TY - JOUR ID - 1985 TI - An Efficient Covalent Coating on Glass Slides for Preparation of Optical Oligonucleotide Microarrays JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Pourjahed, Atefeh AU - Rabiee, Mohammad AU - Tahriri, Mohammadreza AD - Biomaterials Group (Center of Excellence in Biomaterials), Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran 2 Nanobiotechnology center, Department of Chemical Engineering, Jondishapour University of Technology, Dezful, Iran AD - Biomaterials Group (Center of Excellence in Biomaterials), Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran Y1 - 2013 PY - 2013 VL - 16 IS - 12 SP - 1259 EP - 1265 KW - Agarose KW - Coating KW - Microarray KW - PLL KW - Signal-to-noise DO - 10.22038/ijbms.2013.1985 N2 - Objective(s): Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.   Materials and Methods: In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated agarose linked covalently to PLL amine groups; also bound to the amino groups of biomolecules. These linkages were fixed by UV irradiation. The prepared substrates were compared to only agarose-coated and PLL-coated slides.   Results: Results on atomic force microscope (AFM) demonstrated that agarose provided three-dimensional surface which had higher loading and bindig capacity for biomolecules than PLL-coated surface which had two-dimensional surface. In addition, the signal-to-noise ratio in hybridization reactions performed on the agarose-PLL coated substrates increased two fold and four fold compared to agarose and PLL coated substrates, respectively.   Conclusion: The agarose-PLL microarrays had the highest signal (2546) and lowest background signal (205) in hybridization, suggesting that the prepared slides are suitable in analyzing wide concentration range of analytes.   UR - https://ijbms.mums.ac.ir/article_1985.html L1 - https://ijbms.mums.ac.ir/article_1985_f51c89b165ade809284acffab67d069e.pdf ER -