TY - JOUR ID - 5180 TI - Effect of Shark Liver Oil on Peritoneal Murine Macrophages in Responses to Killed-Candida albicans JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Hajimoradi, Monire AU - Daneshmandi, Saeed AU - Hassan, Zuhair Mohammad AU - Roudbary, Maryam AD - Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran AD - Department of Mycology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran Y1 - 2009 PY - 2009 VL - 12 IS - 3 SP - 179 EP - 183 KW - Candida albicans KW - Macrophage KW - Shark KW - Liver Oil DO - 10.22038/ijbms.2009.5180 N2 - Objective(s) Shark Liver Oil (SLO) is an immunomodulator. Macrophages play a key role in host defense against pathogens like fungi. Candida albicans have mechanisms to escape immune system. We determined the effect of killed-Candida on the in vitro viability of macrophages and the effect of SLO on augmentation of this potency. Materials and Methods Peritoneal macrophages were separated and cultured (3*105/well). At first, the effect of killed-Candida (200 cells/well) on macrophage viability was evaluated, using MTT test. Then, MTT was performed on macrophages stimulated with killed-Candida in the presence of SLO. Results Killed-Candida suppressed the ability of MTT reduction and hence macrophages viability (P=0.026), but addition of SLO (100 mg/ml) significantly enhanced cell viability (P=0.00). So, SLO could neutralize the inhibitory effect of Candida. Conclusion Simultaneous with cytotoxic effect of killed-Candida cells on macrophages viability, SLO augment macrophages viability. So, it can be applied in candidiasis as a complement. UR - https://ijbms.mums.ac.ir/article_5180.html L1 - https://ijbms.mums.ac.ir/article_5180_3998da248577fec648a1209edfc7d29b.pdf ER -