TY - JOUR ID - 1116 TI - Gene Expression Status and Methylation Pattern in Promoter of P15INK4b and P16INK4a in Cord Blood CD34+ Stem Cells JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Azad, Mehdi AU - Kaviani, Saeid AU - Noruzinia, Mehrdad AU - Mortazavi, Yousef AU - Mobarra, Naser AU - Alizadeh, Shaban AU - Shahjahani, Mohammad AU - Skandari, Fatemeh AU - Ahmadi, Mohammad Hosein AU - Atashi, Amir AU - Abroun, Saeid AU - Zonoubi, Zahra AD - 1Hematology Department, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran AD - Hematology Department, Zanjan Medical Sciences University, Zanjan, Iran AD - Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 7Students’ Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran AD - 4Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran AD - Iranian Blood Transfusion Organizations, Medical Departmen AD - 6Department of Obstetrics and Gynecology, Mahdiyeh Hospital, Shahid Beheshti University,Tehran, Iran Y1 - 2013 PY - 2013 VL - 16 IS - 7 SP - 822 EP - 828 KW - Keywords: Gene expression Hematopoietic stem cells Methylation Tumor suppressor genes DO - 10.22038/ijbms.2013.1116 N2 -   Objective(s): Stem cell differentiation into different cell lineages depends upon several factors, cell cycle control elements and intracellular signaling elements, including P15INK4b and P16INK4a genes. Epigenetics may be regarded as a control mechanism which is affected by these factors with respect to their promoter structure.   Materials and Methods: The CD34 + cord blood stem cells were purified, isolated and then expanded. The undifferentiated day genome was isolated from part of the cultured cells, and the seventh day differentiated genome was isolated from the other part after differentiation to erythroid lineage. The procedure was followed by a separate Real-Time PCR for the two genes using the obtained cDNA. The processed DNA of the former stages was used for MSP (Methylation Specific PCR) reaction. Finally, pre- and post differentiation results were compared. Results: After performing MSP for each gene, it became clear that P15INK4b gene has undergone methylation and expression in predifferentiation stage. In addition, its status has not been changed after differentiation. P15INK4b gene expression was reduced after the differentiation. The other gene, P16INK4a, showed no predifferentiation methylation. Itwas completely expressed methylated and underwent reduced expression after differentiation. Conclusion : Specific predifferentiation expression of P15INK4b and P16INK4a genes along with reduction in their expression after erythroid differentiation indicated animportant role for these two genes in biology of CD34+ cells in primary stages and before differentiation. In addition, both genes are capable of epigenetic modifications due to the structure of their promoters. UR - https://ijbms.mums.ac.ir/article_1116.html L1 - https://ijbms.mums.ac.ir/article_1116_fb39c7733ab44ca4db3045286816801d.pdf ER -