TY - JOUR ID - 6805 TI - Tracheal overexpression of IL-1β, IRAK-1 and TRAF-6 mRNA in obese-asthmatic male Wistar rats JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Aslani, Mohammad Reza AU - Keyhanmanesh, Rana AU - Khamaneh, Amir Mehdi AU - Abbasi, Mehran Mesgari AU - Fallahi, Maryam AU - Alipour, Mohammad Reza AD - Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AD - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AD - School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran AD - Department of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran Y1 - 2016 PY - 2016 VL - 19 IS - 4 SP - 350 EP - 357 KW - Asthma KW - IL-1β KW - IRAK-1 KW - Obesity KW - Tracheal KW - TRAF-6 DO - 10.22038/ijbms.2016.6805 N2 - Objective(s): Human and animal studies have shown a close relationship between obesity and asthma severity. Here, we examined the effects of diet-induced obesity (DIO) on the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA in male Wistar rats tracheal after sensitization with ovalbumin (OVA). Materials and Methods:Twenty male Wistar rats divided to four groups, included, control group with normal diet (C+ND), OVA-sensitized group with normal diet (S+ND), control group with high-fat diet (C+HFD), and OVA-sensitized group with high-fat diet (S+HFD). All animals fed for 8 weeks with standard pelts or high-fat diet, and then were sensitized and challenged with OVA or saline for another 4 weeks with designed regimens. At the end of study, trachea isolated and examined for expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA with RT-PCR method. Results:Diet-induced obesity groups developed increased weight, obesity indexes and lipid profiles (P<0.05 to P<0.001). The expression levels of IL-1β mRNA in OVA-sensitization groups (S+ND and S+HFD) showed a significantly increased when compared with control group. Also in S+HFD group, expression level of TRAF-6 mRNA was higher than other groups (P<0.001). IRAK-1 expression level was high in S+HFD compared with control group.IL-1β and TRAF-6 mRNA correlated positively with obesity indexes. Conclusion:The results showed that DIO causes overexpression of IL-1β, IRAK-1 and TRAF-6 mRNA in an experimental model of asthma. Our results suggested that in obese-asthmatic conditions locally production and activation of pro-inflammatory agents can be increased. These findings showed that possible mechanism for obesity-asthma relationships. UR - https://ijbms.mums.ac.ir/article_6805.html L1 - https://ijbms.mums.ac.ir/article_6805_fce5856c822976fd81c3da30a8d33580.pdf ER -