TY - JOUR ID - 8834 TI - Establishment of human hair follicle mesenchymal stem cells with overexpressed human hepatocyte growth factor JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Zhou, Dan AU - Cheng, Hongjing AU - Liu, Jinyu AU - Zhang, Lei AD - Department of Pediatrics, The Second Hospital of Jilin University, Changchun 130041, China AD - Department of Gastroenterology, The First Hospital of Jilin University, Changchun 130041, China AD - The Ministry of Education Key Laboratory, The College of Basic Medical Sciences, of Jilin University, Changchun 130041, China Y1 - 2017 PY - 2017 VL - 20 IS - 6 SP - 662 EP - 675 KW - Hair follicle KW - HGF KW - Lentivirus KW - Liver diseases KW - Stem cells DO - 10.22038/ijbms.2017.8834 N2 - Objective(s): Chronic liver disease has become a major health problem that causes serious damage to human health. Since the existing treatment effect was not ideal, we need to seek new treatment methods. Materials and Methods: We utilized the gene recombination technology to obtain the human hair mesenchymal stem cells which overexpression of human hepatocyte growth factor (hHGF). Furthermore, we verified the property of transfected cells through detecting surface marker by flow cytometry. Results: We show here establishment of the hHGF-overexpressing lentivirus vector, and successfully transfection to human hair follicle mesenchymal stem cells. The verified experiments could demonstrate the human hair follicle mesenchymal stem cells which have been transfected still have the properties of stem cells. Conclusion: We successfully constructed human hair follicle mesenchymal stem cells which overexpression hHGF, and maintain the same properties compared with pro-transfected cells. UR - https://ijbms.mums.ac.ir/article_8834.html L1 - https://ijbms.mums.ac.ir/article_8834_8196218be98684140fa62d5500c2fc2d.pdf ER -