TY - JOUR ID - 8090 TI - The effect of ghrelin on Kiss-1 and KissR gene transcription and insulin secretion in rat islets of Langerhans and CRI-D2 cell line JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Mahmoodzadeh Sagheb, Mandana AU - Azarpira, Negar AU - Mokhtary, Mokhtar AD - Department of Biology, Kazeroon Branch, Islamic Azad University, Kazeroon, Iran AD - Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Y1 - 2017 PY - 2017 VL - 20 IS - 1 SP - 36 EP - 40 KW - Diabetes Mellitus KW - ghrelin KW - Insulin KW - Islets of Langerhans KW - Kisspeptins DO - 10.22038/ijbms.2017.8090 N2 - Objective(s): Ghrelin is a peptide hormone that has been shown to have numerous central and peripheral effects. The central effects including GH secretion, food intake, and energy homeostasis are partly mediated by Kiss1- KissR signaling pathway. Ghrelin and its receptor are also expressed in the pancreatic islets. Ghrelin is one of the key metabolic factors controlling insulin secretion from the islets of Langerhans. We hypothesize that the inhibitory effect of ghrelin on KiSS-1 and KissR in the islet cells may be similar to the same inhibitory effect of ghrelin in the hypothalamus. Materials and Methods: To investigate the effect of ghrelin, we isolated the islets from adult male rats by collagenase and cultured CRI-D2 cell lines. Then, we incubated them with different concentrations of ghrelin for 24 hr. After RNA extraction and cDNA synthesis from both islets and CRI-D2 cells, the relative expression of KiSS-1 and KissR was evaluated by means of real-time PCR. Furthermore, we measured the amount of insulin secreted by the islets after incubation in different concentrations of ghrelin and glucose after 1 hr. Besides, we checked the viability of the cells after 24 hr cultivation.  Results: Ghrelin significantly decreased the KiSS-1 and KissR mRNA transcription in rat islets and CRI-D2 cells. Besides, Ghrelin suppressed insulin secretion from pancreatic beta cells and CRI-D2 cells. Conclusion: These findings indicate the possibility that KiSS-1 and KissR mRNA expression is mediator of ghrelin function in the islets of Langerhans. UR - https://ijbms.mums.ac.ir/article_8090.html L1 - https://ijbms.mums.ac.ir/article_8090_e92eeb53f73f9b55482cc4a9d8e8c8ef.pdf ER -