TY - JOUR ID - 11914 TI - Oral administration of Ginkgolide B alleviates hypoxia-induced neuronal damage in rat hippocampus by inhibiting oxidative stress and apoptosis JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Li, Wang AU - Qinghai, Shi AU - Kai, Li AU - Xue, Ma AU - Lili, Niu AU - Jihua, Ran AU - Zhengxiang, Liu AU - Xiaoling, Li AU - Di, Ge AU - Qi, Yang AU - Mengyun, Deng AU - Jianfeng, Fu AD - Clinical Laboratory Diagnostic Center, General Hospital of Xinjiang Military Region, Urumqi 830000, Xinjiang, China AD - Clinical Laboratory Diagnostic Center, Changji State People’s Hospital, Changji, Xinjiang, China AD - Clinical Laboratory Diagnostic Center, Sichuan Provincial People’s Hospital , Chengdu, Sichuan, China Y1 - 2019 PY - 2019 VL - 22 IS - 2 SP - 140 EP - 145 KW - Apoptosis KW - Ginkgolide B KW - Hippocampus KW - Hypoxia KW - Oxidative stress injury DO - 10.22038/ijbms.2018.26228.6569 N2 - Objective(s): The aim of this study is to explore the potential neuroprotective effects of Ginkgolide B (GB), a main terpene lactone and active component in Ginkgo biloba, in hypoxia-induced neuronal damage, and to further investigate its possible mechanisms.Materials and Methods: 54 Sprague-Dawley rats were randomly divided into three groups: the untreated control group (n=18); the hypoxia group (n=18; exposed to 6000 m simulated plateau altitude for six days); and the GB group (n=18; intragastric administration of 12 mg/kg GB three days prior to rapid adaption to 6000 m and on the first two days of hypoxia). After hypoxia exposure for six days, we dissected out the brain hippocampi and performed hematoxylin and eosin staining, Nissl staining, and TUNEL staining. Homogenates of the hippocampi were used to test the oxidative stress indices including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), and catalase. Bax and caspase-3 expression in the hippocampal tissue was measured using qRT-PCR.Results: Treatment with GB before exposure to hypoxia could protect neural cells and increase the number of Nissl bodies. TUNEL and qRT-PCR results demonstrated that GB treatment could decrease apoptotic cells in different areas of the hippocampus. Antioxidant defense systems such as SOD, GSH, and catalase were decreased (P<0.05), and the concentration of MDA was reduced significantly in the hippocampi of rats of the GB group (P<0.05).Conclusion: GB could alleviate hypoxia-induced neuronal damage in rat hippocampus by inhibiting oxidative stress and apoptosis. UR - https://ijbms.mums.ac.ir/article_11914.html L1 - https://ijbms.mums.ac.ir/article_11914_4110e4508b267cd8003c0310d534a1cb.pdf ER -