TY - JOUR ID - 15834 TI - Bioinformatics prediction and experimental validation of VH antibody fragment interacting with Neisseria meningitidis factor H binding protein JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Rafighdoust, Hediyeh AU - Ahangarzadeh, Shahrzad AU - Yarian, Fatemeh AU - Taheri, Ramezan Ali AU - Lari, Arezou AU - Bandehpour, Mojgan AU - Salahshoor Dahr, Mona AD - Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran AD - Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran AD - Cellular & Molecular Biology Research Center Shahid Beheshti University of Medical Sciences, Tehran, Iran AD - Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences,Tehran, Iran AD - Systems Biomedicine, Pasteur Institute of Iran, Tehran, Iran AD - Department of Biology, Faclty of Basic Science, Islamic Azad University Islamshahr Branch, Islamshahr, Iran Y1 - 2020 PY - 2020 VL - 23 IS - 8 SP - 1053 EP - 1058 KW - Cloning KW - Heavy chain variable KW - Modeling KW - Molecular docking KW - Surface Plasmon Resonance DO - 10.22038/ijbms.2020.44007.10318 N2 - Objective(s): We previously conducted an in silico research on the interactions between the ribosome display-selected single chain variable fragment (scFv) and factor H binding protein (fHbp) of Neisseria meningitidis. We found that heavy chain variable (VH) fragment of this scFv had considerable affinity to fHbp. These results led us to evaluate the ability of this small antibody fragment in binding and detection of fHbp antigen.Materials and Methods: In this study, at first, the three-dimensional structure of VH fragment was simulated by Kotai Antibody Builder web server. By using ClusPro 2.0 web server, the 3D structure of the soluble form of fHbp (PDB: 2KC0) was docked to the modeled VH fragment to extract the structure of the complex’s binding. Molecular dynamics (MD) simulation was carried out using GROMACS 4.5.3 package for 65 ns. Secondly, coding sequence of VH fragment was cloned separately and expressed in Escherichia coli. After purification of the VH fragment, its binding activity to fHbp protein was analyzed by enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) method.Results: Important amino acids involved in antigen- antibody interaction were identified by analyzing the fHbp-VH complex. The ability of the VH antibody fragment to bind and detect fHbp antigen has been confirmed by the results of in silico analysis, ELISA and SPR methods.Conclusion: These results showed that this small fragment of antibody could be used for designing diagnostic kits. UR - https://ijbms.mums.ac.ir/article_15834.html L1 - https://ijbms.mums.ac.ir/article_15834_fb1a1b340c2fd8fefbadbda848d08ffd.pdf ER -