TY - JOUR ID - 4539 TI - The preventive effects of dexmedetomidine against intestinal ischemia-reperfusion injury in Wistar rats JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Zhang, Xue-kang AU - Zhou, Xiao-ping AU - Zhang, Qin AU - Zhu, Feng AD - Department of Anesthesiology, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China AD - Grade 2012 of Medical Department of Graduate School, Nanchang University, Nanchang, Jiangxi 330006, China AD - Intensive Care Unit (ICU), First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China Y1 - 2015 PY - 2015 VL - 18 IS - 6 SP - 604 EP - 609 KW - Dexmedetomidine Inflammatory–response KW - Intestinal injury KW - Ischemia-reperfusion DO - 10.22038/ijbms.2015.4539 N2 - Objective(s): Intestinal ischemia-reperfusion is a major problem, which may lead to multiorgan failure and death. The aim of this study was to evaluate the protective effects of dexmedetomidine on cell proliferation, antioxidant system, cell death, and structural integrity in intestinal injury induced by ischemia-reperfusion in rats. Materials and Methods: Animals were randomized into three groups: group A, sham-operated or control; group B, intestinal ischemia/reperfusion (IR); and group C, intestinal IR pretreated with 50 μg of dexmedetomidine. Intestine tissue was collected from all rats 30 min after desufflation, and fresh frozen for histological and biochemical evaluation. Results: The intestinal tissue of group B rats showed a significant decrease in the antioxidant             enzyme activities. However, these enzyme activities were improved by the administration of dexmedetomidine. Inhibiting the protein expression of MCP7, PAR2, P-JAK, P-STAT1, and P-STAT3 proved the protective effect of dexmedetomidine. The immunohistochemical staining revealed its protective effect by maintaining the normal structural integrity, less caspase-3 immuno reactivity, and increased cell proliferation count in the intestinal tissues. Conclusions: Intraperitoneal injection of dexmedetomidine significantly protected intestine IR injury in rats by inhibiting the inflammatory response, intestinal epithelial apoptosis, and maintaining structural integrity of intestinal cells. UR - https://ijbms.mums.ac.ir/article_4539.html L1 - https://ijbms.mums.ac.ir/article_4539_a420273f729430dba3cfa80aee4a05c0.pdf ER -