre Robin Syndrome SOX9
https://ijbms.mums.ac.ir/article_783.html
https://ijbms.mums.ac.ir/article_783_4ddfc95ffa7ca16ce12cac227724c3d3.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
16
5
2013
05
01
Development of 177Lu-phytate Complex for Radiosynovectomy
705
709
EN
Hassan
Yousefnia
Radiopharmaceutical Research and Development Lab, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran, P.O.Box:14395-836
2 Faculty of Energy Engineering and Physics, Amirkabir University of Technology. Tehran, Iran
Amir Reza
Jalilian
Radiopharmaceutical Research and Development Lab, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran, P.O.Box:14395-836
Ali
Bahrami-Samani
Radiopharmaceutical Research and Development Lab, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran, P.O.Box:14395-836
Mohammad
Mazidi
Radiopharmaceutical Research and Development Lab, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran, P.O.Box:14395-836
Mohammad
Ghannadi Maragheh1
Radiopharmaceutical Research and Development Lab, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran, P.O.Box:14395-836
Fereydoun
Abbasi-Davani
Radiation Application Group, Faculty of Nuclear Engineering, Shahid Beheshti University, Tehran, Iran
10.22038/ijbms.2013.784
<em>Objective(s):</em> In this work a new possible agent for radiosynovectomy has been targeted for articular pain palliation. <br/><em>Materials and Methods:</em> Lu-177 of 2.6-3 GBq/mg specific activity was obtained by irradiation of natural Lu<sub>2</sub>O<sub>3 </sub>sample with thermal neutron flux of 4 × 10<sup>13</sup> n.cm<sup>-2</sup>.s<sup>-1</sup>. The product was converted into chloride form which was further used for labeling of <sup>177</sup>Lu-phytate complex and checked using ITLC (MeOH: H<sub>2</sub>O: acetic acid, 4: 4: 2, as mobile phase). The complex stability and viscosity were checked in the final solution up to seven days. The prepared complex solution (100 µCi/100 µl) was injected intra-articularly to male rat knee joint. Leakage of radioactivity from injection site and its distribution in organs were investigated up to seven days. <br/><em>Results:</em> The complex was successfully prepared with high radiochemical purity (>99.9 %). Approximately, the whole injected dose has remained in injection site seven days after injection. <br/><em>Conclusion: </em>The complex was proved to be a feasible agent for cavital radiotherapy in oncology and rheumatology
Biodistribution
Lutetium-177
Phytate Radiosynovectomy
https://ijbms.mums.ac.ir/article_784.html
https://ijbms.mums.ac.ir/article_784_6eec0c8aca6a827fc28fe7a9048a4042.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
16
5
2013
05
01
Synergistic Effect of Subtoxic-dose Cisplatin and TRAIL to Mediate Apoptosis by Down-regulating Decoy Receptor 2 and Up-regulating Caspase-8, Caspase-9 and Bax Expression on NCI-H460 and A549 Cells
710
718
EN
Xiaoyan
Zhang
1Department of Medical Oncology, Cancer Center of West China Hospital, Sichuan University, Chengdu, Sichuan Province, China
Jing
Zhao
Department of Oncology, Hebei General Hospital, Shijiazhuang, Hebei Province, China
Wenyan
Zhu
1Department of Medical Oncology, Cancer Center of West China Hospital, Sichuan University, Chengdu, Sichuan Province, China
Hongfeng
Gou
1Department of Medical Oncology, Cancer Center of West China Hospital, Sichuan University, Chengdu, Sichuan Province, China
Dan
Cao
1Department of Medical Oncology, Cancer Center of West China Hospital, Sichuan University, Chengdu, Sichuan Province, China
Yu
Yang
1Department of Medical Oncology, Cancer Center of West China Hospital, Sichuan University, Chengdu, Sichuan Province, China
Ying
Huang
2Department of Pathophysiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province, China
Cheng
Yi
1Department of Medical Oncology, Cancer Center of West China Hospital, Sichuan University, Chengdu, Sichuan Province, China
huaxiwxh_siru@yahoo.com.cn
10.22038/ijbms.2013.785
<em>Objective(s)</em><em>:</em> Although tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can selectively induce apoptosis in tumor cells, more than half of tumors including non-small cell lung cancer (NSCLC) exhibit TRAIL-resistance. The purpose of this study was to determine whether subtoxic-dose cisplatin and TRAIL could synergistically enhance apoptosis on NSCLC cells and investigate its underlying mechanisms. <br/><em>Materials and Methods:</em>NCI-H460 and A549 cells were treated with TRAIL alone, cisplatin alone or combination treatment in this study. The cytotoxicity was evaluated according to Sulforhodamine B assay, and apoptosis was examined using Hoechst 33342 staining and flow cytometry. The mRNA and protein levels of TRAIL receptors and apoptotic proteins including caspase-8, caspase-9, Bcl-2 and Bax were determined by RT-PCR and Western blotting, respectively. <br/><em>Results:</em>Our results showed that NCI-H460 cells were sensitive to TRAIL, whereas A549 cells were resistant. However, subtoxic-dose cisplatin could enhance the both cells to TRAIL-mediated cell proliferation inhibition and apoptosis. The underlying mechanisms might be associated with the down-regulation of DcR2 and up-regulation of Caspase-8, Caspase-9 and Bax. <br/><em>Conclusion:</em>Subtoxic-dose cisplatin could enhance both TRAIL- sensitive and TRAIL- resistant NSCLC cells to TRAIL-mediated apoptosis. These findings motivated further studies to evaluate such a combinatory therapeutic strategy against NSCLC in the animal models.
