TY - JOUR ID - 13346 TI - The impact of Lactobacillus acidophilus on hepatic and colonic fibrosis induced by ethephon in a rat model JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Bahr, Hoda I AU - Hamad, Rania AU - Ismail, Shimaa A A AD - Biochemistry Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt AD - Pathology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt AD - Clinical Pathology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt Y1 - 2019 PY - 2019 VL - 22 IS - 8 SP - 956 EP - 962 KW - Ethephon KW - Immunity KW - L. acidophilus KW - NF-κB KW - α-SMA DO - 10.22038/ijbms.2019.32936.7866 N2 - Objective(s): The study is aimed to elucidate the impact of antioxidant, anti-inflammatory and antifibrosis properties of Lactobacillus acidophilus (L. acidophilus) on liver and colon in ethephon treated rats through measuring Pro- inflammatory cytokines, oxidative stress index, lysosomal cathepsin-D enzyme activity and fibrosis markers.Materials and Methods: Rats divided into three groups; Group 1: distilled water control, Group 2: rats at day 16 from experiment beginning were orally received ethephon 50 mg/ kg BW in distilled water once daily for 60 days.  Group 3: rats were orally received L. acidophilus enriched diet 1% (w/w) for 15 days as prophylactic, then received both L. acidophilus enriched diet 1% (w/w) and ethephon 50 mg/kg BW for 60 days. Results: Ethephon exerts hepatic and colonic oxidative stress, inflammatory response and fibrosis through NF-κB activation. On contrary, L. acidophilus supplementation evokes hepatoprotective properties as revealed by decreased serum AST, ALT, γ -GT and increased IGF-1. L. acidophilus exerts antioxidant and anti-inflammatory properties as indicated by decreased TOS, OSI, TNF-α, IL-1β, cathepsins D activity, NF-κB expression and increased TAC, lysosomal membrane stability. L. acidophilus shows antifibrotic activity as demonstrated by down-regulation of TGF-β1, α-SMA, collagen expression. Conclusion: L. acidophilus possess antioxidant, anti -inflammatory and antifibrotic activity through inhibition of NF-kB. UR - https://ijbms.mums.ac.ir/article_13346.html L1 - https://ijbms.mums.ac.ir/article_13346_d9010a666af56e53f64b6e2d952fb6a8.pdf ER -