TY - JOUR ID - 11203 TI - Mangosteen peel extract (Garcinia mangostana L.) as protective agent in glucose-induced mesangial cell as in vitro model of diabetic glomerulosclerosis JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Widowati, Wahyu AU - Laksmitawati, Dian AU - Wargasetia, Teresa AU - Afifah, Ervi AU - Amalia, Annisa AU - Arinta, Yukko AU - Rizal, Rizal AU - Suciati, Tri AD - Faculty of Medicine, Maranatha Christian University, Bandung 40164, West Java, Indonesia AD - Faculty of Pharmacy, Pancasila University, Jakarta Selatan 12630, DKI Jakarta, Indonesia AD - Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung 40163, West Java, Indonesia AD - School of Pharmacy Bandung Insitute of Technology, Bandung 40132, West Java, Indonesia Y1 - 2018 PY - 2018 VL - 21 IS - 9 SP - 972 EP - 977 KW - Fibronectin KW - Glomerulosclerosis KW - Garcinia mangostana KW - Mesangial cell KW - Transforming growth factor-β1 DO - 10.22038/ijbms.2018.29349.7094 N2 - Objective(s): This study aims to evaluate the activity of mangosteen peels extract (MPE) as protection agent on induced-glucose mesangial cells (SV40 MES 13 cell line (Glomerular Mesangial Kidney, Mus Musculus)).  Materials and Methods: MPE was performed based on maceration method. Cytotoxic assay was performed based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) method, while the level of TGF-β1 (Transforming growth factor-β1) and fibronectin in glucose-induced mesangial cells were assayed and determined using ELISA KIT. Results: In viability assay, MPE 5 and 20 µg/ml has the highest activity to increase cells proliferation in glucose-induced mesangial cells at 5, 10, and 15 days of incubation in glucose concentration (5 and 25 mM) (P<0.05). In inhibitory activity of TGF-β1 and fibronectin level, MPE 5 µg/ml (glucose-induced 5 mM) show the lowest level compared to positive control and other treatments (P<0.05).Conclusion: MPE can increase cell proliferation in glucose-induced mesangial cells and significantly reduce the level of TGF-β1 and fibronectin. MPE activity has correlates to inhibit the diabetic glomerulosclerosis condition and may increase mesangial cell proliferation. UR - https://ijbms.mums.ac.ir/article_11203.html L1 - https://ijbms.mums.ac.ir/article_11203_14e704ac2a5f60db9c0879be5810f630.pdf ER -