TY - JOUR ID - 9275 TI - Presenting a rapid method for detection of Bacillus cereus, Listeria monocytogenes and Campylobacter jejuni in food samples JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Razei, Ali AU - Sorouri, Rahim AU - Mousavi, Seyed Latif AU - Nazarian, Shahram AU - Amani, Jafar AU - Aghamollaei, Hosien AD - Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran AD - Department of Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran AD - Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran AD - Biology Research Centre, Faculty of Basic Science, Imam Hossein University, Tehran, Iran AD - Applied Microbiology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran Y1 - 2017 PY - 2017 VL - 20 IS - 9 SP - 1050 EP - 1055 KW - Bacillus cereus KW - Campylobacter jejuni KW - Hly KW - Listeria monocytogenes KW - Multiplex PCR KW - NHEB/NHEC DO - 10.22038/ijbms.2017.9275 N2 - Objective(s): Listeria monocytogens, Bacillus cereus and Campylobacter jejuni are three toxin producing bacteria over the world, especially in Iran, and it is essential to find a certain, rapid procedure to identify these microorganisms. In this research, these bacteria were simultaneously detected by multiplex PCR technique in foods. Materials and Methods: The primary approval of bacterial strains was performed by biochemical tests. PCR primers were designed based on the nucleotide sequences of the NHEB/NHEC gene of B. cereus, the hly gene of L. monocytogenes and the C gene of C. jejuni. The specificity of Multiplex PCR method was determined using seven food poisoning bacteria including Salmonella typhi, Shigella dysentery, Yersinia pestis, Staphylococcus aureus, Clostridium perfringens, Clostridium botulinum and Vibrio cholerae. To confirm the reaction, DNA extraction was performed from 30 food samples (milk), and gene amplification was performed by PCR. The length of amplified fragments was 300 bp, 210 bp and 160 bpfor NHEB/NHEC, hly and C genes, respectively. Results: The detection limits of the PCR method were 5, 4 and 3 pg for L. monocytogenes, B. cereus and C. jejuni, respectively. Specifisity test showed that this reaction is spesific to these 3 bacteria. Conclusion: In this study, we  introduced a new multiplex PCR method for simultsnus detection of L. monocytogens, B. cereus and C. jejuni. These results can be used  for detection of other toxin producing bacteria in food. UR - https://ijbms.mums.ac.ir/article_9275.html L1 - https://ijbms.mums.ac.ir/article_9275_c2643e4160267a68ff0687fcc3a3645f.pdf ER -