TY - JOUR ID - 7826 TI - Ginkgetin induces apoptosis in 786-O cell line via suppression of JAK2-STAT3 pathway JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Ren, Yu AU - Huang, Shuang-shuang AU - Wang, Xue AU - Lou, Zhong-guan AU - Yao, Xu-ping AU - Weng, Guo-bin AD - Laboratory of kidney Carcinoma, Ningbo Urology and Nephrology Hospital, Urology and Nephrology Institute of Ningbo University, Ningbo 315000, Zhejiang Province, P.R. China Y1 - 2016 PY - 2016 VL - 19 IS - 11 SP - 1245 EP - 1250 KW - Apoptosis KW - Caspase assay KW - Ginkgetin KW - JAK2/STAT3 KW - Renal cell carcinoma DO - 10.22038/ijbms.2016.7826 N2 - Objective(s): Renal cell carcinoma (RCC) is insensitive to conventional chemotherapy. Ginkgetin effectively treats several carcinoma cells. However, little is known about effects of Ginkgetin on RCC. In the present study, using 786-O cells, we evaluate whether Ginkgetin exerts anticancer effects against RCC. Materials and Methods: 786-O cells suspended in the medium containing Ginkgetin were cultured for 24 hr to 72 hr, and then MTT assay was used to study cytotoxic effect of Ginkgetin. Apoptosis in 786-O was measured by an FITC Annexin apoptosis detection kit. Protein expression was detected by Western blotting. 786-O cells with active Janus kinase 2 (JAK2)-Signal transducer and activator of transcription 3 (STAT3) were prepared by stimulant of interleukin-6 (IL-6), whereas 786-O cells with deactivated STAT3 were produced by small interfering RNA (siRNA) STAT3. Results: Ginkgetin suppressed the growth of 786-O in dose and time-dependent manners with IC50 values of 7.23 μM. Ginkgetin induced apoptosis of 786-O cells and increased the levels of caspase-8, caspase-9, and caspase-3. Additionally, Ginkgetin treated 786-O cells showed decreased levels of JAK2 and phosphorylated-STAT3 whether or not IL-6 was pretreated. Interestingly, pretreatment of siRNA STAT3 exerted inhibitory effects on the growth of 786-O cells, and the observation could be further reinforced after the Ginkgetin treatment. Conclusion: Our results indicate Ginkgetin possesses obvious inhibitory effects on the proliferation of 786-O, and this effect is probably due to its inhibition of JAK2/STAT3 pathway. Our findings imply Ginkgetin is a potential therapeutic medicine for RCC. UR - https://ijbms.mums.ac.ir/article_7826.html L1 - https://ijbms.mums.ac.ir/article_7826_b46f65feec946bdfcfd6e312d18ea1de.pdf ER -