TY - JOUR ID - 8585 TI - Effect of genistein on expression of pancreatic SIRT1, inflammatory cytokines and histological changes in ovariectomized diabetic rat JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Yousefi, Hadi AU - Alihemmati, Alireza AU - Karimi, Pouran AU - Alipour, Mohammad Reza AU - Habibi, Parisa AU - Ahmadiasl, Nasser AD - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AD - Department of Histology & Embryology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AD - Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Y1 - 2017 PY - 2017 VL - 20 IS - 4 SP - 423 EP - 429 KW - Diabetes KW - Genistein KW - Inflammatory cytokines-ovariectomy KW - SIRT1 DO - 10.22038/ijbms.2017.8585 N2 - Objective(s): Genistein is reported to have anti-diabetic and anti-inflammatory functions, in particular, direct effects on β-cell proliferation and insulin secretion. In this study, we investigated the anti-inflammatory effect of genistein on the pancreatic β-cells in ovariectomized diabetic rat. Materials and Methods:Forty female rats were divided into four groups: sham, bilateral ovariectomy (OVX), OVX.D (OVX+diabetes) and OVX.D.G (OVX.D+genistein). After bilateral ovariectomy, rats in the diabetic groups were fed high-fat diet (HFD), ad libitum for 4 weeks, and then a low dose of streptozotocin (STZ) (30 mg/kg) injected intraperitoneally. Genistein (1 mg/kg/day; SC) was administrated for 8 weeks. At the end of 8 weeks, pancreas tissue was removed and used for western blotting and Hematoxylin-Eosin staining. Results: Treatment with genistein declined inflammation and tissue injury, and this decline was correlated with the expression of SIRT1. OVX and OVX.D significantly increased Nf-кB and IL-1β expression and decreased SIRT1 levels compared to sham group (P<0.05). Significant reduction of Nf-кB and IL-1β, and increasing of SIRT1 were observed during genistein treatment (P<0.05). Conclusion: Estrogen deficiency alone or with HFD increased pancreatic inflammation. However, subcutaneous administration of gtenistein prevented from these inflammatory changes in the pancreas of a surgery animal model of ovariectomy with or without diabetes. Our results support the potential preventing effect of genistein from pancreatic injury. UR - https://ijbms.mums.ac.ir/article_8585.html L1 - https://ijbms.mums.ac.ir/article_8585_7f7493ee63a8324631016b4c678fa836.pdf ER -