TY - JOUR ID - 10542 TI - Insulin glargine affects the expression of Igf-1r, Insr, and Igf-1 genes in colon and liver of diabetic rats JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Juárez-Vázquez, Clara I AU - Gurrola-Díaz, Carmen M AU - Vargas-Guerrero, Belinda AU - Domínguez-Rosales, José A AU - Rodriguez-Ortiz, Jessica Fabiola AU - Barros-Núñez, Patricio AU - Flores-Martínez, Silvia E AU - Sánchez-Corona, José AU - Rosales-Reynoso, Mónica A AD - División de Medicina Molecular, Centro de Investigación Biomédica de Occidente. Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, México AD - Instituto de Enfermedades Crónico Degenerativas, Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara. Guadalajara, Jalisco, México AD - División de Genética, Centro de Investigación Biomédica de Occidente. Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, México. AD - División de Genética, Centro de Investigación Biomédica de Occidente. Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, México Y1 - 2018 PY - 2018 VL - 21 IS - 5 SP - 489 EP - 494 KW - Colon KW - Diabetes KW - Insulin glargine KW - Liver KW - Metformin KW - NPH insulin KW - Rats DO - 10.22038/ijbms.2018.24867.6185 N2 - Objective(s): The mitogenic effect of the analogous insulin glargine is currently under debate since several clinical studies have raised the possibility that insulin glargine treatment has a carcinogenic potential in different tissues. This study aimed to evaluate the Igf-1r, Insr, and Igf-1 gene expression in colon and liver of streptozotocin-induced diabetic rats in response to insulin glargine, neutral protamine Hagedorn (NPH) insulin, and metformin treatments. Materials and Methods: Male Wistar rats were induced during one week with streptozotocin to develop Type 2 Diabetes (T2D) and then randomly distributed into four groups. T2D rats included in the first group received insulin glargine, the second group received NPH insulin, the third group received metformin; finally, untreated T2D rats were included as the control group. All groups were treated for seven days; after the treatment, tissue samples of liver and colon were obtained. Quantitative PCR (qPCR) was performed to analyze the Igf-1r, Insr and Igf-1 gene expression in each tissue sample. Results: The liver tissue showed overexpression of the Insr and Igf-1r genes (P>0.001) in rats treated with insulin glargine in comparison with the control group. Similar results were observed for the Insr gene (P>0.011) in colonic tissue of rats treated with insulin glargine. Conclusion: These observations demonstrate that insulin glargine promote an excess of insulin and IGF-1 receptors in STZ-induced diabetic rats, which could overstimulate the mitogenic signaling pathways. UR - https://ijbms.mums.ac.ir/article_10542.html L1 - https://ijbms.mums.ac.ir/article_10542_7e264878e6e9c46dab650b5d7c6bc9c3.pdf ER -