TY - JOUR ID - 7357 TI - Repairing effects of interleukin 11 (IL-11) towards high dose methotrexate-induced rat small intestinal mucositis and its impacts on T-lymphoblastic leukemia cell line JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Han, Yueqin AU - Zhu, Yanping AU - Wang, Jinshen AU - Han, Yanqin AU - Qin, Daogang AU - Yang, Qiaozhi AU - Sun, Xiaojing AU - Chen, Lijun AD - Department of Pediatrics, Liaocheng People&#039;s Hospital, Liaocheng 252000, Shandong, China AD - Departments of Pharmacy, the Fourth People&#039;s Hospital of Liaocheng, Liaocheng 252000, Shandong, China AD - Department of Pediatrics, Shandong Province-owned Hospital, Jinan 250021, Shandong, China Y1 - 2016 PY - 2016 VL - 19 IS - 7 SP - 734 EP - 740 KW - CEM cell line KW - Interleukin 11 KW - Methotrexate KW - Mucositis DO - 10.22038/ijbms.2016.7357 N2 - Objective(s): To investigate the efficacy of interleukin 11 (IL-11) towards the high dose methotrexate (HDMTX)-concurrent rat small intestinal mucositis and its impacts on the proliferation of the human T-lymphoblastic leukemia (CEM) cell line. Materials and Methods: 95 Wistar rats were randomly divided into five groups, the normal control group (A), the methotrexate (MTX) control group (B), the IL-11-pre-treated high-dose group (C), the post-IL-11-treatment high-dose group (D) and the post-IL-11-treatment low-dose group (E). After the intraperitoneal injection of MTX in the groups B-E, the rats were sacrificed at 1, 3, 5 and 7 days. The mortality, morphological and ultrastructural changes of small intestine of each group were observed. The cells were then cultured in vitro, and the MTT method was used to investigate the effects of different concentration of IL-11 on CEM proliferation and also on HDMTX-induced mucositis. Results: IL-11 could reduce the intestinal histopathological score, increase the height of small intestinal villi, promote the proliferation of intestinal lacunar cells and reduce the mortality rate of rats. The IL-11 pre-treatment group exhibited the best efficacies, demonstrating significant difference with the control group (P<0.01). In addition, the proliferation of CEM was not promoted, indicating that IL-11 could not inhibit HDMTX. Conclusion: IL-11 could reduce the severity of HDMTX-induced intestinal mucositis, and improve the survival rate of experimental rats, and could be safely used as the adjuvant treatment of HDMTX in childhood leukemia. UR - https://ijbms.mums.ac.ir/article_7357.html L1 - https://ijbms.mums.ac.ir/article_7357_55c1e22930694a0c1351b4d53e0854a7.pdf ER -