TY - JOUR ID - 9105 TI - Frequency of bap and cpaA virulence genes in drug resistant clinical isolates of Acinetobacter baumannii and their role in biofilm formation JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Fallah, Arezoo AU - Ahangarzadeh Rezaee, Mohammad AU - Hasani, Alka AU - Soroush Barhaghi, Mohammad Hossein AU - Samadi Kafil, Hossein AD - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AD - Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AD - Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AD - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Y1 - 2017 PY - 2017 VL - 20 IS - 8 SP - 849 EP - 855 KW - Acinetobacter baumannii KW - Antibiotic resistance KW - Biofilm KW - Bap KW - CpaA DO - 10.22038/ijbms.2017.9105 N2 - Objective(s): Acinetobacter baumannii has a high propensity to form biofilm and frequently causes medical device-related infections with multiple-drug-resistance in hospitals. The aim of this work is to study antimicrobial resistance and the role of bap and cpaA genes in biofilm formation by A. baumannii to understand how this pathogen persists in the hospital environment. Materials and Methods: Theantibiotic resistance profile and in vitro biofilm-forming ability of one hundred clinical isolates of A. baumannii was evaluated by disc diffusion and crystal-violet staining methods, respectively. Isolates were tested for the presence of bap and cpaA genes. Results: The isolates were highly resistant to cefepime, third-generation cephalosporins, ciprofloxacin, cotrimoxazole, aminoglycosides and carbapenems. Moreover, four isolates were resistant to colistin. Quantification of biofilm showed that 43% of the isolates were strong biofilm-producer. Furthermore, 32% of the isolates exhibited moderate biofilm-formation and showed initial binding activity. Frequency of bap and cpaA were determined 92% and 36%, respectively. Conclusion: There was strong association between the presence of bap gene and biofilm formation by A. baumannii isolates (P=0.003). In addition, multidrug resistant isolates produced stronger biofilm than other isolates (P=0.0001). These results indicate importance of biofilm in resistance of isolates and effect of presence of bap gene in biofilm formation by A. baumannii strains. UR - https://ijbms.mums.ac.ir/article_9105.html L1 - https://ijbms.mums.ac.ir/article_9105_3b2a950158283cc138a4f10d90a72758.pdf ER -