TY - JOUR ID - 5280 TI - Quantification Analysis of Dot Blot Assays for Human Immunodeficiency Virus Type 1 and 2 Antibodies JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Ravanshad, M. AU - Sabahi, F. AU - Mahboudi, F. AD - Department of Virology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran AD - Biotechnology Research Center, Pasture Institute of Iran, Tehran, Iran Y1 - 2007 PY - 2007 VL - 10 IS - 2 SP - 132 EP - 138 KW - Densitometric Analysis KW - Dot Blot assay KW - Human immunodeficiency virus DO - 10.22038/ijbms.2007.5280 N2 - Objective Dot Blot (DB) assay provides highly specific results, but usually not reliable for quantification of antibody production. The need for a more objective DB assay to provide a better definition of the immune status, against HIV antigens, promoted this study to be done to develop a quantitative DB assay. Material and Methods Dot blot (DB) strips for antibodies directed to human immunodeficiency virus (HIV) type 1 and 2 were analyzed by a video densitometer. This method was used to quantify the antibody response to different HIV proteins in infected patients. In order to increase reproducibility, reagents and protocols were accurately standardized and internal controls were added. In the first format, an internal control band consisting of Human IgG was added to each dot to minimize the effects of band intensity variation. In the second format, antibody concentrations were calculated from the ratio of the densities produced by test sera and by positive and negative standard sera. Results The sera under scrutiny were also examined by standard enzyme-linked immunosorbent assay (ELISA) and the obtained results were compared with those of the corresponding DB. A statistically significant positive correlation was found between the results obtained with the two methods, and this was especially evident when ELISA titers were compared to corrected DB values (p = 0.001). Conclusion Densitometric analysis of DB assays led to quantify the antibodies against HIV-1 and 2 Gag and Env proteins and might be useful to investigate possible humoral immune correlates of production in HIV vaccine studies and antibody production in the early phase of infection.    UR - https://ijbms.mums.ac.ir/article_5280.html L1 - https://ijbms.mums.ac.ir/article_5280_af22adff21f5e2f1d435941460e79f53.pdf ER -