TY - JOUR ID - 12770 TI - Iron chelation effect of curcumin and baicalein on aplastic anemia mouse model with iron overload JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Wu, Dijiong AU - Wen, Xiaowen AU - Xu, Linglong AU - Liu, Wenbin AU - Hu, Huijin AU - Ye, Baodong AU - Zhou, Yuhong AD - Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China AD - Department of Internal Medicine, Central Hospital of Jinhua Affiliated to Zhejiang University, Jinhua, Zhejiang, China AD - Department of Hematology, Taizhou Central Hospital, Taizhou, Zhejiang, China Y1 - 2019 PY - 2019 VL - 22 IS - 6 SP - 660 EP - 668 KW - Anemia KW - Animal KW - Aplastic KW - Baicalein KW - Curcumin KW - Deferoxamine KW - Iron overload KW - Mice KW - Models DO - 10.22038/ijbms.2019.30840.7440 N2 - Objective(s): The current work aimed to assess whether curcumin and baicalein can chelate iron in aplastic anemia (AA) complicated with iron overload, exploring the potential mechanisms.Materials and Methods: A mouse model of AA with iron overload complication was firstly established. Low and high-dose curcumin or baicalein treatment groups were set up, as well as the deferoxamine positive control, normal and model groups (n=8). Hemogram and bone marrow mononuclear cell detection were performed, and TUNEL and immunohistochemical staining were used to evaluate hematopoiesis and apoptosis in the marrow. ELISA, Western blot, and qRT-PCR were employed to assess serum iron (SI), serum ferritin (SF), bone morphogenetic protein 6 (BMP-6), SMAD family member4 (SMAD4) and transferrin receptor 2 (TfR2) amounts. Results: Both curcumin and baicalein improved white blood cell (increase of 0.28-0.64×109/l in high-dose groups) and hemoglobin (increase of around 10 g/l) amounts significantly, which may related to decreased apoptosis (nearly 30%-50% of that in the model group) in the bone marrow, while their effects on platelet recovery were limited and inferior to that of deferoxamine (DFO). Both test compounds up-regulated hepcidin and its regulators (BMP-6, SMAD, and TfR2) at the protein and mRNA levels; high dosage treatment may be beneficial, being better than DFO administration in lessening iron deposition in the bone marrow.Conclusion: Curcumin and baicalein protect hematopoiesis from immune and iron overload-induced apoptosis, exerting iron chelation effects in vivo. UR - https://ijbms.mums.ac.ir/article_12770.html L1 - https://ijbms.mums.ac.ir/article_12770_1e0d52f05545e5352aca6eac7872d4e9.pdf ER -