Apoptosis
Cisplatin
DcR2
Nonsmall cell lung cancer
Subtoxic-dose
TRAIL
https://ijbms.mums.ac.ir/article_785.html
https://ijbms.mums.ac.ir/article_785_c83ff34a4cca81b1affd5d1a0007de9a.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
16
5
2013
05
01
Production, Quality Control and Biological Evaluation of 166Ho-PDTMP as a Possible Bone Palliation Agent
719
725
EN
Samaneh
Zolghadri
Radiopharmaceutical Research & Development Laboratory (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran
2 Faculty of Energy Engineering and Physics, Amirkabir University of Technology, Tehran, Iran
Amir Reza
Jalilian
Radiopharmaceutical Research & Development Laboratory (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran
2 Faculty
Zohreh
Naseri
Radiopharmaceutical Research & Development Laboratory (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran
Hassan
Yousefnia
Radiopharmaceutical Research & Development Laboratory (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran
Ali
Bahrami-Samani
Radiopharmaceutical Research & Development Laboratory (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran
Mohammad
Ghannadi-Maragheh
Radiopharmaceutical Research & Development Laboratory (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran
Hossein
Afarideh
Faculty of Energy Engineering and Physics, Amirkabir University of Technology, Tehran, Iran
10.22038/ijbms.2013.786
<em>Objective(s):</em>In this study, <sup>166</sup>Ho-1,2-propylene di-amino tetra(methy1enephosphonicAcid) (<sup>166</sup>Ho-PDTMP) complex was prepared as a bone palliation agent. <br/><em>Materials and Methods:</em>The complex was successfully prepared using an in-house synthesized EDTMP ligand and <sup>166</sup>HoCl<sub>3</sub>. Ho-166 chloride was obtained by thermal neutron irradiation (1 × 1013 n.cm-2.s-1) of natural Ho(NO<sub>3</sub>)<sub>3</sub> samples followed by radiolabeling and stability studies. Biodistribution in wild type rats was also peformed. <br/><em>Results: </em>The complex was prepared with thespecific activity of 278 GBq/mg and high radiochemical purity (>99%, checked by ITLC). <sup>166</sup>Ho-PDTMP complex was stabilized in the final preparation and in the presence of human serum (>90%) up to 72 hr. The biodistribution of <sup>166</sup>Ho-PDTMP in wild-type rats demonstrated significant bone uptake was up to 48 hr compared to <sup>166</sup>HoCl<sub>3</sub>. <br/><em>Conclusion:</em> The produced <sup>166</sup>Ho-PDTMP properties suggest a possible new bone palliative therapeutic to overcome the metastatic bone pains. <br/>
Biodistribution
166Ho
PDTMP
Radiopharmaceutical
Therapy
https://ijbms.mums.ac.ir/article_786.html
https://ijbms.mums.ac.ir/article_786_0de4dbae39e7b3035979ad4f770e83f7.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
16
5
2013
05
01
The Effect of Celecoxib, a Cyclooxygenase-2 Inhibitor on Noise- Induced Hearing Loss
726
730
EN
Akram
Pourbakht
Department of Audiology, Faculty of Rehabilitation, Rehabilitation Research Center, Iran University of Medical Sciences, Tehran, Iran
a-pourbakht@tums.ac.ir
10.22038/ijbms.2013.787
<em>Objective(s): </em>Noise-induced hearing loss (NIHL) is the major cause of acquired hearing loss. Celecoxib, a cyclooxygenase-2 (COX-2) inhibitor, is a non- steroidal anti- inflammatory drug (NSAID) with known antioxidant and antineoplastic activity. Therefore, we monitored the extent of temporary noise- induced threshold shifts (TTS) and cochlear damage caused by high level 4- kHz noise exposure to verify the differences with those pretreated with celecoxib. <br/><em>Materials and Methods:</em> Ten male albino guinea pigs (300-350 g in weight) were randomly allocated into two groups: the primal group was exposed to 4- kHz octave band noise at 102 dB SPL for 3 hrs (group 1, n=5); the latter pretreated with 50 mg/ kg celecoxib for 3 days, then exposed to noise (group 2, n=5). Before exposure and one hr after noise exposure, threshold shifts were evaluated with auditory brainstem responses (ABR) and finally the animals were euthanized for histological evaluation. <br/><em>Results: </em>Comparing the threshold shifts before/after noise exposure with those pretreated, we found out that TTS caused by noise exposure did not show significant mitigation by celecoxib. By observing the organ of Corti at lower middle turn of cochlea in celecoxib pretreated group, considerable hair cell loss was discovered. <br/><em>Conclusion:</em>The current study clearly confirmed that celecoxib had no attenuation against temporary noise-induced hearing loss.
Auditory brainstem responses
Celecoxib
Guinea pigs
Hair cell
Threshold shift
Noise-induced hearing loss
https://ijbms.mums.ac.ir/article_787.html
https://ijbms.mums.ac.ir/article_787_50e25ccccaa9bd7b51c26d200afa11c3.pdf