TY - JOUR ID - 16139 TI - Naphthoquinones from Handroanthus impetiginosus promote skin wound healing through Sirt3 regulation JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Ahmad, Fayyaz AU - Bibi, Shaheen AU - Kang, Mincheol AU - Anees, Mariam AU - Ansar, Muhammad AU - Alam, Muhammad Rizwan AU - Kim, Sun AU - Wahedi, Hussain Mustatab AD - Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, 45320 Islamabad, Pakistan AD - College of Pharmacy, Gachon University, 191 Hambakmaero, Incheon, South Korea AD - Department of Biological Sciences, National University of Medical Sciences, C/O Military Hospital, Mall Road Rawalpindi, Pakistan Y1 - 2020 PY - 2020 VL - 23 IS - 9 SP - 1139 EP - 1145 KW - Beta KW - Lapachone Dermatology Inflammation Regeneration Tabebuia DO - 10.22038/ijbms.2020.43706.10275 N2 - Objective(s): Lapachone is a natural naphthoquinone-derived compound found in Tabebuia avellanedae. It is well-known for its analgesic, anti-inflammatory, anti-microbial, diuretic, and anti-cancerous effects. However, the wound-healing effects of this compound are not known yet. The aim of this study was to investigate the wound healing activity of naphthoquinones (α-lapachone and β-lapachone) from Handroanthus impetiginosus. Materials and Methods: Expression of Sirt3, migration-related proteins (Rac1, Cdc42, α-Pak) and angiogenesis-related protein of vascular endothelial growth factor (VEGF) was monitored using western blot analysis. Blood vessel formation and tissue development were monitored by angiogenesis assay and hematoxylin & eosin (H & E) staining, respectively on mouse skin tissue samples. Both α-lapachone and β-lapachone increased Sirt3 expression in vivo, but only β-lapachone increased Sirt3 expression in vitro. Results: Both the compounds accelerated wound healing in cultured skin cells as well as mouse skin; however, β-lapachone was more effective at lower concentrations. Both of the compounds increased the expression of migration-related proteins both in vitro and in vivo. Similarly, α-lapachone and β-lapachone increased VEGF expression, tissue development and blood vessel formation in mouse skin.Conclusion: These findings indicated that α-lapachone and β-lapachone are novel Sirt3 activators, and Sirt3 has a role in wound healing. Thus, Sirt3 and its regulators come out as a novel target and potential drug candidates, respectively in the important field of cutaneous wound healing. UR - https://ijbms.mums.ac.ir/article_16139.html L1 - https://ijbms.mums.ac.ir/article_16139_c2c8bb6f3e32bc19e9c5dd1f36db3e9f.pdf ER -