TY - JOUR ID - 10466 TI - he effects of vitamin B12 on the brain damages caused by methamphetamine in mice JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Moshiri, Mohamad AU - Hosseiniyan, Seyed Mojtaba AU - Moallem, Seyed Adel AU - Hadizadeh, Farzin AU - Jafarian, Amir Hosein AU - Ghadiri, Ameneh AU - Hoseini, Toktam AU - Seifi, Mahmoud AU - etemad, leila AD - Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran AD - Department of Toxicology, Faculty of Pharmacy, Islamic Azad University, Shahreza Branch, Shahreza, Iran AD - Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran AD - Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran AD - Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran AD - Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran Y1 - 2018 PY - 2018 VL - 21 IS - 4 SP - 434 EP - 438 KW - Cerebral cortex Methamphetamine Neurotoxicity KW - Striatum KW - Vitamin B12 DO - 10.22038/ijbms.2018.23362.5897 N2 - Objective(s): Methamphetamine (METH) is a powerful stimulant drug that directly affects the brain and induces neurological deficits. B12 is a water-soluble vitamin (vit) that is reported to attenuate neuronal degeneration. The goal of the present study is to investigate the effect of vitamin B12 on METH’s neurodegenerative changes.Materials and Methods: Two groups of 6 animals received METH (10 mg/kg, interaperitoneally (IP)) four times with a 2 hr interval. Thirty mins before METH administration, vit B12 (1 mg/kg) or normal saline were injected IP. Animals were sacrificed 3 days after the last administration. Caspase proteins levels were measured by Western blotting. Also, samples were examined by TUNEL assay to detect the presence of DNA fragmentation. Reduced glutathione (GSH) was also determined by the Ellman method.Results: The pathological findings showed that vit B12 attenuates the gliosis induced by METH. Vit B12 administration also significantly decreased the apoptotic index in the striatum and the cerebral cortex (P<0.001). It also reduced caspase markers compared to the control (PConclusion: The current study suggests that parenteral vit B12 at safe doses may be a promising treatment for METH-induced brain damage via inhibition of  neuron apoptosis and increasing the reduced GSH level. Research focusing on the mechanisms involved in the protective responses of vit B12 can be helpful in providing a novel therapeutic agent against METH-induced neurotoxicity. UR - https://ijbms.mums.ac.ir/article_10466.html L1 - https://ijbms.mums.ac.ir/article_10466_e744fab65c3b61d699088d693dae0921.pdf ER -