TY - JOUR ID - 1348 TI - Effect of Melatonin on the Expression of Apoptotic Genes in Vitrified-thawed Spermatogonia Stem Cells Type A of 6-Day-Old Mice JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Gholami, Mohammadreza AU - Saki, Ghasem AU - Hemadi, Masoud AU - Khodadadi, Ali AU - di-asl, Javad Mohamma AD - Department of Anatomy, Lorestan University of Medical Sciences, Khoramabad, Iran AD - Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran AD - Fertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran AD - Department of Immunology, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran AD - Departmentof Medical Genetics, Ahvaz Jundishapur University of Medical Sciences(AJUMS), Ahvaz, Iran Y1 - 2013 PY - 2013 VL - 16 IS - 8 SP - 906 EP - 909 KW - Apoptosis Melatonin Testes Vitrification DO - 10.22038/ijbms.2013.1348 N2 -  Objective(s):  Being secreted by the pineal gland, melatonin induces cell proliferation in normal cells and induced apoptosis in cancer cells. The purpose of this study was to investigate effects of melatonin on main components and the expression of apoptotic genes in vitrified-thawed testicular germ cells of 6- day–old mice.  Materials and Methods: Testes of neonate Balb/c mice were vitrified- thawed under standard condition with or without the addition of 100 μM melatonin to both vitrification and thawing solutions. Subsequently, Vitrified-thawed wholetestes were digested under standard condition and spermatogonial stem cells type A were separate in the suspension with CD90.1 (Thy1.1+) micro beads. Extraction of RNA and synthesis of cDNA was performed. Expression levels of apoptotic genes (Fas, P53, BCL-2 and BAX) were determined using Real-time PCR. Results: With all genes being expressed, level of expression for Fas was higher and for that of P-53 was lower than the remaining genes. Conclusion: Melatonin may cause apoptosis in cells being damaged under the influence of freezing thawing process. In order to examine the exact effects of melatonin on spermatogonia stem cells apoptosis, additional studies are required. UR - https://ijbms.mums.ac.ir/article_1348.html L1 - https://ijbms.mums.ac.ir/article_1348_9706f3a9e4a246a1a85331048a1642f5.pdf ER -