TY - JOUR ID - 12011 TI - Extremely low frequency-pulsed electromagnetic fields affect proangiogenic-related gene expression in retinal pigment epithelial cells JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Oladnabi, Morteza AU - Bagheri, Abouzar AU - Rezaei Kanavi, Mozhgan AU - Azadmehr, Abbas AU - Kianmehr, Anvarsadat AD - Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran AD - Department of Clinical Biochemistry and Genetics, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran AD - Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AD - Immunology Department, Babol University of Medical Sciences, Babol, Iran AD - Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran Y1 - 2019 PY - 2019 VL - 22 IS - 2 SP - 128 EP - 133 KW - ELF-PEMF KW - Gene expression KW - Proangiogenic factors KW - Quantitative real-time PCR KW - RPE cells DO - 10.22038/ijbms.2018.25023.6214 N2 - Objective(s): It is known that extremely low frequency-pulsed electromagnetic fields (ELF-PEMF) influence multiple cellular and molecular processes. Retinal pigment epithelial (RPE) cells have a significant part in the emergence and pathophysiology of several ocular disorders, such as neovascularization. This study assessed the impact of ELF-PEMF on the proangiogenic features of RPE cells. Materials and Methods: Primary cultured RPE cells were treated with ELF-PEMF (50 Hz) for three days. Using ELISA assay, we evaluated the effects of treatment on RPE cell proliferation and apoptosis. Also, RT-PCR was used to determine the gene expression of proangiogenic factors, such as matrix metalloproteinase-2 (MMP-2), MMP-9, vascular endothelial growth factors receptor 2 (VEGFR-2), hypoxia-inducible factor 1 (HIF-1α), VEGFA, cathepsin D, connective tissue growth factor (CTGF), E2F3, tissue inhibitors of metalloproteinases 1 (TIMP-1), and TIMP-2.Results: No noticeable changes were observed in cell proliferation and cell death of ELF-PEMF-exposed RPE cells, while transcript levels of proangiogenic genes (HIF-1α, VEGFA, VEGFR-2, CTGF, cathepsin D, TIMP-1, E2F3, MMP-2, and MMP-9) increased significantly.Conclusion: RPE cells are important for homeostasis of the retina. ELF-PEMF increased the gene expression of proangiogenic factors in RPE cells, which highlights concerns about the impact of this treatment on human health. UR - https://ijbms.mums.ac.ir/article_12011.html L1 - https://ijbms.mums.ac.ir/article_12011_22332795f2464a3564238d312162a47c.pdf ER -