TY - JOUR ID - 2583 TI - Spectrofluorimetric determination of cefixime using terbium-danofloxacin probe JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Manzoori, Jamshid L AU - Amjadi, Mohammad AU - Soltani, Naser AU - Jouyban, Abolghasem AD - Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran AD - Tuberculosis and Lung Disease Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran AD - Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran Y1 - 2014 PY - 2014 VL - 17 IS - 4 SP - 256 EP - 262 KW - Cefixime KW - Danofloxacin KW - Quenching KW - Spectrofluorimetry KW - Terbium-sensitized DO - 10.22038/ijbms.2014.2583 N2 - Objective(s):Cefixime (Cfx), is a semi-synthetic third-generation oral cephalosporin antibiotic that is prescribed for the treatment of susceptible infections. There are some procedures for the determination of Cfx in pharmaceutical formulations and biological samples. Herein a spectrofluorimetric method was proposed for Cfx determination based on the fluorescence quenching of terbium-danofloxacin (Tb3+-Dano) in the presence of Cfx. Materials and Methods: Cfx was detected based on fluorescence quenching of terbium-danofloxacin (Tb3+-Dano) in the presence of Cfx with maximum excitation and emission wavelengths at 347 nm and 545 nm, respectively. The quenched fluorescence intensity of Tb3+- Dano system is proportional to the concentration of Cfx. The optimum conditions for the determination of Cfx were studied. Results: The maximum response was achieved under optimum conditions of [Tris buffer]= 0.008 mol/l (pH 6.5), [Tb3+]=1×10-4 mol/l  and [Dano]=1×10-4 mol/l. The developed method was evaluated in terms of accuracy, precision and limit of detection. The linear concentration ranges for quantification of Cfx were 8.8×10-8-8.8×10-7 mol/l and 1.1×10-7-8.8×10-7 mol/l in standard and human serum samples with the detection limits (S/N=3) of 2.8×10-8 mol/l and 3.9×10-8 mol/l, respectively. The Cfx was determined in pharmaceutical tablets and spiked serum samples and the results were satisfactory.   Conclusion: This method is simple, practical and relatively interference-free for determination of Cfx in pharmaceutical tablets and serum samples. UR - https://ijbms.mums.ac.ir/article_2583.html L1 - https://ijbms.mums.ac.ir/article_2583_c50ef63c69c45aa81edbb16f28085460.pdf ER -