TY - JOUR ID - 14823 TI - Prevalence of plasmid-encoded carbapenemases in multi-drug resistant Escherichia coli from patients with urinary tract infection in northern Iran JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Deldar Abad Paskeh, Mahshid AU - Mehdipour Moghaddam, Mohammad Javad AU - Salehi, Zivar AD - University of Guilan, University Campus 2, Rasht, Iran AD - Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran Y1 - 2020 PY - 2020 VL - 23 IS - 5 SP - 586 EP - 593 KW - Carbapenemase KW - Cephalosporines KW - Multiplex PCR KW - Plasmid KW - Resistance DO - 10.22038/ijbms.2020.34563.8199 N2 - Objective(s): Resistance to carbapenems as the last line for controlling resistant bacteria is increasing due to production of carbapenemase. The aim of this study was to detect the plasmid-encoded carbapenemases using phenotypic methods and multiplex PCR among the multi-drug resistant (MDR) isolates from patients with urinary tract infection (UTI) in northern Iran.Materials and Methods: Antimicrobial susceptibility testing and extended spectrum β-lactamase (ESBL) production test were performed for 91 MDR Escherichia coli strains by disc diffusion and double disk synergy tests (DDST), respectively. Carbapenemases production was confirmed using Hodge test, EDTA double disk synergy test (EDST) and combined disk test (CDT). The isolates were subjected to PCR targeting blaIMP, blaVIM, blaKPC and blaOXA-48 β-Lactamase genes. Results: Resistance of isolates to 1st, 2nd, 3rd, and 4th generations of cephalosporins, carbapenems, and penicillins were 73%, 84.5%, 62%, 37.5%, 17.5%, and 76%, respectively. Based on CDT and Hodge test, 1 (3%) and based on EDST, 2 (6%) of 33 ESBL producers synthesize a type of carbapenemase. The frequency of blaIMP, blaVIM, blaKPC, and blaOXA-48 genes was 8.7%, 9.8%, 2.1%, and 15.3%, respectively. Existence of blaIMP conferred more resistance to cephalotin, fosfomycin, and piperacillin (P≤0.01) and carrying blaVIM caused more resistance to cephalotin, cefepime, and ceftazidime (P≤0.01). The presence of blaKPC conferred more resistance to cephalotin and presence of blaOXA-48 caused more resistance to chloramphenicol and piperacillin (P≤0.05).Conclusion: Identification and controlling of this nearly low frequent ESBL and carbapenemase producing strains are important due to the presence of plasmid genes encoding carbapenemase. UR - https://ijbms.mums.ac.ir/article_14823.html L1 - https://ijbms.mums.ac.ir/article_14823_aa62e003e5035647aa1391317d12c769.pdf ER -