TY - JOUR ID - 8360 TI - Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Razeghian Jahromi, Iman AU - Mehrabani, Davood AU - Mohammadi, Ali AU - Ghahramani Seno, Mohammad Mahdi AU - Dianatpour, Mehdi AU - Zare, Shahrokh AU - Tamadon, Amin AD - Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, Iran AD - Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran AD - Division of Biotechnology, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran AD - Division of Biotechnology, Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Y1 - 2017 PY - 2017 VL - 20 IS - 3 SP - 301 EP - 307 KW - Bone marrow-mesenchymal- stem cells KW - Differentiation KW - Fetal brain extract KW - Neural cell KW - Vimentin DO - 10.22038/ijbms.2017.8360 N2 - Objective(s): Nowadays much effort is being invested in order to diagnose the mechanisms involved in neural differentiation. By clarifying this, making desired neural cells in vitro and applying them into diverse neurological disorders suffered from neural cell malfunctions could be a feasible choice. Thus, the present study assessed the capability of fetal brain extract (FBE) to induce rat bone marrow-derived mesenchymal stem cells (BM-MSCs) toward neural cells. Materials and Methods: For this purpose, BM-MSCs were collected from rats and cultured and their mesenchymal properties were confirmed. After exposure of the BM-MSCs to fetal brain extract, the cells were evaluated and harvested at days 3 and 7 after treatment. Results: The BM-MSCs that were exposed to FBE changed their appearance dramatically from spindle shape to cells with dendrite-like processes. Those neural like processes were absent in the control group. In addition, a neural specific marker, vimentin, was expressed significantly in the treatment group but not in the negative control group. Conclusion: This study presented the FBE as a natural neural differentiation agent, which probably has required factors for making neurons. In addition, vimentin overexpression was observed in the treated group which confirms neuron-like cell differentiation of BM-MSCs after induction. UR - https://ijbms.mums.ac.ir/article_8360.html L1 - https://ijbms.mums.ac.ir/article_8360_2d381972261cd956c8eb09467b65ef76.pdf ER -