TY - JOUR ID - 10031 TI - Evaluation of Tnf-α and Il-6 mRNAs expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrol JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Ghowsi, Mahnaz AU - Khazali, Homayoun AU - Sisakhtnezhad, Sajjad AD - Department of Physiology, Faculty of Biological Sciences and Technology, Shahid Beheshti University, Tehran, Iran AD - Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran Y1 - 2018 PY - 2018 VL - 21 IS - 2 SP - 165 EP - 174 KW - Adipose tissue KW - IL-6 KW - Polycystic ovary – KW - syndrome KW - Rat KW - Resveratrol KW - TNF-α DO - 10.22038/ijbms.2017.24801.6167 N2 - Objective(s): Some studies suggest that chronic low-grade inflammation is involved in insulin resistance in polycystic ovary syndrome (PCOS). This study assessed possible involvement of alteration in expression of two pro-inflammatory factors, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in adipose tissues of PCOS rats in the impairment of insulin actions. Also, effects of resveratrol as an anti-inflammatory agent were investigated. Materials and Methods: Fifteen female Wistar rats (21 days old) were divided into three groups (n=5): Ι) Control, П) PCO-model-saline: served as PCOS rats and to induce PCOS, received subcutaneously testosterone enanthate 1 mg/100 g body weight subcutaneously for 35 days, Ш) PCO-model-resveratrol, after receiving testosterone, received resveratrol 10 mg/kg intraperitoneally for 28 days. The expression of Tnf-α and Il-6 mRNAs in adipose tissues was determined by the qRT-PCR method. Results: The Il-6 mRNA expression in the visceral adipose tissue of PCOS rats was increased in comparison to controls (P<0.05). Tnf-α and Il-6 mRNA expression in visceral and subcutaneous adipose tissues of polycystic ovarian rats was similar to controls. The expression of Tnf-α mRNA in subcutaneous adipose tissue and Tnf-α and Il-6 mRNAs in the visceral adipose tissue of the PCO-model-resveratrol group were lower than PCOS rats (P<0.05). Conclusion: Increased expression of Il-6 mRNA in the visceral adipose tissue of polycystic ovarian rats may be one cause of insulin resistance observed in them and resveratrol as an anti-inflammatory and anti-hyperglycemic agent may decrease the risk of diabetes by reduction of expression of pro-inflammatory cytokines TNF-α and IL-6 in PCOS patients. UR - https://ijbms.mums.ac.ir/article_10031.html L1 - https://ijbms.mums.ac.ir/article_10031_eacda6285bacb04d43afe2538fdd614d.pdf ER -