TY - JOUR ID - 5082 TI - A Functional Polymorphism of the Granulocyte Macrophage Colony Stimulating Factor is not Associated with the Outcome of HTLV-I Infection JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Shirdel, Abbas AU - Rafatpanah, Houshang AU - Rahimi, Hassan AU - Rezaee, Abdol Rahim AU - Azarpajooh, Mahmoud Reza AU - Beyk yazdi, Akram AU - V Hutchinson, Ian AD - Internal Medicine Department, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran AD - Immunology Research Centre, BuAli Reserch Institute, Mashhad University of Medical Sciences, Mashhad, Iran AD - Neurology Department, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran AD - Immunology Research Group, Faculty of Life Sciences, the University of Manchester, UK Y1 - 2010 PY - 2010 VL - 13 IS - 2 SP - 40 EP - 47 KW - Electrophoretic Mobility Shift Assay KW - Gene Polymorphisms KW - Granulocyte-Macrophage Colony-Stimulating Factor KW - Human T-lymphotropic virus 1 DO - 10.22038/ijbms.2010.5082 N2 - Introduction Genetic background has known to be associated with the outcome of human T cell lymphotropic virus (HTLV) type I infection. In The present study we investigate the association between GM-CSF gene polymorphisms with the outcome of HTLV-I infection. Materials and Methods We analyzed 3 single-nucleotide polymorphisms in the promter region of granulocyte macrophage colony stimulating factor (GM-CSF) at positions -677*A/C, -1440*A/G and -1916*T/C in 68 patients with HTLV- I-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and 77 HTLV-I-seropositive asymptomatic carriers and 175 healthy controls from an area in Iran, Mashhad, where HTLV-I is endemic. Results No significant differences were observed in the distribution of GM-CSF polymorphisms between HAM/TSP patients, HTLV-I carriers and healthy controls (P> 0.05). The -677*A/C polymorphism fall within the transcriptional enhancer factor-2 (TEF-2) binding site, so an electrophoretic mobility shift assay (EMSA) was performed to determine the effects of polymorphisms on protein binding to the GM-CSF promoter. The result showed a significantly higher binding efficiency of nuclear protein to the A allele compared with the C allele. Conclusion Our study suggests that polymorphisms in the GM-CSF promoter is not associated with the outcome of HTLV-I infection, however, GM-CSF polymorphism at position -677 could indeed influence gene expression. UR - https://ijbms.mums.ac.ir/article_5082.html L1 - https://ijbms.mums.ac.ir/article_5082_5884db05a8983be86eefdb04a4048723.pdf ER -