TY - JOUR ID - 4283 TI - Immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from Brucella melitensis JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Amani, Jafar AU - Ghasemi, Amir AU - Ranjbar, Reza AU - Shabani, Mahdi AU - Zandemami, Mahdi AU - Golmohammadi, Reza AD - Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran AD - Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran AD - Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran Y1 - 2015 PY - 2015 VL - 18 IS - 4 SP - 350 EP - 355 KW - Brucella melitensis KW - ELISA KW - Fusion protein KW - Immune reactivity KW - Vaccine DO - 10.22038/ijbms.2015.4283 N2 - Objective(s): Brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. Immunity against Brucella melitensis involves antigen-specific CD4+ and CD8+ T-cells activation and humoral immune responses. Due to negative aspects of live attenuated vaccines, much attention has been focused on finding Brucella-protective antigens to introduce them as potential subunit vaccine candidates. Materials and Methods: A chimeric gene encoding trigger factor (TF), Omp3148-74 and BP2687-111 fragments (TOB) from B. melitensis was successfully cloned, expressed in Escherichia coliBL21-DE3 and purified by Ni-NTA agarose column. Antibodies to recombinant TOB (rTOB) have been investigated in Brucella-infected human sera and a pool serum prepared from B. melitensis-vaccinated rabbits. Results: Our results showed that the immunized rabbit pool serum strongly reacted with rTOB. In addition, antibodies against rTOB were detectable in 76.5% of sera obtained from infected patients. Conclusion: These findings suggest that rTOB may provide a potential immunogenic candidate which could be considered in future vaccine studies. UR - https://ijbms.mums.ac.ir/article_4283.html L1 - https://ijbms.mums.ac.ir/article_4283_18470303f57a9677ceb20cd27c7e6083.pdf ER -