TY - JOUR ID - 7737 TI - Anti-hyperglycaemic and antioxidant effects of Bidens tripartita and quantitative analysis on its active principles JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Orhan, Nilüfer AU - Gökçen İçöz, Ülkü AU - Altun, Levent AU - Aslan, Mustafa AD - Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330, Etiler, Ankara, Turkey AD - Republic of Turkey Social Security Institution, 06520, Balgat, Ankara, Turkey AD - Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06500, Tandogan, Ankara, Turkey Y1 - 2016 PY - 2016 VL - 19 IS - 10 SP - 1114 EP - 1124 KW - Antidiabetic KW - Bidens tripartite KW - Chlorogenic acid KW - Cynaroside KW - Luteolin KW - Streptozotocin DO - 10.22038/ijbms.2016.7737 N2 - Objective(s): Bidens species are used for their antidiabetic properties traditionally in many countries. Aim of this study is to evaluate hypoglycaemic and antidiabetic activity of Bidens tripartita extract and to identify its active compounds through bioactivity guided isolation technique. Materials and Methods: Hypoglycaemic effects of B. tripartita extract and its sub-extracts were investigated in normal and glucose-hyperglycaemic rats. Streptozotocin induced diabetic rats were used to examine antidiabetic activity of the extract and its sub-extracts after acute and sub-acute administration. Additionally, in vitro enzyme inhibitory and antioxidant activities were evaluated. HPLC analyses were carried out to determine the active constituents of the extract and its sub-extracts. Results: Through in vivo bioactivity-guided fractionation process, ethyl acetate and n-buthanol                  sub-extracts were found to have potent antidiabetic activity. In vitro enzyme inhibitory activities of the same sub-extracts were found to be potent. The highest total phenol, flavonoid contents and radical scavenging activity was determined in ethyl acetate sub-extract. According to LC-MS analyses, chlorogenic acid, luteolin and 7-O-glucoside of luteolin (cynaroside) were determined as the main components of the active sub-extracts. Conclusion: According to our results, B. tripartita has potent antidiabetic activity and its active constituents might be beneficial for diabetes and its complications.  UR - https://ijbms.mums.ac.ir/article_7737.html L1 - https://ijbms.mums.ac.ir/article_7737_5623f1bd805c731e8f18758d1e42316c.pdf ER -