TY - JOUR ID - 7594 TI - Recognition and characterization of Erythropoietin binding-proteins in the brain of mice JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Kowsari, Reza AU - Yazdian-Robati, Rezvan AU - Razavi, Bibi Marjan AU - Pourtaji, Atena AU - Ghorbani, Maryam AU - Moghadam-Omranipour, Hediye AU - Hosseinzadeh, Hossein AU - Lari, Parisa AU - Abnous, Khalil AD - Department of Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran AD - Targeted Drug Delivery Research Center, Department of Pharmacodinamy and Toxicology, Mashhad University of Medical Sciences, Mashhad, Iran AD - Pharmaceutical Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran AD - Department of Pharmacology and Toxicology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran AD - Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Y1 - 2016 PY - 2016 VL - 19 IS - 9 SP - 946 EP - 952 KW - Brain KW - Erythropoietin KW - Immunoprecipitation KW - Proteomic screening KW - Target deconvolution KW - Neuroprotective effect DO - 10.22038/ijbms.2016.7594 N2 - Objective(s): Erythropoietin (EPO), is a 34KDa glycoprotein hormone, which belongs to type 1 cytokine superfamily. EPO involves in erythrocyte maturation through inhibition of apoptosis in erythroid cells. Besides its main function, protective effects of EPO in heart and brain tissues have been reported. EPO has a critical role in development, growth, and homeostasis of brain. Furthermore EPO has great potential in the recovery of different brain diseases which are still under studying. In this research, EPO binding pattern to brain proteins in animal model was studied. Materials and Methods:EPO antibody was covalently crosslinked to protein A/G agarose. in order to interact between EPO  and its target in brain,  about 5µg EPO added to brain homogenates(500ul of 1 mg/ml) and incubate at 4ο C for 30 min. brain tissue lysate were added to agarose beads, After isolation of target proteins(EPO - protein) both one and two-dimensional gel electrophoresis were performed. Proteins were identified utilizing MALDI-TOF/TOF and MASCOT software. Results: This research showed that EPO could physically interact with eightproteins including  Tubulin beta, Actin cytoplasmic 2, T-complex protein 1, TPR and ankyrin repeat-containing protein 1, Centromere-associated protein E, Kinesin-like protein KIF7, Growth arrest-specific protein 2 and  Pleckstrin homology-like domain family B member 2. Conclusion: Since EPO is a promising therapeutic drug for the treatment of neurological diseases, identified proteins may help us to have a better understanding about the mechanism of protective effects of EPO in the brain. Our data needs to be validated by complementary bioassays. UR - https://ijbms.mums.ac.ir/article_7594.html L1 - https://ijbms.mums.ac.ir/article_7594_3d898942bf5768f58e185238ea7fe692.pdf ER -