TY - JOUR ID - 4833 TI - Safety Assessment of Ocimum Basilicum Hydroalcoholic Extract in Wistar Rats: Acute and Subchronic Toxicity Studies JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Rasekh, Hamid Reza AU - Hosseinzadeh, Leila AU - Mehri, Soghra AU - Kamli-Nejad, Mohammad AU - Aslani, Majid AU - Tanbakoosazan, Farahnaz AD - School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran AD - School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran AD - School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran Y1 - 2012 PY - 2012 VL - 15 IS - 1 SP - 645 EP - 653 KW - acute toxicity KW - Ocimum basilicum KW - Rats KW - Subchronic toxicity DO - 10.22038/ijbms.2012.4833 N2 - Objective(s) Ocimum basilicum L. is widely used in folk medicine of many countries including Iran. Both O. basilicum and its oil extract have received considerable attention for their potential medicinal properties, but there are a few reports about possible toxicity of this plant. Therefore, in the present study, acute and subchronic toxicity of O. basilicum hydroalcohlic extract have been evaluated in Wistar rats. Materials and Methods For the acute toxicity assessment, five groups of 10 animals (5 male, 5 female) received four different single dose of extract orally, the animals were, then, kept under observation for 14 days. For subchronic toxicity, the animals were divided into four groups (5 male, 5 female) and were gavaged daily by 50, 200 and 500 mg/kg of extract.  Mortality, clinical signs, body weight changes, food and water consumption, and hematological and biochemical parameters were monitored during the study period. On the 45th day, animals were sacrificed and gross findings, weight of liver and left kidney and liver histological markers were assessed. Results The results of acute study indicated that LD50 of O. basilicum is higher than 5 mg/kg. In subchronic study, no adverse effects were observed on serum parameters in male and female rats. The hematological results showed a reduction in the hematocrit, platelets and RBC in both sexes. No abnormalities were observed in other parameters. Conclusion Based on the results of this study, present data suggest that hematologic system could serve as a target organ in oral toxicity of this plant. UR - https://ijbms.mums.ac.ir/article_4833.html L1 - https://ijbms.mums.ac.ir/article_4833_9f71d6cb80db080ef5e6005ecc49391d.pdf ER -